Team:Shanghai HS United/Improve
Type IIS standard assembly improvement
(1)GFP sequence remove Bsa I site
(2)Opertimize and measure the RBS sequence strength (Collaboration with Xiamen_City)
The results showed that BBa_B0034 RBS in type IIS assembly has low expression efficiency, and the RBS-A was the best.
(3)Blue-white sreening plasmid for Type IIS assembly
Cas12a detection methods improvement (compare to SHERLOCK and HOLMES method)
Compared to other CRISPR detection methods, our method has the merit of low-cost, convenience, sensitivity.
Reference
Li Y, Li S, Wang J, et al. CRISPR/Cas systems towards next-generation biosensing[J]. Trends in biotechnology, 2019.
Cas12 detection methods improvement (compare to other iGEM team)
In 2018, serveral iGEM teams use Cas12 or Cas13 in their project. Our project focuses on the ASFv detection.
