Team:ShanghaiTech China/Characterization
We have characterized the BBa_K2152004 from 2016 ShanghaitechChina_B which is a kill switch. (http://parts.igem.org/Part:BBa_K2152004) We utilized this part as a kill switch in our project.
We detected the OD600 of the part after the addition of IPTG in 6 hours and got a graph. Also, we detected the escaping efficiency of the part. (For more detail, visit https://2019.igem.org/Team:ShanghaiTech_China/Safety).
This graph shows the OD600 changes after addition of IPTG in six hours. Pink lines stand for experiment groups (with Gene E) and blue ones stand for control groups (without Gene E). Different numbers stand for different concentrations of IPTG: C0 - control group with no IPTG; C0.1 - control group with IPTG of 0.1mM; C0.5 - control group with IPTG of 0.5mM; C1 - control group with IPTG of 1.0mM; E0 - experiment group with no IPTG; E0.1 - experiment group with IPTG of 0.1mM; E0.5 - experiment group with IPTG of 0.5mM; E1 - experiment group with IPTG of 1.0mM.
IPTG(+) groups are plates with IPTG, IPTG(-) groups are plates without IPTG. In the picture above, we can clearly see the difference in the red circled 10-7 groups where IPTG(+) group only has 1 bacterial colony while IPTG(-) group has over 10 colonies.