Team:REC-CHENNAI/Characterization-Cytotoxic
CYTOTOXIC PROPERTY
When HeLa cells were treated with a lower concentration of the peptide (4-32 µM/ml) for 24h, Ltc-2a Wt showed ~20% cytoxicity, whereas Ltc-2a Mt showed ~10% cytotoxicity. There was a twofold decrease in the cytotoxicity of Ltc-2a Mt when compared to Ltc-2a Wt at lower concentrations of the peptide.
When HeLa cells were treated with a higher concentration of the peptide (64,120 µM/ml) for 24h, both Ltc-2a Wt and Ltc-2a Mt showed comparable levels of cytotoxicity of ~22%. This is attributed to the high concentration of the peptides.
When HeLa cells were treated with a lower concentration of the peptide (4-32 µM/ml) for 48h, Ltc-2a Wt showed ~65% cytoxicity, whereas Ltc-2a Mt showed ~30% cytotoxicity. There was a twofold decrease in the cytotoxicity of Ltc-2a Mt when compared to Ltc-2a Wt at lower concentrations of the peptide.
When HeLa cells were treated with higher concentration of the peptide (64,120 µM/ml) for 48h, Ltc-2a Wt showed ~95% Cytotoxicity, whereas Ltc-2a Mt showed ~70% Cytotoxicity. The mutant peptide showed only a slightly lower cytotoxic activity when compared to the native peptide owing to the higher concentration of the peptide added.
When C2C12 cells were treated with a lower concentration of the peptide (4-32 µM/ml) for 24h, both Ltc-2a Wt and Ltc-2a Mt showed ~24% cytotoxicity. Ltc-2a Wt and Ltc-2a Mt showed the same level of toxicity to C2C12 cells at lower concentrations of the peptide for 24h.
When C2C12 cells were treated with a higher concentration of the peptide (64,120 µM/ml) for 24h, Ltc-2a Wt showed ~75% cytotoxicity, whereas Ltc-2a Mt showed ~55% cytotoxicity. There was a slight reduction in cytotoxicity of Ltc-2a Mt when compared to Ltc-2a Wt at higher peptide concentrations in C2C12 cells.
When the C2C12 cells were treated with a lower concentration of the peptide (4-32 µM/ml) for 48h, both Ltc-2a Wt and Ltc-2a Mt showed ~65% cytotoxicity. Both Native and Ltc-2a Mt showed the same toxicity to C2C12 cells when treated at lower concentrations of the peptide for 48h.
When the C2C12 cells were treated with a higher concentration of the peptide (64,120 µM/ml) for 48h, both Ltc-2a Wt and Ltc-2a Mt showed ~95% cytotoxicity. Ltc-2a Wt and Ltc-2a Mt showed the same level of toxicity to C2C12 cells when treated at higher concentrations of the peptide for 48h.
