Team:RDFZ-China/Result

• Home
• Project
  Description Design Demonstrate Search Engine
• Model
• Human Practice
  Integrated Public Engagement & Education Future Plan
• Part
  Part collection Basic Parts
• Safety
• Laboratory
  Result Protocol Notebook
• Team
  Collaboration Members Attribution & Acknowledgement School
• Achievement

Result

For Characterization of qualitative data on part BBa_K2937000, where IPTG conc is variable, the response of fluorescent intensity of sfgfp:

For library construction, the team have successfully gathered parts from numerous previous projects, and the products are being tested by pcr:

As for cell free system protein expression, we can identify fluorescence by viewing green light after incubating in cell free system mix. SDS-PAGE is also conducted to identify GFP.

Fig.1

Fig 1 is cell free system expression SDS-PAGE demonstration. The sample amount in the gel was 2.5 uL. M is protein marker, the molecular weight unit is kD

Fig.2

In fig 2, the left one is the expression of the GFP sample, and the right one is the GFP positive control sample.