Result
For Characterization of qualitative data on part BBa_K2937000, where IPTG conc is variable, the response of fluorescent intensity of sfgfp:
![](https://static.igem.org/mediawiki/2019/0/05/T--RDFZ-CHina--result1.png)
![](https://static.igem.org/mediawiki/2019/0/0a/T--RDFZ-CHina--result2.png)
For library construction, the team have successfully gathered parts from numerous previous projects, and the products are being tested by pcr:
![](https://static.igem.org/mediawiki/2019/0/06/T--RDFZ-CHina--result3.png)
As for cell free system protein expression, we can identify fluorescence by viewing green light after incubating in cell free system mix. SDS-PAGE is also conducted to identify GFP.
![](https://static.igem.org/mediawiki/2019/1/19/T--RDFZ-CHina--result4.png)
Fig.1
Fig 1 is cell free system expression SDS-PAGE demonstration. The sample amount in the gel was 2.5 uL. M is protein marker, the molecular weight unit is kD
![](https://static.igem.org/mediawiki/2019/2/29/T--RDFZ-CHina--result5.png)
Fig.2
In fig 2, the left one is the expression of the GFP sample, and the right one is the GFP positive control sample.
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