Team:RDFZ-China/Achievement

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• Project
  Description Design Demonstrate Search Engine
• Model
• Human Practice
  Integrated Public Engagement & Education Future Plan
• Part
  Part collection Basic Parts
• Safety
• Laboratory
  Result Protocol Notebook
• Team
  Collaboration Members Attribution & Acknowledgement School
• Achievement

We are qualified for iGEM Bronze medal for:

Having registered for iGEM, having a great 2019 iGEM season, and being registered for the Giant Jamboree;

Having completed the all the deliverables: wiki, poster, presentation, judging form and attributions;

Having submitted our project’s inspiration and description;

Having the part BBa_180000 characterized;

We are qualified for iGEM Silver medal for:

Achieving all the criteria which are required for Bronze medal;

Validating the part(s) BBa_297000, BBa_K297001, BBa_K297002, BBa_K297003, BBa_K297004;

Collaborating with BNDS-China, UCAS, and BHSF_ND teams;

And having a fantastic human practice program;

We are qualified for iGEM Gold medal for:

Achieving all the criteria which are required for silver and bronze medal;

Improving parts BBa_K2656009 and BBa_K18000, to part BBa_K2937000 successfully;

Having an amazing integrated HP program, and the program has educational value;

Having mathematical modeling of our results in the experiment we’ve done;

Demonstrating the project will work by characterizing promoter region of the submitted composite part successfully and having quantitative data on gene expression of gfp in place of fragrance generates protein coding sequence, in terms of fluorescent intensity, which validates that the system is feasible;

Characterizations on new parts are changed in competence cell for enzyme expression to construct the series of Ehrlich pathway;

The use of SDS-PAGE for the identification of involved proteins;

Conduction of cell free system of protein expression for expression validation;

We also wanted to apply for two special prizes: integrated human practice and education and public engagement.

We are striving to make a good and integrated human program. Since we are constructing a library of fragrance parts from previous iGEM teams, we are required to search for a lot of resources. While searching on iGEM website for fragrance-generating parts, we underwent an exhausting and time consuming process due to the scattered information in excessive project webpages where detailed descriptions were usually devoid. After interviewing some researchers and professors in our laboratory, we consolidated the fact that the problem of loosely distributed information existed throughout most scientific researching process. Therefore, we decided to construct a library of fragrance-generating genes to solve the problem. To confirm the effectiveness of our library, we invited several scientists and iGEM participants and get a fair number of affirmative as well as constructive feedbacks. Based on these feedback, we simplified our page and enlarged our coveraging area for some specific typings and thus made our library better.

We are also trying our best to make good use of our human practice program to cash educational values and good engagement in the public society. As traditional demonstrations of bioengineering processes are always considered "expertise", the concept of cloning is poorly perceived by public. Therefore, we identified an approach that can simplify the demonstration in a straightforward way. Standing as an educational tool, cell free system is capable of revealing results of experiments without intricate operations. Within several self-explanatory steps, by which coding sequence on the plasmid can be expressed, the concept of cloning could be delivered clearly. Smell of odorants particles is an obvious sensation which offers a more figurative explanation. To further disseminate laboratory operations, we compiled an experiment instruction booklet as a training material for students and participants in iGEM high school committee.