number
name>
type
description
source
pan promoter
procaryotic promoter
Pan is a promotor from Bacillus subtilis that behaves as a strong promoter when used in Cupriavidus metallidurans. This sequence also contains a ribosome binding site.
[1]
pbrR upstream region
DNA
~300nt downstream region of pbrR gene of Cupriavidus metalidurans, delimited by BglII and NdeI sites, for further insertion in pCM184 plasmid [2] in perspective of homologeous recombinaison.
[3]
pbrR downstream region
DNA
~300nt downstream region of pbrR gene of Cupriavidus metalidurans, delimited by ApaI and HpaI sites, for further insertion in pCM184 plasmid [2] in perspective of homologeous recombinaison.
[3]
References
[1] - Ribeiro-dos-Santos, G., Biondo, R., Quadros, O. de F., Vicente, E. J., & Schenberg, A. C. G. (2010). A metal-repressed promoter from gram-positive Bacillus subtilis is highly active and metal-induced in gram-negative Cupriavidus metallidurans. Biotechnology and Bioengineering, 107(3), 469–477. https://doi.org/10.1002/bit.22820
[2] - Broad-host-range cre-lox system for antibiotic marker recycling in gram-negative bacteria. Marx CJ, Lidstrom ME. Biotechniques. 2002 Nov;33(5):1062-7. 10.2144/02335rr01 PubMed 12449384
[3] - https://www.ncbi.nlm.nih.gov/nuccore/NC_007973 on 04/09/2019
[4] - http://parts.igem.org/Part:BBa_K1122673 on 04/09/2019