Team:Nanjing High School/Parts

The characterization protein and early diagnosis of cervical cancer

Here we demonstrate specific protein expression variations related to NFX1 over-expression, a gene found to be closely associated with cervical cancer.

Parts

PARTS 
Parts Overview
Our project is a public welfare project about blue light monitoring. The core of the project is an engineered Escherichia coli with light-catch system (the expression of optically controlled inhibitory protein, thus regulating the probability of Cas1-Cas2 capturing DNA fragments). The time of exposure to light of Escherichia coli is determined by PCR. In the follow-up promotion, we will make such microorganisms into an ornament to wear on people. After a certain period of time, the samples are returned to the lab, where the length of the Escherichia coli growth is read to infer how long the blue light was exposed.
So, our parts have two systems, one is blue light sensor (including gene YF1, FixJ and promoter Fixk2), another is Catch system (including gene Cas1, Cas2 and Tfus Cas3).

Basic Parts

Composite Parts

 

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