Favorate Parts
CHoRE (BBa_K3064000)
It is a binding site of CHREBP. Carbohydrate response element binding protein (ChREBP) can bind with CHoRE and regulate the expression of key genes involved in various pathways including glycolysis, gluconeogenesis and lipogenesis.
Figure 1: ChREBP bind with ChoRE
9XGlucose Sensing Promoter(BBa_K3064026)
In order to improve this part, this year we have made a series of modification based on the Minimum TATA-box promoter designed by Daniel Tang of Stanford BIOE44 - S11.(BBa_M50098). Due to the low efficiency of TATA box promoter, we shorten the sequence into only minp. In addition, we also added glucose-sensing fragment to enhance the part’s initiation strength, as well as glucose-sensing function. With GFP, the part’s function can be better detected.
Figure 2. The structure diagram of the 9xGSP-GFP part.
Parts List