Monthly progress & Milestones
2018 November
● Summary for 2018 NUDT_CHINA in iGEM.
2018 December
● 2019 NUDT_CHINA team set-up.
2019 January
● Brainstorm for 2019 team project. Anchor T2D as the field we want to anchor our project.
● Background information gathering.
● Team member split into groups: Wet Lab, Human Practice, Wiki design, Model.
2019 February
● Nurse interview and visit in geracomium to gather realistic T2D information and publicity of good lifestyles for the elders to avoid or retard T2D complication.
● Determine 2019 project --- continue with PR Predator in 2018, but focus on the degradation of glucagon receptor in hepatocytes.
● Look up for authority articles.
● Experimental skills and safety training for team members in Wet Lab.
2019 March
● Crucial parts construction.
2019 April
● First milestone in project --- designed gene circuit works well in degrade GCGR in HepG2 cell line.
● Diabetes Knowledge Popularization Activity.
● Public questionnaire about diabetes.
2019 May
● Expert interview on our subject. Question raised: What if the CMV promoter keeps working, would this circuit result in hypoglycemia in human body?
● Internet articles research.
● Improvement of project: involvement of glucose sensing promoter.
2019 June
● Experiment protocol gathering and design.
● Glucose sensing promoter related circuit construction.
● Model preparation.
2019 July
● Glucose sensing promoter functional test in HepG2.
2019 August
● 2019 iGEM Chongqing Meet-up.
●Second milestone: Result for glucose sensing promoter functional test.
●Determination: Mouse primary hepatocytes as platform for continuous experiments of designed GCGR degradation circuit. Adenovirus as circuit transport tool.
●Research, discussion and review for safety concerns on adenovirus and mouse primary hepatocytes.
2019 September
● Collaboration with CSU_CHINA about current gene delivery methods and new ways to improve delivery efficiency as well as decrease cell toxicity.
●Third milestone: Result for glucose sensing enabled GCGR degradation circuit in HepG2.
2019 October
● Fourth milestone: Result for mouse primary hepatocytes.
●Fifth milestone: Model for evaluate the effectiveness of initial design and indicate the potentials of circuit in long-term in vivo uses.
●Preparation for team wiki, poster, presentations and the Giant Jamboree!
