NAU-iGEM

Part Collection

Overview

This year, NAU-CHINA build 18 parts, including 14 basic parts, 4 composite parts. We build 9 anti-Plasmodium peptides, and optimized these codon, so that make it more suitable to express in Serratia marcescens. If you want to see more details, you can find the usage and characterization in the part registry page. You can also visit the basic part, composite part, part collection and improved part page for more information.

Basic parts

This year, NAU-CHINA build 14 basic parts, including 9 single anti-Plasmodium peptide, HasA hemophore, TEV cleavage site, TEV protease, the fusion anti-plasmodium protein coding sequence and A/U-rich 5’-UTR. You can click on the part number for more detail in the following table.

Part Number	Name	Type
BBa_K3139000	Anti-plasmodium peptide MeuTXKβ1	Coding
BBa_K3139001	A/U-rich 5’-UTR	DNA
BBa_K3139002	Anti-plasmodium peptide drosomycin	Coding
BBa_K3139003	Anti-plasmodium peptide Gomesin	Coding
BBa_K3139004	Anti-plasmodium peptide Shiva-3	Coding
BBa_K3139005	Anti-plasmodium peptide MP2	Coding
BBa_K3139006	Anti-plasmodium peptide AdDLP	Coding
BBa_K3139007	Anti-plasmodium peptide Vida3	Coding
BBa_K3139008	Anti-plasmodium peptide SM1	Coding
BBa_K3139009	Anti-plasmodium peptide Thiostrepton	Coding
BBa_K3139010	HasA hemophore coding sequence	Coding
BBa_K3139011	TEV protease recognition and cleavage site	Coding
BBa_K3139012	TEVpS	Coding
BBa_K3139013	Anti-plasmodium fusion protein	Coding

Composite Parts

We combined basic parts, promoters, terminators and RBS, which are presented in the following table. You can click on the part number to see the details.

Part Number	Name	Type
BBa_K3139014	J23100-fusion protein-B0015-J23106-TEVp-TEVp site-HasA-B0010	Composite
BBa_K3139015	J23100-fusion protein-B0015-J23110-TEVp-TEVp site-HasA-B0010	Composite
BBa_K3139016	J23100-fusion protein-B0015-J23111-TEVp-TEVp site-HasA-B0010	Composite
BBa_K3139017	RBS measurement device J23100 K3139001 B0032 RFP	Measurement

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Improved part

This year, NAU-CHINA improved 1 measurement part by using mutation of the 5’-UTR (Untranslated Region), selecting the most effective one to be inserted between the promoter and RBS sequence, which increased the florescent intensity to approximately 2-fold.

Part Number	Name	Type
BBa_K3139017	RBS measurement device J23100 K3139001 B0032 RFP	Measurement
BBa_K528004	RBS measurement device J23100 B0032 RFP	Measurement

Part collection

This year, NAU-CHINA designed mainly 3 parts as a collection. By using different combinations of promoters with both TEV protease (BBa_K3139012) and Anti-plasmodium fusion protein (BBa_K3139013), we explored the best combination of their expression strength, which led the least waste of TEV protease and the least pre-fusion protein with incomplete cleavage.