Team:NAU-CHINA/Notebook

NAU-iGEM

Notebook


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Notebook

2019 January

Set up NAU-CHINA 2019!


2019 February - March

Completed periodical literature retrieval and brainstormed for project design.
Consulted Professor Ai Xia about our project design.
Determined the final topic--malaria averting.


2019 April

Retrieved and read the papers of anti-Plasmodium peptides and HasA secretion system.
Visited Yunnan Institute of Parasitic Diseases to consult the experts with some details of our project.


2019 May

Conducted new team’s experimental skills training.
Established the preliminary digital model.
Consulted Professor Yiping Wang to find and access an efficient protease.


2019 June

Constructed the plasmid and conducted experimental training.
Promoted NAU-iGEM Campus Competition.
Held Agar Art Campus Competition.


2019 July

Held Nanjing Science and Technology Museum Promotion.
Constructed standard part plasmid.
Constructed the H1yA-J04450 plasmid.


2019 August

Constructed standard part plasmid.
Conducted homologous recombination.
Sought Serratia marcescens’ best electro transformation condition.
Preliminarily verified TEVp’s cutting effect.
Tested DH5a’s secretory system.
Attended the CCIC conference.
Conducted model improvement.


2019 September

Held the YuLan-Road Promotion.
Constructed a replacement promoter plasmid and secretion of HasA plasmid.
Verified the secretory effect of electro transformed Serratia marcescens.
Constructed newly adjust plasmid for DH5a and Serratia marcescens.
Perfected the secretory system of HlyA.
Improved cellular automaton model.
Edited Wiki.


2019 October

Analyzed data.
Used Western Blot to test the secretion effect.
Tested the J04450 standard part.
The closing ceremony.
Completed model improvement.
Edited Wiki.


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