Team:Linkoping Sweden/Primers
Primers
Primers used by iGEM19 Linköping
Table 1. Primers used for all experiments conducted by iGEM19 Linköping.
| Name | Sequence (5'-3') | Direction | Use |
|---|---|---|---|
| Insert Forward | atggtacgaattcgcggccgcttctagag | Forward | Amplification of inserts (Biobrick RFC10) |
| Insert Reverse | gaagcatctgcagcggccgctactagta | Reverse | Amplification of inserts (Biobrick RFC10) |
| BamHI-F | tcgactccagcatccggatcc | Forward | Amplification of fusion protein inserts |
| Vector Forward | cggcatctctagaagcggccgcgaattc | Forward | Amplification of pSB1C3 (Biobrick RFC10) |
| Vector Reverse | agtgcatactagtagcggccgctgcag | Reverse | Amplification of pSB1C3 (Biobrick RFC10) |
| CBD-F | caaggacgttttgccaagaac | Forward | Colony screening / Sequencing |
| VF2 | tgccacctgacgtctaagaa | Forward | Colony screening / Sequencing |
| VR | attaccgcctttgagtgagc | Reverse | Colony screening / Sequencing |
