Basic partsDown below is our basic part collection. All basic parts were designed to be able to assemble with BBa_K3182001 and our expression system, BBa_K3182200. To be able to assemble our basic parts with our cellulose binding domain (CBDcipA) we added a BamHI site inside the linker region of our construct, see Figure 1. The BamHI site was chosen because of it being a part of the RFC21, and coding for one glycine and one serine which is the end sequence of the thrombin cleavage site. The thrombin cleavage site was added into the linker region to be able to separate our fusion proteins. We had no trouble assembling our fusion proteins using the pink-white screening method (BBa_K3182100).
Table 2. Basic parts used in the wet lab part of our project. All basic parts used was designed to fit for assembly with BBa_K3182001.
|Biobrick ID||Short name||Function||Size||Designed by|
|BBa_K3182001||CBDcipA||Carbohydrate binding domain with a C-terminal linker||516 bp||Oliver Hild Walett, Johan Larsson|
|BBa_K3182003||PlyF307-SQ8C||Acinetobacter genus lysin||444 bp||Oliver Hild Walett|
|BBa_K3182004||CHAP(K)||Staphylococcus genus lysin||489 bp||Leo Juhlin|
|BBa_K3182005||LL-37||Antibacterial peptide||117 bp||Oliver Hild Walett|
|BBa_K3182006||Magainin 2||Antimicrobial peptide||75 bp||Oliver Hild Walett|
|BBa_K3182007||Pln1||Antibacterial peptide||117 bp||Oliver Hild Walett|
|BBa_K3182009||Parseqα||Synthetic antimicrobial peptide||60 bp||Johan Larsson, Jonatan Baggman|
|BBa_K3182010||Parseqβ||Synthetic antimicrobial peptide||60 bp||Johan Larsson, Jonatan Baggman|
|BBa_K3182013||mNeonGreen||Fluorescent protein||771 bp||Oliver Hild Walett, iGEM Linköping 2018|