Team:Linkoping Sweden/Composite Part
Composite parts
Composite parts
Our composite parts all have the same elements. We utilized the strongest promotor for Escherichia coli (E. coli), the phage derived T7 promotor. And by also adding other elements, which are untranslated, an even higher expression of our composite parts could be achieved (BBa_K1758100). The effects of this expression system over a native-driven one can be seen in our improved part section. The expression of our antimicrobial agents was successful and the fusion proteins could be found overexpressed on SDS-PAGE gels.
Table 1. All the composite parts was used in the wet lab part of our project. All team members worked with our composite parts.
| Biobrick ID | Short name | Function | Size | Designed by |
|---|---|---|---|---|
| BBa_K3182000 | pT7-CBDcipA-AsPink | Characterization of the carbohydrate binding domain | 1287 bp | Oliver Hild Walett |
| BBa_K3182100 | pT7-CBDcipA-pCons-AsPink | Carbohydrate binding domain assembly vector | 1334 bp | Oliver Hild Walett |
| BBa_K3182200 | pT7-CBDcipA | Base fusion vector for peptides and enzymes | 585 bp | Oliver Hild Walett |
| BBa_K3182103 | pT7-CBDcipA-PlyF307-SQ8C | CBD fusion - Acinetobacter genus lysin | 1029 bp | Oliver Hild Walett |
| BBa_K3182104 | pT7-CBDcipA-CHAP(K) | CBD fusion - Staphylococcus genus lysin | 1074 bp | Oliver Hild Walett, Leo Juhlin |
| BBa_K3182105 | pT7-CBDcipA-LL-37 | CBD fusion - Antibacterial peptide LL-37 | 702 bp | Oliver Hild Walett |
| BBa_K3182106 | pT7-CBDcipA-Magainin 2 | CBD fusion - Antimicrobial peptide MG2 | 660 bp | Oliver Hild Walett |
| BBa_K3182107 | pT7-CBDcipA-Pln1 | CBD fusion - Antibacterial peptide Pln1 | 702 bp | Oliver Hild Walett |
| BBa_K3182108 | pT7-CBDcipA-sfGFP | Characterization of the carbohydrate binding domain | 1305 bp | Oliver Hild Walett |
