Team:Lethbridge/Experiments


Experiments


Experimentation was an integral part of developing Algulin. We tested protein expression in E. coli and in microalgae. This was done to have a comparison for protein production between the organisms using the same constructs in both. To make an adequate product our team needed to select a viable chassis. In the selection process for an adequate chassis, we tested C. merolae, C. reinhardtii, and A. platensis in a variety of experiments to test cell integrity. For an overall description of our experiments, visit our Experimental Design page. For more information on our experiments please see our protocols below.



Protocols

Algae degradation protocol

Algae liquid media protocol

Bacterial transformation protocol

Chlamydomonas transfomation

Colony PCR

DNA purification

Gblock PCR

LB media

Ligation

PCR product purification

Phusion PCR

Plasmid miniprep

Protein overexpression

Purification of His-tagged E. coli

Restriction enzyme digest

Touch Down PCR

Affinity purification

Lyophilization

Plate reader protocol