Experiments
Experimentation was an integral part of developing Algulin. We tested protein expression in E. coli and in microalgae. This was done to have a comparison for protein production between the organisms using the same constructs in both. To make an adequate product our team needed to select a viable chassis. In the selection process for an adequate chassis, we tested C. merolae, C. reinhardtii, and A. platensis in a variety of experiments to test cell integrity. For an overall description of our experiments, visit our Experimental Design page. For more information on our experiments please see our protocols below.
Protocols
Bacterial transformation protocol