Team:Lethbridge/Composite Part


Composite Parts



Each of the following parts was submitted to the iGEM Registry. This year, we have designed a variety of different constructs for validating two insulin varieties: proinsulin peptides and the ultrastable single-chain insulin, SCI57, in E.coli and C. reinhardtii.


All of E.coli parts were designed with the same constitutive promoter, (BBa_J23119), RBS (BBa_J61100) and terminator (BBa_B0014) from the iGEM registry.


The algae constructs mostly have the constitutive promoter psaA and 5’UTR (BBa_K2148000) and the rbcL 3’UTR that can also be found in the Registry. The kill switch we have designed deviates from this main design in that on of the genes is under a inducable promoter called PcopM72 which we have entered into the registry and a already submitted double terminator BBa_B0015.


For an in depth explanation of our construct designs and overall system, please see our design page. For more construct details please check out our Parts Overview page or click on one of the parts below to be taken to our registry.



Part Number
Part Name
BBa_K3237013 Insulin protein receptor Pcnst.-RBS- 6XHis- Insulin Protein Receptor- dT
BBa_K3237014 His-mRFP1 codon optimized for E.coli Pcnst.-RBS-6XHis-RFP-dT
BBa_K3237015 His-mRFP1 codon optimized for C. reinhardtii psaA +5’UTR-6XHis-RFP-rbcL3’UTR
BBa_K3237016 His-mRFp1-Proinsulin with furin cut sites codon optimized for E.coli Pcnst.-RBS- 6XHis-mRFP1-proinsulin-dT
BBa_K3237017 His-mRFP1-Proinsulin with furin cut sites codon optimized for C. reinhardtii psaA + 5’UTR-6XHis-mRFP1-proinsulin-rbcL3’UTR
BBa_K3237018 His-mRFP1-SCI57 with furin cut sites codon optimized for E.coli Pcnst.-RBS- 6XHis-mRFP1-SC157-dT
BBa_K3237019 His-mRFP1-SCI57 with furin cut sites codon optimized for C. reinhardtii psaA + 5’UTR-6XHis-mRFP1-SC157-rbcL3’UTR
BBa_K3237020 His-mRFP1-Proinsulin-CPP with furin cut sites codon optimized for E.coli Pcnst.-RBS-6XHis-mRFP1-proinsulin-CPP-dT
BBa_K3237021 His-mRFP1-Proinsulin-CPP with furin cut sites codon optimized for C. reinhardtii psaA+5’UTR-6XHis-mRFP1-proinsulin-CPP-rbcL3’UTR
BBa_K3237022 His-mRFP1-SCI57-CPP with furin cut sites codon optimized for E.coli Pcnst.-RBS-6XHis-mRFP1-SC157-CPP-dT
BBa_K3237023 His-mRFP1-SCI57-CPP with furin cut sites codon optimized for C. reinhardtii psaA +5’UTR-6XHis-mRFP1-SC157-CPP-rbcL3’UTR
BBa_K3237024 NAB1 psaA+ 5’UTR-6XHis-NAB1-rbcL3’UTR
BBa_K3237028 nuiA PcopM72-nuiA-dT
BBa_K3237029 nucA psaA+ 5'UTR-nucA-dT