Team:Lethbridge/Basic Part
Basic Parts
Each of the following parts was submitted to the iGEM registry. This year, we have designed a variety of different constructs for validating two insulin varieties: proinsulin peptides and an ultrastable single chain insulin, SCI57, in E.coli and C. reinhardtii. We have also designed NAB1 constructs to manipulate light amounts needed for algae growth. Finally, we have also designed a kill switch to be implemented in case of environmental release.
For an in depth explanation of our construct designs and overall system, please see our Design page.
| BBa_K3237000 | Insulin protein receptor |
| BBa_K3237001 | His-mRFP1 codon optimized for E.coli |
| BBa_K3237002 | His-mRFP1 codon optimized for C. reinhardtii |
| BBa_K3237003 | His-mRFP1-Proinsulin with furin cut sites codon optimized for E.coli |
| BBa_K3237004 | His-mRFP1-Proinsulin with furin cut sites codon optimized for C. reinhardtii |
| BBa_K3237005 | His-mRFP1-SCI57 with furin cut sites codon optimized for E.coli |
| BBa_K3237006 | His-mRFP1-SCI57 with furin cut sites codon optimized for C. reinhardtii |
| BBa_K3237007 | His-mRFP1-Proinsulin-CPP with furin cut sites codon optimized for E.coli |
| BBa_K3237008 | His-mRFP1-Proinsulin-CPP with furin cut sites codon optimized for C. reinhardtii |
| BBa_K3237009 | His-mRFP1-SCI57-CPP with furin cut sites codon optimized for E.coli |
| BBa_K3237010 | His-mRFP1-SCI57-CPP with furin cut sites codon optimized for C. reinhardtii |
| BBa_K3237011 | NAB1 |
| BBa_K3237012 | NAB1 antisense |
| BBa_K3237025 | nuiA |
| BBa_K3237026 | nucA |
| BBa_K3237027 | PcopM72 promoter |