Team:Kyoto/Part Collection

Part Collection

This is a plastic-binding protein coding parts collection. This collection includes proteins that bind to polyethylene terephthalate (PET), polypropylene (PP), polyurethane (PU). Also, proteins can bind to various kinds of plastic materials such as fiber, cloth, and film. These plastic binding proteins are fused with SpyCatcher which can form isopeptide bond between SpyTag when they are mixed. Therefore, these proteins can be easily conjugated with proteins that have SpyTag. Through this SpyCatcher/SpyTag system, these proteins give plastic-binding ability to arbitrary type of protein. Furthermore, each protein has a character for binding targets. Some bind strongly to fibers and some to clothes. When giving a plastic-binding ability to other proteins, proteins can be chosen depending on the binding target.

Name	Type	Description	Function	Designer	Purification
BBa_K3185004	Protein coding	SpyCatcher -> BaCBM2	Plastic-binding protein	Masahiro Sakono	OK
BBa_K3185005	Protein coding	SpyCatcher -> CenA W68Y	Plastic-binding protein	Masahiro Sakono	OK
BBa_K3185006	Protein coding	SpyCatcher -> sfGFP ->LCI KR-2	Plastic-binding protein	Masaaki Shimazoe	OK
BBa_K3185007	Protein coding	SpyCatcher -> sfGFP -> TA2	Plastic-binding protein	Masaaki Shimazoe	OK
BBa_K3185008	Protein coding	GST -> SpyCatcher -> Hydrophobin	Plastic-binding protein	Masaaki Shimazoe	Failed
BBa_K3185009	Protein coding	SpyCatcher -> PETase	Plastic-binding protein	Masaaki Shimazoe	Failed
BBa_K3185010	Protein coding	SpyCatcher -> engineered PETase	Plastic-binding protein	Masaaki Shimazoe	Failed