Our best basic part is SpyTag inserted Tm Encapsulin.
(BBa_K3185000)
This part is a coding protein that can form a protein capsule as 60mer. Also, SpyTag is exposed on the surface of the capsule. This SpyTag enables to display SpyCatcher fused proteins on the protein capsule. SpyCatcher and SpyTag form isopeptide bond when they are mixed, so the protein displaying can be designed in vitro. For example, they can display several kinds of protein at the same time.
With SpyCatcher, this part will explore protein capsule-based synthetic biology!
Team:Kyoto/Basic Part
Basic Parts
Best Basic Part
Basic Part list
| Name | Type | Description | Function | Designer | Purification |
|---|---|---|---|---|---|
| BBa_K3185000 | Protein coding | SpyTag inserted TmEncapsulin | Biological polymer | Masaaki Shimazoe | OK |
| BBa_K3185001 | Protein coding | TmEncapsulin | Biological polymer | Masaaki Shimazoe | OK |
| BBa_K3185002 | Protein coding | sfGFP / TmEncapsulin | Biological polymer and loading protein | Masaaki Shimazoe | OK |
| BBa_K3185003 | Protein coding | SpyCatcher | Control | Masaaki Shimazoe | OK |
| BBa_K3185004 | Protein coding | SpyCatcher -> BaCBM2 | Plastic-binding protein | Masahiro Sakono | OK |
| BBa_K3185005 | Protein coding | SpyCatcher -> CenA W68Y | Plastic-binding protein | Masahiro Sakono | OK |
| BBa_K3185006 | Protein coding | SpyCatcher -> sfGFP ->LCI KR-2 | Plastic-binding protein | Masaaki Shimazoe | OK |
| BBa_K3185007 | Protein coding | SpyCatcher -> sfGFP -> TA2 | Plastic-binding protein | Masaaki Shimazoe | OK |
| BBa_K3185008 | Protein coding | GST -> SpyCatcher -> Hydrophobin | Plastic-binding protein | Masaaki Shimazoe | Failed |
| BBa_K3185009 | Protein coding | SpyCatcher -> PETase | Plastic-binding protein | Masaaki Shimazoe | Failed |
| BBa_K3185010 | Protein coding | SpyCatcher -> engineered PETase | Plastic-binding protein | Masaaki Shimazoe | Failed |
