Team:ITB Indonesia/Model
Enzyme model is created based on signalling phenomenon sequences of genetically engineered E.coli emits florescence in response to AHL stimulus.
In this system, AHL, describe as AHLe, produced by Vibrio parahaemolyticus and diffuses through the the bacteria membrane, becoming internal AHLi at rate kc. kc described as mass transfer constant through the membrane. The reaction forming dimer complex (C1) which will be reacting with a constitutive promoter, Pon, that drives the expression of the luxN gene that codes for the AHL receptor protein, at rate kLc. The dimer complex (C1) will reversibly binds to the promoter sequence, PLux, forming complex C2, which induces transcription and translation GFP. GFP then emits fluorescents. GFP can degrade at rates kGd, but this value the value can be ignored assuming there is no fluorescence degradation
k1 governs the association and dissociation of C1, k2 governs the association and dissociation of C2; kLc represents the constitutive rate of production of LuxN, kGd governs the rate of GFP degradation. This equation can be solved by using numerical method.
