Team:Bielefeld-CeBiTec/Oligos
Oligos
| ID | Name | Length | Tm °C | Sequence 5'-> 3' |
|---|---|---|---|---|
| 19aa | pSB_T7_r | 31 | 57.0 | CTAGTATTTCTCCTCTTTCTCTAGTATCTCC |
| 19ab | pSB_T7_H_f | 41 | 58.0 | CATCATCACCATCACCACTGATGATACTAGAGCCAGGCATC |
| 19ac | mC_pSB_f | 48 | 57.0 | GGGAGATACTAGAGAAAGAGGAGAAATACTAGATGGTGAGCAAGGGCG |
| 19ad | mC_pSB_r | 61 | 58.0 | GATGCCTGGCTCTAGTATCATCAGTGGTGATGGTGATGATGCTTGTACAGCTCGTCCATGC |
| 19ae | pTXB1_f | 17 | 57.0 | TGCATCACGGGAGATGC |
| 19af | pTXB1_r | 44 | 57.0 | CATATGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAG |
| 19ag | mC_pTXB1_f | 46 | 57.0 | GTTTAACTTTAAGAAGGAGATATACATATGATGGTGAGCAAGGGCG |
| 19ah | mC_pTXB1_r | 50 | 59.0 | TAGGGCAACTAGTGCATCTCCCGTGATGCACTTGTACAGCTCGTCCATGC |
| 19ai | pSB_H_f | 23 | 58.0 | CATCATCACCATCACCACTGATG |
| 19aj | mC_r | 20 | 58.0 | CTTGTACAGCTCGTCCATGC |
| 19ak | p8_mC_f | 57 | 58.0 | TCCACCGGCGGCATGGACGAGCTGTACAAGATGAAAAAGTCTTTAGTCCTCAAAGCC |
| 19al | p8_pSB_r | 52 | 58.0 | CTAGTATCATCAGTGGTGATGGTGATGATGGCTTGCTTTCGAGGTGAATTTC |
| 19am | p8_pTXB1_r | 52 | 59.0 | TAGGGCAACTAGTGCATCTCCCGTGATGCAGCTTGCTTTCGAGGTGAATTTC |
| 19an | sfGFP_f | 19 | 58.0 | ATGCGTAAAGGCGAAGAGC |
| 19ao | sfGFP_r | 26 | 59.0 | TTTGTACAGTTCATCCATACCATGCG |
| 19ap | t7prom_pSB_f | 66 | 60.0 | AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTAATACGACTCACTATAGGCGCGTTTATTCC |
| 19aq | crRNA_pSB_r | 62 | 61.0 | TTTGCCGGACTGCAGCGGCCGCTACTAGTATTTAAGGATGACGGCACGTATTTGATTTTCTG |
| 19ar | pSB1C3_f | 19 | 60.0 | TACTAGTAGCGGCCGCTGC |
| 19as | pSB1C3_r | 17 | 59.0 | CTCTAGAAGCGGCCGCG |
| 19at | pSB1C3_basicI_f | 25 | 58.0 | TACTAGTGAACCCGATGCAAATGAG |
| 19au | pSB1C3_basicI_r | 40 | 58.0 | CTAGTATATAGTTTTTTCTCCTTGACGTTAAAGTATAGAG |
| 19av | sfGFP_pGALL_f | 49 | 59.0 | TAACGTCAAGGAGAAAAAACTATATACTAGATGCGTAAAGGCGAAGAGC |
| 19aw | sfGFP_tTPS1_r | 59 | 59.0 | ATCGTCTCATTTGCATCGGGTTCACTAGTATCATTTGTACAGTTCATCCATACCATGCG |
| 19ax | mCherry_SUP4_r | 49 | 59.0 | AGACATAAAAAACAAAAAAACTAGTATTACTTGTACAGCTCGTCCATGC |
| 19ay | mCherry_SNR52_f | 46 | 59.0 | CTCCGCAGTGAAAGATAAATGATCTACTAGATGGTGAGCAAGGGCG |
| 19az | SNR52_mCherry_r | 60 | 59.0 | CATGTTATCCTCCTCGCCCTTGCTCACCATCTAGTAGATCATTTATCTTTCACTGCGGAG |
| 19ba | SUP4_mCherry_f | 70 | 59.0 | TCCACCGGCGGCATGGACGAGCTGTACAAGTAATACTAGTTTTTTTGTTTTTTATGTCTTACTAGGGCTG |
| 19bb | hITR_tTPS1_f | 47 | 60.0 | CGCGCTGATCTCTTCTTCGAAACATACTAGGGCTGCAATGACACCAC |
| 19bc | tTPS1_hITR_r | 59 | 60.0 | CCGATGACAGGAAGTGGTGTCATTGCAGCCCTAGTATGTTTCGAAGAAGAGATCAGCGC |
| 19bd | Lbu_pGALL_f | 53 | 59.0 | TAACGTCAAGGAGAAAAAACTATATACTAGATGAAAGTGACGAAGGTAGGAGG |
| 19be | Lbu_tTPS1_r | 70 | 58.0 | ATCGTCTCATTTGCATCGGGTTCACTAGTATTAGTTTTCAGACTTTTTCTCTTCCATTTTGTATTCAAAC |
| 19bf | Lsh_pGALL_f | 48 | 58.0 | TAACGTCAAGGAGAAAAAACTATATACTAGATGGGGAACCTTTTCGGG |
| 19bg | Lsh_tTPS1_r | 64 | 57.0 | ATCGTCTCATTTGCATCGGGTTCACTAGTATTACAGGGTATCGTTTGTATTTTCAATCTTAGTC |
| 19bh | Lwa_pGALL_f | 60 | 59.0 | TAACGTCAAGGAGAAAAAACTATATACTAGATGAAAGTCACTAAAGTCGATGGTATTAGC |
| 19bi | Lwa_tTPS1_r | 48 | 59.0 | ATCGTCTCATTTGCATCGGGTTCACTAGTATTACTGCTCCGCTGTCGC |
| 19bj | CasX_pTXB1_f | 56 | 59.0 | GTTTAACTTTAAGAAGGAGATATACATATGATGGAGAAGCGTATTAACAAGATTCG |
| 19bk | CasX_pTXB1_r | 48 | 60.0 | TAGGGCAACTAGTGCATCTCCCGTGATGCAAGCATTCGGCTTCCAGAC |
| 19bl | CasX_Mut_f | 35 | 58.0 | AATTTTATGCCTGTGAGATTCAATTACAAAAGTGG |
| 19bm | CasX_Mut_r | 31 | 59.0 | CACAGGCATAAAATTCCTTTTCGTCCATCTC |
| 19bn | CmR_mittig_f | 24 | 57.0 | GTTTTCCATGAGCAAACTGAAACG |
| 19bo | CmR_mittig_r | 22 | 59.0 | AGTTTGCTCATGGAAAACGGTG |
| 19bp | Lbu_seq_1 | 27 | 58.0 | TGAGAATAACATCGAGAAGGTTGAAGG |
| 19bq | Lbu_seq_2 | 33 | 58.0 | CAGAACTTGAAAAAATTGATCGAAAATAAGCTG |
| 19br | Lbu_seq_3 | 28 | 58.0 | ATCCGAAATCTCAAAGAAGATGTTTCAG |
| 19bs | Lbu_seq_4 | 23 | 58.0 | TAATGCCGGTAATCAGGACGAAG |
| 19bt | Lbu_seq_5 | 29 | 58.0 | GCAACAAAGTTAAGGATAACAAGGAACTG |
| 19bu | Lsh_seq_1 | 23 | 57.0 | GCGATGAATACACCAACAAGACC |
| 19bv | Lsh_seq_2 | 26 | 59.0 | CAACCGCACGTATATCAAATCATACG |
| 19bw | Lsh_seq_3 | 34 | 57.0 | GTCATAATGATATTGATATGACGACTGTCAATAC |
| 19bx | Lsh_seq_4 | 44 | 58.0 | AATCAGTGAGGAGAATAACAACGATATTAAATATTTACYVGW68 |
| 19by | Lsh_seq_5 | 31 | 58.0 | GACTATCGTAATTAACGACGATTTTGAGTAC |
| 19bz | Lsh_seq_6 | 34 | 59.0 | GGACCAATATATTAAAGACAAGGATCAGGAAATC |
| 19ca | Lwa_seq_1 | 32 | 58.0 | CCAAAAAATCAATGAGAATAATGTGGAAAAGG |
| 19cb | Lwa_seq_2 | 32 | 58.0 | GTATCTTTGAGTACCAAAACCTGAAAAAGTTG |
| 19cc | Lwa_seq_3 | 29 | 58.0 | TTTAAAAATATCGCGCCTTCTGAAATCTC |
| 19cd | Lwa_seq_4 | 22 | 59.0 | GGACAAGGAGGAGAAGAACACG |
| 19ce | Lwa_seq_5 | 28 | 59.0 | GAGAATAAGATCAAAGACCGTAAGGAGC |
| 19cf | p8_mC_L_f | 69 | 58.0 | TCCACCGGCGGCATGGACGAGCTGTACAAGGGTGGTGGGTCAATGAAAAAGTCTTTAGTCCTCAAAGCC |
| 19cg | Mat_Col_f | 20 | 58.0 | ATTGGCTGCAACTGAAACCG |
| 19ch | mC_MatA_f | 43 | 60.0 | CCAGCCGATGTATGGTGGTGGGTCAATGGTGAGCAAGGGCGAG |
| 19ci | pSB_MatA_r | 57 | 59.0 | GTTTCAGTTGCAGCCAATGCCACATCTAGTATTTCTCCTCTTTCTCTAGTATCTCCC |
| 19cj | MatA_GF_f | 80 | 76.0 | GGCATTGGCTGCAACTGAAACCGGGCCAGCCGATGTATGGTGGTGGGTCAATGGTGAGCAAGGGCGAGGAGGATAACATG |
| 19ck | MatA_GF_pSB_r | 80 | 76.0 | CCACCACCATACATCGGCTGGCCCGGTTTCAGTTGCAGCCAATGCCACATCTAGTATTTCTCCTCTTTCTCTAGTATCTC |
| 19cl | MatA_GF_pTX_r | 80 | 76.0 | CCACCACCATACATCGGCTGGCCCGGTTTCAGTTGCAGCCAATGCCACATCATATGTATATCTCCTTCTTAAAGTTAAAC |
| 19cm | pTX_MatA_r | 69 | 57.0 | GTTTCAGTTGCAGCCAATGCCACATCATATGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAG |
| 19cn | Flo_pSB_f | 48 | 60.0 | GAGATACTAGAGAAAGAGGAGAAATACTAGATGTTTCCGACCGCGCTG |
| 19co | Flo_pTX_f | 43 | 60.0 | GTTTAACTTTAAGAAGGAGATATACATATGTTTCCGACCGCGC |
| 19cp | Flo_mCh_r | 50 | 60.0 | CATGTTATCCTCCTCGCCCTTGCTCACCATTGACCCACCACCTTTGGTGC |
| 19cq | Flo_Col_f | 21 | 59.0 | AAGTGTATGCGACCCAGGATG |
| 19cr | mC_pSB_Opy_1_f | 60 | 60.0 | GATAAATGCCCGAGCACCTATTGCGCGAAAGGTGGTGGGTCAATGGTGAGCAAGGGCGAG |
| 19cs | pSB_Opy_1_r | 80 | 59.0 | GCACACCGGGCAGCTCGCGGTGCTATCGCACACCACGCAGCCATCCATCTAGTATTTCTCCTCTTTCTCTAGTATCTCCC |
| 19ct | Opy_2_f | 63 | 62.0 | CGAGCGGCGAATATTGCGTGATGACCAGCCTGACCTGCGATAAATGCCCGAGCACCTATTGCG |
| 19cu | Opy_2_pSB_r | 55 | 63.0 | GCAGGTCAGGCTGGTCATCACGCAATATTCGCCGCTCGCGCACACCGGGCAGCTC |
| 19cv | Opy_2_pTX_r | 67 | 63.0 | GCAGGTCAGGCTGGTCATCACGCAATATTCGCCGCTCGCGCACACCGGGCAGCTCGCGGTGCTATCG |
| 19cw | pTX_Opy_1_r | 80 | 59.0 | GCAGCTCGCGGTGCTATCGCACACCACGCAGCCATCCATCATATGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTC |
| 19cx | mC_pTX_Opy_1_f | 60 | 59.0 | GATAAATGCCCGAGCACCTATTGCGCGAAAGGTGGTGGGTCAGTGAGCAAGGGCGAGGAG |
| 19cy | Opy_Col_f | 22 | 58.0 | GCGAATATTGCGTGATGACCAG |
| 19cz | GALL_seq_f | 40 | 58.0 | CTCTATACTTTAACGTCAAGGAGAAAAAACTATATACTAG |
| 19da | SNR52_seq_f | 40 | 58.0 | TACTAGTCTTTGAAAAGATAATGTATGATTATGCTTTCAC |
| 19db | mCherry_seq1_f | 21 | 60.0 | CCCCGACTACTTGAAGCTGTC |
| 19dc | p8_Col_r | 19 | 59.0 | TTTTGCGGGATCGTCACCC |
| 19dd | mC_f | 17 | 57.0 | ATGGTGAGCAAGGGCGA |
| 19de | M13_PCR_f | 48 | 68.0 | AAGTTTACGAGTAAGGCCAGCTGATAAACCGATACAATTAAAGGCTCC |
| 19df | M13_PCR_r | 47 | 67.0 | TGGCCTTACTCGTAAACTTCTTAAACAGCTTGATACCGATAGTTGCG |
| 19dg | M13_PCR_split_f | 21 | 61.0 | CGCTTCCTCGCTCACTGACTC |
| 19dh | M13_PCR_split_r | 25 | 61.0 | GTGAGCGAGGAAGCGGAATATATCC |
| 19di | M13_pSB3K3_f1 | 49 | 61.0 | AATTCGCGGCCGCTTCTAGAGCGGCCTATTGGTTAAAAAATGAGCTGAT |
| 19dj | M13_pSB3K3_r1 | 39 | 60.0 | CAAAAGGGCGCGTTGAAAATCTCCAAAAAAAAAGGCTCC |
| 19dk | M13_pSB3K3_f2 | 33 | 62.0 | GATTTTCAACGCGCCCTTTTGTCTTTAGCGCTG |
| 19dl | M13_pSB3K3_r2 | 41 | 57.0 | TGCAGCGGCCGCTACTAGTACGCTACAGGGCGCGTACTATG |
| 19dm | pSB3K3_M13_split_f | 20 | 60.0 | TACTAGTAGCGGCCGCTGCA |
| 19dn | pSB3K3_M13_split_r | 21 | 61.0 | CTCTAGAAGCGGCCGCGAATT |
| 19do | Seq_M13_PCR_III-IV_1 | 23 | 60.0 | GGCTGAGACTCCTCAAGAGAAGG |
| 19dp | Seq_M13_PCR_III-IV_2 | 22 | 62.0 | CAACCGATTGAGGGAGGGAAGG |
| 19dq | Seq_M13_PCR_III-IV_3 | 20 | 60.0 | TAGCGAACCTCCCGACTTGC |
| 19dr | Seq_M13_PCR_III-IV_4 | 22 | 59.0 | ATGCTGATGCAAATCCAATCGC |
| 19ds | Seq_M13_PCR_III-IV_5 | 20 | 60.0 | CTCGCGTTCTTTGTCTTGCG |
| 19dt | Seq_M13_PCR_III-IV_6 | 20 | 61.0 | CCGCTCCTTCTGGTGGTTTC |
| 19du | Seq_M13_PCR_III-IV_7 | 20 | 58.0 | CCAGCAAGGCCGATAGTTTG |
| 19dv | Seq_M13_PCR_II-VIII_1 | 23 | 59.0 | CTGGTCTGTACACCGTTCATCTG |
| 19dw | Seq_M13_PCR_II-VIII_2 | 20 | 60.0 | CTGGTTCGCTTTGAAGCTCG |
| 19dx | Seq_M13_PCR_II-VIII_3 | 21 | 60.0 | GTTTGCTCCAGACTCTCAGGC |
| 19dy | Seq_M13_PCR_II-VIII_4 | 21 | 60.0 | CCTGGAATGCTGTTTTCCCGG |
| 19dz | Seq_M13_PCR_II-VIII_5 | 21 | 60.0 | GCATGGTTACTCACCACTGCG |
| 19ea | Seq_M13_PCR_II-VIII_6 | 19 | 61.0 | GACCGTTCCGTGGCAAAGC |
| 19eb | Seq_M13_PCR_II-VIII_7 | 20 | 60.0 | GTTACCGGATAAGGCGCAGC |
| 19ec | Seq_M13_PCR_GA_III | 26 | 60.0 | CAACCCTCATAGTTAGCGTAACGATC |
| 19ed | pT7_seq_f | 30 | 59.0 | TAATACGACTCACTATAGGGGAATTGTGAG |
| 19ee | CasX_seq1_f | 24 | 59.0 | CCGCTGTTCGTATATAAGCTGGAG |
| 19ef | CasX_seq2_f | 27 | 59.0 | TGGGTAAATTTGAATCTGTGGCAGAAG |
| 19eg | CasX_seq3_f | 29 | 59.0 | CTTTGTTCTTGAGCGTTTAAAAGAGATGG |
| 19eh | CasX_seq4_f | 16 | 60.0 | CGATGAGCCGGCCCTG |
| 19ei | CasX_seq5_f | 26 | 58.0 | CGTATTTGTCGAAAACTCTTGCTCAG |
| 19ej | Intein_seq_r | 17 | 58.0 | CACGATGTCGGCGATGC |
| 19ek | pSB1C3ori_seq_r | 18 | 59.0 | TGGGCTGTGTGCACGAAC |
| 19el | Lbu_Xeco_f | 51 | 66.0 | TATGAGATCAATGAATTTCTGCGTGAGATCAAACTGGGAAACATCCTGAAG |
| 19em | Lbu_Xeco_r | 54 | 65.0 | TCACGCAGAAATTCATTGATCTCATAGGGGATCTTGATATTATTGTTCTGCTCG |
| 19en | Lbu_Xpst_f | 53 | 67.0 | GAATTTAATGAGCTGAATTTACTGCAAGGCCTGTTGCTGCGCATTTTACATCG |
| 19eo | Lbu_Xpst_r | 57 | 66.0 | TTGCAGTAAATTCAGCTCATTAAATTCTACCTTGTTCTTAAGGTGGGTGTACTCCTC |
| 19ep | SUP4_guideRNA_f | 37 | 59.0 | TACTAGTTTTTTTGTTTTTTATGTCTTACTAGGGCTG |
| 19eq | SNR_guideRNA_r | 30 | 58.0 | CTAGTAGATCATTTATCTTTCACTGCGGAG |
| 19er | pSB1C3_GG_r | 29 | 60.0 | GAAGACGAGAAGCTCTAGAAGCGGCCGCG |
| 19es | pSB1C3_GG_f | 31 | 59.0 | GAAGACCACCACTACTAGTAGCGGCCGCTGC |
| 19et | pSB1C3_GG2_f | 31 | 61.0 | GAAGACACCCCATACTAGTAGCGGCCGCTGC |
| 19eu | pSB1C3_GG2_r | 36 | 61.0 | GAAGACTTTGGGGTAGTCTAAATCGCATCCTGTTTG |
| 19ev | GG_1_r | 74 | nan | AATGTTTTAGTCCCCTTCGTTTTTGGGGTAGTCTAAATCCTAGTACTAGTAGATCATTTATCTTTCACTGCGGA |
| 19ew | GG_2_r | 74 | nan | CCGCAATTAAACTTGGGCTCGTTTTAGTCCCCTTCGTTTTTGGGGTAGTCTAAATCATGAACGCTTAGCGACGA |
| 19ex | GG_3_r | 74 | nan | TGGGGTAGTCTAAATCGCATCCTGTTTGACGACACCCGTTTTAGTCCCCTTCGTTTTTGGGGTAGTCTAAATCT |
| 19ey | GG_4_r | 74 | nan | TTAGTCCCCTTCGTTTTTGGGGTAGTCTAAATCGCAGCGATACTTTCTCAGCAGGTTTTAGTCCCCTTCGTTTT |
| 19ez | GG_5_r | 74 | nan | CGAACCGCCAGAATGTTTTAGTCCCCTTCGTTTTTGGGGTAGTCTAAATCAGTCCCTACGTCAAACCCTCTGTT |
| 19fa | GG_6_r | 74 | nan | AGTCTAAATCCAGTTTCATCGATTCCTATTCGTTTTAGTCCCCTTCGTTTTTGGGGTAGTCTAAATCTGTACGA |
| 19fb | GG_7_r | 75 | nan | GTGGTGTCATTGCAGCCCTAGTAAGACATAAAAAACAAAAAAACTAGTAGTTTTAGTCCCCTTCGTTTTTGGGGT |
| 19fc | GG_1_f | 74 | nan | CTTCTCCGCAGTGAAAGATAAATGATCTACTAGTACTAGGATTTAGACTACCCCAAAAACGAAGGGGACTAAAA |
| 19fd | GG_2_f | 74 | nan | CATTTCGTCGCTAAGCGTTCATGATTTAGACTACCCCAAAAACGAAGGGGACTAAAACGAGCCCAAGTTTAATT |
| 19fe | GG_3_f | 74 | nan | GCGGAGATTTAGACTACCCCAAAAACGAAGGGGACTAAAACGGGTGTCGTCAAACAGGATGCGATTTAGACTAC |
| 19ff | GG_4_f | 74 | nan | CCCAAAAACGAAGGGGACTAAAACCTGCTGAGAAAGTATCGCTGCGATTTAGACTACCCCAAAAACGAAGGGGA |
| 19fg | GG_5_f | 74 | nan | CTAAAACAGAGGGTTTGACGTAGGGACTGATTTAGACTACCCCAAAAACGAAGGGGACTAAAACATTCTGGCGG |
| 19fh | GG_6_f | 74 | nan | TTCGTCGTACAGATTTAGACTACCCCAAAAACGAAGGGGACTAAAACGAATAGGAATCGATGAAACTGGATTTA |
| 19fi | GG_7_f | 75 | nan | GACTACCCCAAAAACGAAGGGGACTAAAACTACTAGTTTTTTTGTTTTTTATGTCTTACTAGGGCTGCAATGACA |
| 19fj | Gib_gRNA_f | 57 | 58.0 | AAGTTGGTGCGCATGTTTCGGCGTTCGAAACTTCTCCGCAGTGAAAGATAAATGATC |
| 19fk | Gib_gRNA_r | 51 | 60.0 | AACCTTTTACGGGCACACCGATGACAGGAAGTGGTGTCATTGCAGCCCTAG |
| 19fl | FAP7_r | 21 | 60.0 | GCATCCTGTTTGACGACACCC |
| 19fm | SWP1_r | 29 | 59.0 | CTAAATCCAGTTTCATCGATTCCTATTCG |
| 19fn | pSB1C3_In_seq_f | 22 | 58.0 | GGATGATTTCTGGAATTCGCGG |
| 19fo | pSB1C3_In_seq_r | 20 | 59.0 | CCTTTTTTGCCGGACTGCAG |
| 19fp | f1ori_pSB1C3_f | 38 | 59.0 | CGCGGCCGCTTCTAGAGGCTACTGCGTCACGGATCCCC |
| 19fq | f1ori_mC_Prom_r | 71 | 62.0 | GACCCCCAGCGATTATACCAAGCGCGAAACAAAGTACAACGGAGATTCATTTCCCCGAAAAGTGCCACCTG |
| 19fr | mC_f1ori_RBS_f | 61 | 59.0 | GCTTGGTATAATCGCTGGGGGTCAAAGTAATGGAAACTTCCTCATGGTGAGCAAGGGCGAG |
| 19fs | mC_pSB1C3_r | 44 | 61.0 | TGCAGCGGCCGCTACTAGTACTACTTGTACAGCTCGTCCATGCC |
| 19ft | mC_VIII_r | 48 | 59.0 | CGCTACCGCTACCGCTGCCAGAGCCCTTGTACAGCTCGTCCATGCCGC |
| 19fu | VIII_mC_f | 56 | 62.0 | TGGCAGCGGTAGCGGTAGCGGCAGCATGAAAAAGTCTTTAGTCCTCAAAGCCTCTG |
| 19fv | VIII_III_r | 48 | 60.0 | CTTTTCATAATCAAAATCCACGTTGAAAATCTCCAAAAAAAAAGGCTC |
| 19fw | III_VIII_f | 53 | 61.0 | GGAGATTTTCAACGTGGATTTTGATTATGAAAAGATGGCAAACGCTAATAAGG |
| 19fx | III_pSB1C3_r | 52 | 59.0 | TGCAGCGGCCGCTACTAGTAGATTAAGACTCCTTATTACGCAGTATGTTAGC |
| 19fy | Opy_2_pTX_r_2 | 74 | 63.0 | ATTTATCGCAGGTCAGGCTGGTCATCACGCAATATTCGCCGCTCGCGCACACCGGGCAGCTCGCGGTGCTATCG |
| 19fz | Opy_2_pSB_r_2 | 62 | 63.0 | ATTTATCGCAGGTCAGGCTGGTCATCACGCAATATTCGCCGCTCGCGCACACCGGGCAGCTC |
| 19ga | Opy_2_f_2 | 38 | 62.0 | CAGCCTGACCTGCGATAAATGCCCGAGCACCTATTGCG |
| 19gb | Seq_pSB3K3_1_R | 24 | 59.0 | GAAGGTTCTCTGAGCTACCAACTC |
| 19gc | Seq_pSB3K3_2_R | 22 | 60.0 | GCAGCCTTTTTTCTCCTGCCAC |
| 19gd | Seq_pSB3K3_3_R | 23 | 59.0 | CGGAATTTATGCCTCTTCCGACC |
| 19ge | Seq_pSB3K3_4_R | 25 | 60.0 | GGTTGTATTGATGTTGGACGAGTCG |
| 19gf | Seq_pSB3K3_5_F | 23 | 59.0 | GTGCCACCTGACGTCTAAGAAAC |
| 19gg | M13_Prom_GA_R | 53 | 56.0 | ATCAGCTGGCCTTACTCGTAAACTTCTTAAACAGCTTGATACCGATAGTTGCG |
| 19gh | M13_Term_GA_F | 53 | 59.0 | TTAAGAAGTTTACGAGTAAGGCCAGCTGATAAACCGATACAATTAAAGGCTCC |
| 19gi | m13_Term_GA_R | 40 | 60.0 | ACAAAAGGGCGCGTTGAAAATCTCCAAAAAAAAAGGCTCC |
| 19gj | Term_pSB3K3_GA_R | 49 | 61.0 | TGCAGCGGCCGCTACTAGTACGTTGAAAATCTCCAAAAAAAAAGGCTCC |
| 19gk | VIII_mC_OP_F | 62 | 62.0 | GGCTCTGGCAGCGGTAGCGGTAGCGGCAGCCATGAAAAAGTCTTTAGTCCTCAAAGCCTCTG |
| 19gl | VIII_III_OP_R | 50 | 60.0 | ATCTTTTCATAATCAAAATCCACGTTGAAAATCTCCAAAAAAAAAGGCTC |
| 19gm | III_VIII_OP_F | 56 | 61.0 | TTTGGAGATTTTCAACGTGGATTTTGATTATGAAAAGATGGCAAACGCTAATAAGG |
| 19gn | III_1C3_OP_R | 68 | 59.0 | CCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTAGATTAAGACTCCTTATTACGCAGTATGTTAGC |
| 19go | f1_1C3_OP_F | 56 | 60.0 | AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGGCTACTGCGTCACGGATCCCC |
| 19gp | f1_mC_Prom_OP_R | 74 | 64.0 | TTGACCCCCAGCGATTATACCAAGCGCGAAACAAAGTACAACGGAGATTACATTTCCCCGAAAAGTGCCACCTG |
| 19gq | mC_ori_OP_F | 66 | 60.0 | TTCGCGCTTGGTATAATCGCTGGGGGTCAAAGTAATGGAAACTTCCTCATGGTGAGCAAGGGCGAG |
| 19gr | mC_VIII_OP_R | 61 | 63.0 | TTTTCATGGCTGCCGCTACCGCTACCGCTGCCAGAGCCCTTGTACAGCTCGTCCATGCCGC |
| 19gs | Alle7_gRNA_1_f | 80 | nan | CCGCAGTGAAAGATAAATGATCTACTAGTACTAGGATTTAGACTACCCCAAAAACGAAGGGGACTAAAACATTTCGTCGC |
| 19gt | Alle7_gRNA_1_r | 80 | nan | AAATCATGAACGCTTAGCGACGAAATGTTTTAGTCCCCTTCGTTTTTGGGGTAGTCTAAATCCTAGTACTAGTAGATCAT |
| 19gu | Alle7_gRNA_2_f | 60 | nan | TAAGCGTTCATGATTTAGACTACCCCAAAAACGAAGGGGACTAAAACGAGCCCAAGTTTA |
| 19gv | Alle7_gRNA_2_r | 60 | nan | TCTAAATCTCCGCAATTAAACTTGGGCTCGTTTTAGTCCCCTTCGTTTTTGGGGTAGTCT |
| 19gw | Alle7_gRNA_3_f | 60 | nan | ATTGCGGAGATTTAGACTACCCCAAAAACGAAGGGGACTAAAACGGGTGTCGTCAAACAG |
| 19gx | Alle7_gRNA_3_r | 60 | nan | TAGTCTAAATCGCATCCTGTTTGACGACACCCGTTTTAGTCCCCTTCGTTTTTGGGGTAG |
| 19gy | Alle7_gRNA_4_f | 80 | nan | GATGCGATTTAGACTACCCCAAAAACGAAGGGGACTAAAACCTGCTGAGAAAGTATCGCTGCGATTTAGACTACCCCAAA |
| 19gz | Alle7_gRNA_4_r | 80 | nan | TCTGTTTTAGTCCCCTTCGTTTTTGGGGTAGTCTAAATCGCAGCGATACTTTCTCAGCAGGTTTTAGTCCCCTTCGTTTT |
| 19ha | Alle7_gRNA_5_f | 80 | nan | AGGGGACTAAAACAGAGGGTTTGACGTAGGGACTGATTTAGACTACCCCAAAAACGAAGGGGACTAAAACATTCTGGCGG |
| 19hb | Alle7_gRNA_5_r | 80 | nan | AAATCTGTACGACGAACCGCCAGAATGTTTTAGTCCCCTTCGTTTTTGGGGTAGTCTAAATCAGTCCCTACGTCAAACCC |
| 19hc | Alle7_gRNA_6_f | 60 | nan | TTCGTCGTACAGATTTAGACTACCCCAAAAACGAAGGGGACTAAAACGAATAGGAATCGA |
| 19hd | Alle7_gRNA_6_r | 60 | nan | TCTAAATCCAGTTTCATCGATTCCTATTCGTTTTAGTCCCCTTCGTTTTTGGGGTAGTCT |
| 19he | Alle7_gRAN_7_f | 80 | nan | TGAAACTGGATTTAGACTACCCCAAAAACGAAGGGGACTAAAACTACTAGTTTTTTTGTTTTTTATGTCTTACTAGGGCT |
| 19hf | Alle7_gRNA_7_r | 77 | nan | GTGGTGTCATTGCAGCCCTAGTAAGACATAAAAAACAAAAAAACTAGTAGTTTTAGTCCCCTTCGTTTTTGGGGTAG |
| 19hg | TPS1_seq_r | 18 | 59.0 | GGACGTGTGGCGTTTCAG |
| 19hh | GG_BB_1_f | 31 | 61.0 | GAAGACATCATTTACTAGTAGCGGCCGCTGC |
| 19hi | GG_BB_2_f | 31 | 59.0 | GAAGACATGCGGTACTAGTAGCGGCCGCTGC |
| 19hj | GG_BB_2_r | 35 | 60.0 | GAAGACATAATGTTTTAGTCCCCTTCGTTTTTGGG |
| 19hk | GG_BB_3_f | 31 | 60.0 | GAAGACATCCCATACTAGTAGCGGCCGCTGC |
| 19hl | GG_BB_3_r | 29 | 61.0 | GAAGACATCCGCAATTAAACTTGGGCTCG |
| 19hm | Gib_gRNA_1_3_r | 45 | 60.0 | GGTTTTAGTCCCCTTCGTTTTTGGGGTAGTCTAAATCGCATCCTG |
| 19hn | GG2_BB_4_f | 31 | 59.0 | GAAGACACCTAATACTAGTAGCGGCCGCTGC |
| 19ho | GG2_BB_4_r | 29 | 61.0 | GAAGACATTGGGCTCTAGAAGCGGCCGCG |
| 19hp | GG2_BB_5_f | 31 | 56.0 | GAAGACACTTCGTACTAGTAGCGGCCGCTGC |
| 19hq | GG_BB_5_r | 33 | 61.0 | GAAGACATTTAGTCCCCTTCGTTTTTGGGGTAG |
| 19hr | GG_BB_6_f | 31 | 57.0 | GAAGACACGACTTACTAGTAGCGGCCGCTGC |
| 19hs | GG_BB_6_r | 32 | 60.0 | GAAGACATCGAACCGCCAGAATGTTTTAGTCC |
| 19ht | GG_BB_7_f | 31 | 61.0 | GAAGACACCCACTACTAGTAGCGGCCGCTGC |
| 19hu | GG_BB_7_r | 40 | 60.0 | GAAGACATAGTCTAAATCCAGTTTCATCGATTCCTATTCG |
| 19hv | Gib_gRNA_4_7_f | 45 | 60.0 | CAGGATGCGATTTAGACTACCCCAAAAACGAAGGGGACTAAAACC |
| 19hw | pSB1C3_J23100_f | 64 | 60.0 | GCTAGCTCAGTCCTAGGTACAGTGCTAGCTACTAGAGAAAGAGGAGAAATACTAGATGGTGAGC |
| 19hx | pSB1C3_J23100_r | 48 | 60.0 | GTACCTAGGACTGAGCTAGCCGTCAACTCTAGAAGCGGCCGCGAATTC |
| 19hy | pSB1C3_J23104_f | 65 | 64.0 | GCTCAGTCCTAGGTATTGTGCTAGCTACTAGAGAAAGAGGAGAAATACTAGATGGTGAGCAAGGG |
| 19hz | pSB1C3_J23104_r | 53 | 64.0 | GCACAATACCTAGGACTGAGCTAGCTGTCAACTCTAGAAGCGGCCGCGAATTC |
| 19ia | pSB1C3_J23108_f | 63 | 60.0 | CTAGCTCAGTCCTAGGTATAATGCTAGCTACTAGAGAAAGAGGAGAAATACTAGATGGTGAGC |
| 19ib | pSB1C3_J23108_r | 53 | 62.0 | GCATTATACCTAGGACTGAGCTAGCTGTCAGCTCTAGAAGCGGCCGCGAATTC |
| 19ic | pSB1C3_J23110_f | 63 | 60.0 | CTAGCTCAGTCCTAGGTACAATGCTAGCTACTAGAGAAAGAGGAGAAATACTAGATGGTGAGC |
| 19id | pSB1C3_J23110_r | 52 | 60.0 | CATTGTACCTAGGACTGAGCTAGCCGTAAACTCTAGAAGCGGCCGCGAATTC |
| 19ie | pSB1C3_J23114_f | 64 | 60.0 | GCTAGCTCAGTCCTAGGTACAATGCTAGCTACTAGAGAAAGAGGAGAAATACTAGATGGTGAGC |
| 19if | pSB1C3_J23114_r | 52 | 64.0 | CATTGTACCTAGGACTGAGCTAGCCATAAACTCTAGAAGCGGCCGCGAATTC |
| 19ig | Gib_Opy_Fus_f | 48 | 60.0 | GTTTAACTTTAAGAAGGAGATATACATATGATGGATGGCTGCGTGGTG |
| 19ih | Gib_Opy_Fus_r | 55 | 59.0 | GGGTAGGGCAACTAGTGCATCTCCCGTGATGCAGCTTGCTTTCGAGGTGAATTTC |
| 19ii | Col_ATP16_r | 22 | 59.0 | AAATCATGAACGCTTAGCGACG |
| 19ij | Col_BRR6_r | 21 | 58.0 | TCCGCAATTAAACTTGGGCTC |
| 19ik | Col_LCP5_r | 21 | 58.0 | GCAGCGATACTTTCTCAGCAG |
| 19il | Col_RQN1_r | 21 | 60.0 | CAGTCCCTACGTCAAACCCTC |
| 19im | Col_SLA2_r | 18 | 60.0 | TGTACGACGAACCGCCAG |
| 19in | pSB1C3_P8_f | 68 | 62.0 | GTTTCGCGCTTGGTATAATCGCTGGGGGTCTACTAGAGAAAGAGGAGAAATACTAGATGGTGAGCAAG |
| 19io | mCherry | 60 | 62.0 | CGATTATACCAAGCGCGAAACAAAGTACAACGGAGATTCTCTAGAAGCGGCCGCGAATTC |
| 19ip | Bba_J23100_col | 24 | 59.0 | GCTAGCACTGTACCTAGGACTGAG |
| 19iq | Bba_J23104_col | 25 | 60.0 | GCTAGCACAATACCTAGGACTGAGC |
| 19ir | Bba_J23108_col | 28 | 59.0 | GCTAGCATTATACCTAGGACTGAGCTAG |
| 19is | Bba_J23110_col | 25 | 60.0 | GCTAGCATTGTACCTAGGACTGAGC |
| 19it | P8_Prom_col | 23 | 61.0 | GACCCCCAGCGATTATACCAAGC |
| 19iu | SC_ITR_f | 25 | 58.0 | CAATTGGGACGTATGATTGTTGAGG |
| 19iv | SC_ITR_r | 21 | 58.0 | AAGAACCTTTTACGGGCACAC |
| 19iw | NotI_BI_pRS_f | 29 | 62.0 | ATCTGCGGCCGCCGACAGCCCTCCGACGG |
| 19ix | BamHI_BI_pRS_r | 35 | 60.0 | ATCTGGATCCGGTGTCATTGCAGCCCTAGTAAGAC |
| 19iy | Lwa_pTXB1_f | 59 | 57.0 | GTTTAACTTTAAGAAGGAGATATACATATGATGAAAGTCACTAAAGTCGATGGTATTAG |
| 19iz | Lwa_pTXB1_r | 45 | 61.0 | TAGGGCAACTAGTGCATCTCCCGTGATGCACTGCTCCGCTGTCGC |
| 19ja | Lsh_pTXB1_f | 48 | 57.0 | GTTTAACTTTAAGAAGGAGATATACATATGATGGGGAACCTTTTCGGG |
| 19jb | Lsh_pTXB1_r | 61 | 59.0 | TAGGGCAACTAGTGCATCTCCCGTGATGCACAGGGTATCGTTTGTATTTTCAATCTTAGTC |
| 19jc | Lbu_pTXB1_f | 53 | 58.0 | GTTTAACTTTAAGAAGGAGATATACATATGATGAAAGTGACGAAGGTAGGAGG |
| 19jd | Lbu_pTXB1_r | 63 | 59.0 | TAGGGCAACTAGTGCATCTCCCGTGATGCAGTTTTCAGACTTTTTCTCTTCCATTTTGTATTC |
| 19je | RFP_f | 19 | 60.0 | ATGGCTTCCTCCGAAGACG |
| 19jf | RFP_r | 20 | 58.0 | TTAAGCACCGGTGGAGTGAC |
| 19jg | T7Te_f | 17 | 59.0 | GGCTCACCTTCGGGTGG |
| 19jh | prefix_T7_r | 66 | 60.0 | CTAGTATTTCTCCTCTTTCTCTAGTATCTCCCTATAGTGAGTCGTATTACTCTAGAAGCGGCCGCG |
| 19ji | Lbu_RBS_f | 53 | 58.0 | GAGATACTAGAGAAAGAGGAGAAATACTAGATGAAAGTGACGAAGGTAGGAGG |
| 19jj | Lsh_RBS_f | 48 | 57.0 | GAGATACTAGAGAAAGAGGAGAAATACTAGATGGGGAACCTTTTCGGG |
| 19jk | Lwa_RBS_f | 60 | 59.0 | GAGATACTAGAGAAAGAGGAGAAATACTAGATGAAAGTCACTAAAGTCGATGGTATTAGC |
| 19jl | CasX_RBS_f | 56 | 58.0 | GAGATACTAGAGAAAGAGGAGAAATACTAGATGGAGAAGCGTATTAACAAGATTCG |
| 19jm | Lbu_T7Te_r | 72 | 59.0 | AACGCAGAAAGGCCCACCCGAAGGTGAGCCCTAGTATTAGTTTTCAGACTTTTTCTCTTCCATTTTGTATTC |
| 19jn | Lsh_T7Te_r | 70 | 59.0 | AACGCAGAAAGGCCCACCCGAAGGTGAGCCCTAGTATTACAGGGTATCGTTTGTATTTTCAATCTTAGTC |
| 19jo | Lwa_T7Te_r | 54 | 62.0 | AACGCAGAAAGGCCCACCCGAAGGTGAGCCCTAGTATTACTGCTCCGCTGTCGC |
| 19jp | CasX_T7Te_r | 58 | 61.0 | AACGCAGAAAGGCCCACCCGAAGGTGAGCCCTAGTATTAAGCATTCGGCTTCCAGACC |
| 19jq | gRNA_RFP_f | 80 | 70.0 | TTTTAGATGAAGATGATTTAGACTACCCCAAAAACGAAGGGGACTAAAACCAAAGCTTACGTTAAACACCCGATTTAGAC |
| 19jr | gRNA_RFP_r | 80 | 70.0 | AATCGGGTGTTTAACGTAAGCTTTGGTTTTAGTCCCCTTCGTTTTTGGGGTAGTCTAAATCATCTTCATCTAAAATATAC |
| 19js | Lsh_gRNA_r | 80 | 60.0 | TTTTTGGGGTAGTCTAAATCATCTTCATCTAAAATATACTCTAGTATTACAGGGTATCGTTTGTATTTTCAATCTTAGTC |
| 19jt | Lwa_gRNA_r | 80 | 61.0 | GTTTTAGTCCCCTTCGTTTTTGGGGTAGTCTAAATCATCTTCATCTAAAATATACTCTAGTATTACTGCTCCGCTGTCGC |
| 19ju | Lbu_gRNA_r | 80 | 59.0 | TTTGGGGTAGTCTAAATCATCTTCATCTAAAATATACTCTAGTATTAGTTTTCAGACTTTTTCTCTTCCATTTTGTATTC |
| 19jv | T7Te_gRNA_f | 80 | 61.0 | CAAAGCTTACGTTAAACACCCGATTTAGACTACCCCAAAAACGAAGGGGACTAAAACTACTAGGGCTCACCTTCGGGTGG |
| 19jw | CasX_gRNA_r | 80 | 59.0 | CCATACGACATAGTCGCTGGGACTGGCTCCAAATCTTCATCTAAAATATACTCTAGTATTAAGCATTCGGCTTCCAGACC |
| 19jx | T7Te_gRNAX_f | 80 | 61.0 | GTAAAACGCATCAAAGTCCTGCAGCAGAAAATCAAACAAAGCTTACGTTAAACACCCTACTAGGGCTCACCTTCGGGTGG |
| 19jy | gRNAX_RFP_f | 80 | 72.0 | CTATGTCGTATGGACGAAGCGCTTATTTATCGGAGAGAAACCGATAAGTAAAACGCATCAAAGTCCTGCAGCAGAAAATC |
| 19jz | gRNAX_RFP_r | 80 | 74.0 | CTTTGATGCGTTTTACTTATCGGTTTCTCTCCGATAAATAAGCGCTTCGTCCATACGACATAGTCGCTGGGACTGGCTCC |
| 19ka | II_3K3_f | 58 | 61.0 | GATTTCTGGAATTCGCGGCCGCTTCTAGAGCGGCCTATTGGTTAAAAAATGAGCTGAT |
| 19kb | Term_VIII_f | 61 | 53.0 | TTAAGAAGTTTACGAGTAAGGCCAGCTGATTACTAGAGAAACCGATACAATTAAAGGCTCC |
| 19kc | VIII_Term_r | 55 | 58.0 | ATCAGCTGGCCTTACTCGTAAACTTCTTAAACAGCTTGATACCGATAGTTGCGCC |
| 19kd | Term_III_r | 46 | 58.0 | ATTCAACCGACTCTAGTAGTTGAAAATCTCCAAAAAAAAAGGCTCC |
| 19ke | III_Term_f | 40 | 59.0 | GATTTTCAACTACTAGAGTCGGTTGAATGTCGCCCTTTTG |
| 19kf | IV_3K3_r | 51 | 57.0 | TTTGCCGGACTGCAGCGGCCGCTACTAGTACGCTACAGGGCGCGTACTATG |
| 19kg | VF2 primer | 20 | 60.0 | TGCCACCTGACGTCTAAGAA |
| 19kh | f1_1C3_f | 51 | 76.0 | GATTTCTGGAATTCGCGGCCGCTTCTAGAGGCTACTGCGTCACGGATCCCC |
| 19ki | mC_RBS_Prom_f | 80 | 76.0 | CTCCGTTGTACTTTGTTTCGCGCTTGGTATAATCGCTGGGGGTCAAAGTAATGGAAACTTCCTCATGGTGAGCAAGGGCG |
| 19kj | f1_Prom_r | 60 | 73.0 | ATACCAAGCGCGAAACAAAGTACAACGGAGATTCTCTAGTATTCCCCGAAAAGTGCCACC |
| 19kk | VIII_mC_OP_f2 | 61 | 76.0 | GGCTCTGGCAGCGGTAGCGGTAGCGGCAGCATGAAAAAGTCTTTAGTCCTCAAAGCCTCTG |
| 19kl | mC_VIII_OP_r2 | 60 | 80.0 | TTTTCATGCTGCCGCTACCGCTACCGCTGCCAGAGCCCTTGTACAGCTCGTCCATGCCGC |
| 19km | III_VIII_f | 50 | 66.0 | TTTTCAACGTGTACTAGAGATGGATTTTGATTATGAAAAGATGGCAAACG |
| 19kn | VIII_III_r | 52 | 65.0 | TAATCAAAATCCATCTCTAGTACACGTTGAAAATCTCCAAAAAAAAAGGCTC |
| 19ko | III_1C3_r | 60 | 73.0 | TTTGCCGGACTGCAGCGGCCGCTACTAGTATTAAGACTCCTTATTACGCAGTATGTTAGC |
| 19kp | mC_f1_N_f | 42 | 67.0 | GGGGAAATGTACTAGAGAATCTCCGTTGTACTTTGTTTCGCG |
| 19kq | f1_mC_N_r | 39 | 68.0 | ACGGAGATTCTCTAGTACATTTCCCCGAAAAGTGCCACC |
| 19kr | III_VIII_N_f | 49 | 66.0 | ATTTTCAACTACTAGAGGTGGATTTTGATTATGAAAAGATGGCAAACGC |
| 19ks | VIII_III_N_r | 45 | 65.0 | AAAATCCACCTCTAGTAGTTGAAAATCTCCAAAAAAAAAGGCTCC |
| 19kt | VIII_mC_f_1.8. | 46 | 65.0 | AGGCGGCAGCATGAAAAAGTCTTTAGTCCTCAAAGCCTCTGTAGCC |
| 19ku | mC_VIII_r_1.8. | 37 | 64.0 | ACTAAAGACTTTTTCATGCTGCCGCTACCGCTACCGC |
| 19kv | pRS304_f | 18 | 57.0 | AAGTTGGGTAACGCCGTG |
| 19kw | pRS304_r | 20 | 58.0 | CCATGATTACGCCAAGCTCG |
| 19kx | VIII_uTerm_R | 64 | 66.0 | TCCAAAAGGAGCCTTTAATTGTATCGGTTTCTCTAGTAATCAGCTTGCTTTCGAGGTGAATTTC |
| 19ky | III_oTerm_F | 60 | 59.0 | TTAAGAAGTTTACGAGTAAGGCCAGCTGATTACTAGAGTCGGTTGAATGTCGCCCTTTTG |
| 19kz | III_uTerm_F | 80 | 59.0 | GATACAATTAAAGGCTCCTTTTGGAGCCTTTTTTTTTGGAGATTTTCAACTACTAGAGTCGGTTGAATGTCGCCCTTTTG |
| 19la | mC_cmyc_R | 51 | 60.0 | CAAGTCCTCTTCAGAAATAAGCTTTTGTTCCTTGTACAGCTCGTCCATGCC |
| 19lb | VIII_cmyc_F | 61 | 61.0 | GAACAAAAGCTTATTTCTGAAGAGGACTTGATGAAAAAGTCTTTAGTCCTCAAAGCCTCTG |
| 19lc | IV_3K3_Hp_r | 64 | 65.0 | CTGCAGCGGCCGCTACTAGTATCACAGGGCGCGAACGATGGTTGCTTTGACGAGCACGTATAAC |
| 19ld | III_Term_Hp_f | 51 | 63.0 | GATTTTCAACTACTAGAGATGTCGGTTGAATGTCGCCCTTTTGTCTTTAGC |
| 19le | III_oTerm_Hp_f | 68 | 63.0 | TTAAGAAGTTTACGAGTAAGGCCAGCTGATTACTAGAGTCGGTTGAATGTCGCCCTTTTGTCTTTAGC |
| 19lf | 3K3_Hp_Split_f | 39 | 61.0 | ATCGTTCGCGCCCTGTGATACTAGTAGCGGCCGCTGCAG |
| 19lg | gRNA3_bI_r | 51 | 60.0 | AGTAAGACATAAAAAACAAAAAAACTAGTATGGGGTAGTCTAAATCGCATC |
| 19lh | pRS304_bI_r | 15 | 60.0 | GCCACCGCGGTGGAG |
| 19li | pRS304_bI_f | 15 | 59.0 | GATCCCCCGGGCTGC |
| 19lj | bI_pRS304_f | 48 | 58.0 | TAGGGCGAATTGGAGCTCCACCGCGGTGGCTACTAGCGACAGCCCTCC |
| 19lk | bI_pRS304_r | 79 | 59.0 | TGATATCGAATTCCTGCAGCCCGGGGGATCCTAGTAAGACATAAAAAACAAAAAAACTAGTACTAGTAGATCATTTATC |
| 19ll | VR | 20 | 60.0 | ATTACCGCCTTTGAGTGAGC |
| 19lm | S5_rRNA1_f | 75 | 80.0 | CACATACGACCACAGGGTGTGGAAAACAGGGCTTCCCGTCCGCTCAGCCGTACTTAAGCCACACGCCGGGAGGTT |
| 19ln | S5_rRNA1_r | 75 | 79.0 | TACTAACCTCCCGGCGTGTGGCTTAAGTACGGCTGAGCGGACGGGAAGCCCTGTTTTCCACACCCTGTGGTCGTA |
| 19lo | S5_rRNA2_f | 52 | 70.0 | AGTAGTTGGGTGGGTGACCACCAGCGAATCCCTTCTGTTGTATGTACTAGTA |
| 19lp | S5_rRNA2_r | 60 | 71.0 | GAAGACTCCTAGTACTAGTACATACAACAGAAGGGATTCGCTGGTGGTCACCCACCCAAC |
| 19lq | hemF_1105_f | 70 | 73.0 | CTAGGATTTAGACTACCCCAAAAACGAAGGGGACTAAAACCGAATTCCCCGGGTGTCAAGCGATTTAGAC |
| 19lr | hemF_1105_r | 70 | 73.0 | GGTAGTCTAAATCGCTTGACACCCGGGGAATTCGGTTTTAGTCCCCTTCGTTTTTGGGGTAGTCTAAATC |
| 19ls | secA_608_f | 65 | 73.0 | TACCCCAAAAACGAAGGGGACTAAAACTGGCGCGGTTCATCCAGAATGGATTTAGACTACCCCAA |
| 19lt | secA_608_r | 65 | 72.0 | GTTTTTGGGGTAGTCTAAATCCATTCTGGATGAACCGCGCCAGTTTTAGTCCCCTTCGTTTTTGG |
| 19lu | hemF_49_f | 74 | 71.0 | GACTAAAACTTCTCATCTCGGACCTGTCACGATTTAGACTACCCCAAAAACGAAGGGGACTAAAACTACTAGTT |
| 19lv | hemF_49_r | 83 | 72.0 | AAAAAACTAGTAGTTTTAGTCCCCTTCGTTTTTGGGGTAGTCTAAATCGTGACAGGTCCGAGATGAGAAGTTTTAGTCCCCTT |
| 19lw | BB_1GG_f | 37 | 60.0 | GAAGACGCTACCTTTTTTTACTAGTAGCGGCCGCTGC |
| 19lx | BB_2GG_f | 31 | 61.0 | GAAGACGCAAACTACTAGTAGCGGCCGCTGC |
| 19ly | BB_3GG_f | 31 | 61.0 | GAAGACACTTTTTACTAGTAGCGGCCGCTGC |
| 19lz | BB_S5_r | 29 | 60.0 | GAAGACAGTGTGCTCTAGAAGCGGCCGCG |
| 19ma | BB_1GG_r | 39 | 59.0 | GAAGACTCCTAGTACTAGTACATACAACAGAAGGGATTC |
| 19mb | BB_2GG_r | 33 | 60.0 | GAAGACGCGGTAGTCTAAATCGCTTGACACCCG |
| 19mc | BB_3GG_r | 32 | 60.0 | GAAGACGGGATCAGTCCCCTTCGTTTTTGGGG |
| 19md | f1ori_f | 19 | 61.0 | GCTACTGCGTCACGGATCC |
| 19me | bI_NP_f | 56 | 60.0 | GATTTCTGGAATTCGCGGCCGCTTCTAGAGCAATTGGGACGTATGATTGTTGAGGC |
| 19mf | bI_NP_r | 52 | 60.0 | AGGGCGCGTGGGGATCCGTGACGCAGTAGCAAGAACCTTTTACGGGCACACC |
| 19mg | Lab_pRS3_r | 59 | 60.0 | TGATATCGAATTCCTGCAGCCCGGGGGATCCTAGTATGTTTCGAAGAAGAGATCAGCGC |
| 19mh | Lbu_pVG2_f | 59 | 59.0 | CCCCGCTTGAGCAGACATCACCGTTTAAACTACTAGATGAAAGTGACGAAGGTAGGAGG |
| 19mi | Lbu_pVG2_r | 67 | 59.0 | ACGTTAAGTGGATCCCGGTCGGCATCTACTCTAGTATTAGTTTTCAGACTTTTTCTCTTCCATTTTG |
| 19mj | Lsh_pVG2_f | 54 | 60.0 | CCCCGCTTGAGCAGACATCACCGTTTAAACTACTAGATGGGGAACCTTTTCGGG |
| 19mk | Lsh_pVG2_r | 70 | 60.0 | ACGTTAAGTGGATCCCGGTCGGCATCTACTCTAGTATTACAGGGTATCGTTTGTATTTTCAATCTTAGTC |
| 19ml | Lwa_pVG2_f | 66 | 60.0 | CCCCGCTTGAGCAGACATCACCGTTTAAACTACTAGATGAAAGTCACTAAAGTCGATGGTATTAGC |
| 19mm | Lwa_pVG2_r | 54 | 59.0 | ACGTTAAGTGGATCCCGGTCGGCATCTACTCTAGTATTACTGCTCCGCTGTCGC |
| 19mn | pVG2_f | 23 | 64.0 | AGTAGATGCCGACCGGGATCCAC |
| 19mo | pVG2_r | 29 | 65.0 | GTTTAAACGGTGATGTCTGCTCAAGCGGG |
| 19mp | pVG2_gRNA_r | 16 | 65.0 | AGCGACCGAGGCGGCG |
| 19mq | pVG2_gRNA_f | 32 | 65.0 | CACTGTTCCTTTACGGATATGTGTACTCCGGC |
| 19mr | gRNA_pVG2_r | 58 | 64.0 | CGGAGTACACATATCCGTAAAGGAACAGTGCTGCAGCGGCCGCTACTAGTAAAAAAAC |
| 19ms | S5gRNA_pVG2_f | 62 | 64.0 | GCTCCACGCGGGCGCGCCGCCTCGGTCGCTACTAGCACATACGACCACAGGGTGTGGAAAAC |
| 19mt | Asp_RNA_Kol_f | 15 | 63.0 | CGCGCCGCCTCGGTC |
| 19mu | pVG2_seq_r | 23 | 59.0 | CTCAATCACACCACGGATTCAAG |
| 19mv | pVG2_seq_f | 29 | 59.0 | CAGTATATTCATCTTCCCATCCAAGAACC |
| 19mw | sfGFP_pVG2_f | 55 | 61.0 | CCCCGCTTGAGCAGACATCACCGTTTAAACTACTAGATGCGTAAAGGCGAAGAGC |
| 19mx | sfGFP_pVG2_r | 65 | 63.0 | ACGTTAAGTGGATCCCGGTCGGCATCTACTCTAGTATCATTTGTACAGTTCATCCATACCATGCG |
| 19my | pVG2_mC_r | 27 | 60.0 | CTAGTACATACAACAGAAGGGATTCGC |
| 19mz | pVG2_mC_f | 27 | 59.0 | TACTAGTTTTTTTACTAGTAGCGGCCG |
| 19na | mC_pVG2_r | 58 | 59.0 | GTGCTGCAGCGGCCGCTACTAGTAAAAAAACTAGTATTACTTGTACAGCTCGTCCATG |
| 19nb | mC_pVG2_f | 52 | 60.0 | TGACCACCAGCGAATCCCTTCTGTTGTATGTACTAGATGGTGAGCAAGGGCG |
| 19nc | Bb_Ins_R | 30 | 57.0 | CTAGTATTTCTCCTCTTTCTCTAGTATGTG |
| 19nd | Bb_Ins_F | 27 | 60.0 | TAACGCTGATAGTGCTAGTGTAGATCG |
| 19ne | Npu_C1_F | 73 | 60.0 | CACATACTAGAGAAAGAGGAGAAATACTAGATGATCAAAATAGCCACACGTAAATATTTAGGCAAACAAAATG |
| 19nf | Npu_C1_R | 67 | 60.0 | TATGGTCGCGCTCAACTCCAATGTCATAGACATTTTGTTTGCCTAAATATTTACGTGTGGCTATTTT |
| 19ng | Npu_C2_F | 65 | 64.0 | TCTATGACATTGGAGTTGAGCGCGACCATAATTTTGCACTCAAAAATGGCTTCATAGCTTCTAAC |
| 19nh | Npu_C2_CmB_R | 53 | 62.0 | CTCACCGTCTTTCATGCAGTTAGAAGCTATGAAGCCATTTTTGAGTGCAAAAT |
| 19ni | Cm_B_NpuC_F | 39 | 59.0 | TTCATAGCTTCTAACTGCATGAAAGACGGTGAGCTGGTG |
| 19nj | Cm_Bb_R | 54 | 59.0 | TAGCGATCTACACTAGCACTATCAGCGTTAGATATCAAATTACGCCCCGCCCTG |
| 19nk | Cm_Bb_F | 60 | 59.0 | CACATACTAGAGAAAGAGGAGAAATACTAGATGGAGAAAAAAATCACTGGATATACCACC |
| 19nl | NpuN_B_Cm_F | 48 | 60.0 | CGGAATTTCGTATGGCATGCTTAAGCTATGAAACGGAAATATTGACAG |
| 19nm | Cm_B_NpuN_R | 38 | 59.0 | TTCATAGCTTAAGCATGCCATACGAAATTCCGGATGAG |
| 19nn | NpuN_Bb_R | 58 | 60.0 | TAGCGATCTACACTAGCACTATCAGCGTTATTAATTCGGCAAATTATCAACCCGCATC |
| 19no | Npu_C2_CmA_R | 50 | 62.0 | CTGGTTATAGGTACAGTTAGAAGCTATGAAGCCATTTTTGAGTGCAAAAT |
| 19np | Cm_A_NpuC_F | 40 | 59.0 | TTCATAGCTTCTAACTGTACCTATAACCAGACCGTTCAGC |
| 19nq | Cm_A_NpuN_R | 42 | 59.0 | TTCATAGCTTAAGCACTTTTGAGCAACTGACTGAAATGCCTC |
| 19nr | NpuN_A_Cm_F | 49 | 58.0 | CAGTCAGTTGCTCAAAAGTGCTTAAGCTATGAAACGGAAATATTGACAG |
| 19ns | Hyg_Bb_F | 53 | 60.0 | CACATACTAGAGAAAGAGGAGAAATACTAGATGAAAAAGCCTGAACTCACCGC |
| 19nt | Hyg_NpuN_R | 39 | 64.0 | TCCGTTTCATAGCTTAAGCAAGCCAACCACGGCCTCCAG |
| 19nu | NpuN_Hyg_F | 41 | 59.0 | GTGGTTGGCTTGCTTAAGCTATGAAACGGAAATATTGACAG |
| 19nv | Npu_C2_Hyg_R | 56 | 62.0 | GCGCGTCTGCTGCTCCATACAGTTAGAAGCTATGAAGCCATTTTTGAGTGCAAAAT |
| 19nw | Hyg_NpuC_F | 32 | 61.0 | ATAGCTTCTAACTGTATGGAGCAGCAGACGCG |
| 19nx | Hyg_Bb_R | 53 | 62.0 | TAGCGATCTACACTAGCACTATCAGCGTTACTATTCCTTTGCCCTCGGACGAG |
| 19ny | dPCR_NP_1_f | 20 | 60.0 | CCTGTAGCGGCGCATTAAGC |
| 19nz | dPCR_NP_1_r | 24 | 60.0 | GAGGTGCCGTAAAGCACTAAATCG |
| 19oa | dPCR_NP_2_f | 20 | 60.0 | GTGGTGACCGTGACCCAGGA |
| 19ob | dPCR_NP_2_r | 20 | 60.0 | CGTCCTTCAGCTTCAGCCTC |
| 19oc | dPCR_NP_3_f | 22 | 60.0 | CGGTGCTGCTATCGATGGTTTC |
| 19od | dPCR_NP_3_r | 23 | 60.0 | GACAAAAGGGCGACATTCAACCG |
| 19oe | dPCR_HP_1_f | 22 | 60.0 | CTATGCGCCTGGTCTGTACACC |
| 19of | dPCR_HP_1_r | 21 | 60.0 | CCCCAGCGATTATACCAAGCG |
| 19og | dPCR_HP_2_f | 22 | 60.0 | GCCAGTTCTTTTGGGTATTCCG |
| 19oh | dPCR_HP_2_r | 24 | 60.0 | GCAATAGCTATCTTACCGAAGCCC |
| 19oi | dPCR_HP_3_f | 23 | 60.0 | GATAATTCCGCTCCTTCTGGTGG |
| 19oj | dPCR_HP_3_r | 24 | 60.0 | CGTATTAAATCCTTTGCCCGAACG |
| 19ok | gmtA_892_f | 70 | 70.0 | CTAGGATTTAGACTACCCCAAAAACGAAGGGGACTAAAACGTCACTTCTTCGACCACTTACGATTTAGAC |
| 19ol | gmtA_892_r | 70 | 70.0 | GGTAGTCTAAATCGTAAGTGGTCGAAGAAGTGACGTTTTAGTCCCCTTCGTTTTTGGGGTAGTCTAAATC |
| 19om | rmsA_246_f | 70 | 75.0 | TACCCCAAAAACGAAGGGGACTAAAACCTACCAGCTGGGTGTTGCCCGGATTTAGACTACCCCAAAAACG |
| 19on | RmsA_246_r | 70 | 75.0 | CCTTCGTTTTTGGGGTAGTCTAAATCCGGGCAACACCCAGCTGGTAGGTTTTAGTCCCCTTCGTTTTTGG |
| 19oo | hemF_103_f | 73 | 72.0 | AAGGGGACTAAAACTACTCCTCGGAACAGAAGCCTGATTTAGACTACCCCAAAAACGAAGGGGACTAAAACTA |
| 19op | hemF_103_r | 73 | 71.0 | CTAGTAGTTTTAGTCCCCTTCGTTTTTGGGGTAGTCTAAATCAGGCTTCTGTTCCGAGGAGTAGTTTTAGTCC |
| 19oq | BB_rmsA246_f | 31 | 58.0 | GAAGACGCAAGGTACTAGTAGCGGCCGCTGC |
| 19or | BB_gmtA896_f | 31 | 59.0 | GAAGACGCTACCTACTAGTAGCGGCCGCTGC |
| 19os | BB_HemF103_f | 36 | 60.0 | GAAGACGTTAGTTTTTTTACTAGTAGCGGCCGCTGC |
| 19ot | BB_rmsA246_r | 35 | 58.0 | GAAGACGCGGTAGTCTAAATCGTAAGTGGTCGAAG |
| 19ou | BB_hemF103_r | 36 | 61.0 | GAAGACTGCCTTCGTTTTTGGGGTAGTCTAAATCCG |
| 19ov | BB_S52_r | 27 | 60.0 | GAAGACCTACTAACCTCCCGGCGTGTG |
| 19ow | BB_S51_f | 31 | 61.0 | GAAGACAGAGTATACTAGTAGCGGCCGCTGC |
| 19ox | BB_S52_f | 31 | 56.0 | GAAGACAGCTAGTACTAGTAGCGGCCGCTGC |
| 19oy | Kre5_f | 75 | 71.0 | TACTAGTACTAGGATTTAGACTACCCCAAAAACGAAGGGGACTAAAACATCGCCAACGGGTACTTTAGCGATTTA |
| 19oz | Kre5_r | 75 | 72.0 | AGTCTAAATCGCTAAAGTACCCGTTGGCGATGTTTTAGTCCCCTTCGTTTTTGGGGTAGTCTAAATCCTAGTACT |
| 19pa | GAA1_f | 70 | 72.0 | GACTACCCCAAAAACGAAGGGGACTAAAACATATCCTCAGCCCTCTCGTTGGATTTAGACTACCCCAAAA |
| 19pb | GAA1_r | 70 | 73.0 | TCGTTTTTGGGGTAGTCTAAATCCAACGAGAGGGCTGAGGATATGTTTTAGTCCCCTTCGTTTTTGGGGT |
| 19pc | GLS1_f | 75 | 73.0 | ACGAAGGGGACTAAAACCCAGGATTACGACGATGGTTAGATTTAGACTACCCCAAAAACGAAGGGGACTAAAACT |
| 19pd | GLS1_r | 75 | 72.0 | TAGTAGTTTTAGTCCCCTTCGTTTTTGGGGTAGTCTAAATCTAACCATCGTCGTAATCCTGGGTTTTAGTCCCCT |
| 19pe | BB_Kre5_f | 38 | 62.0 | GAAGACGCGACTGTTTTTTTACTAGTAGCGGCCGCTGC |
| 19pf | BB_GAA1_f | 33 | 56.0 | GAAGACATACGAGTTTTTTTACTAGTAGCGGCC |
| 19pg | BB_GLS1_f | 36 | 61.0 | GAAGACATACTAGTTTTTTTACTAGTAGCGGCCGCT |
| 19ph | BB_Kre5_r | 29 | 60.0 | GAAGACGCAGTACTCTAGAAGCGGCCGCG |
| 19pi | BB_GAA1_r | 31 | 57.0 | GAAGACGCAGTCTAAATCGCTAAAGTACCCG |
| 19pj | BB_GLS1_r | 38 | 61.0 | GAAGACGCTCGTTTTTGGGGTAGTCTAAATCCAACGAG |
| 19pk | RFP_kol_f | 21 | 54.0 | CAAAGCTTACGTTAAACACCC |
| 19pl | BB_3GG_neu_r | 38 | 59.0 | GAAGACGAGTTTTTGGGGTAGTCTAAATCCATTCTGGA |
| 19pm | mCh_Pro_f | 49 | 57.0 | ATGCCGGGCGGACCGGGCGGACCGGGCGGAGGCATGGTGAGCAAGGGCG |
| 19pn | pTXB1_Pro_r | 74 | 56.0 | CATGCCTCCGCCCGGTCCGCCCGGTCCGCCCGGCATATGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTC |
| 19po | pSB3C5_Cas_r | 32 | 59.0 | CTAGTATTTCTCCTCTTTCACTAGTAGCTAGC |
| 19pp | CasX_p35_f | 56 | 60.0 | TAGCTACTAGTGAAAGAGGAGAAATACTAGATGGAGAAGCGTATTAACAAGATTCG |
| 19pq | Lbu_p35_f | 59 | 61.0 | CAGTGCTAGCTACTAGTGAAAGAGGAGAAATACTAGATGAAAGTGACGAAGGTAGGAGG |
| 19pr | Lsh_p35_f | 54 | 60.0 | CAGTGCTAGCTACTAGTGAAAGAGGAGAAATACTAGATGGGGAACCTTTTCGGG |
| 19ps | Lwa_p35_f | 66 | 59.0 | ACAGTGCTAGCTACTAGTGAAAGAGGAGAAATACTAGATGAAAGTCACTAAAGTCGATGGTATTAG |
| 19pt | Col_Pro_f | 19 | 61.0 | TACATATGCCGGGCGGACC |
| 19pu | GG_4_7_f | 33 | 60.0 | GAAGACATCCCAAAAACGAAGGGGACTAAAACC |
| 19pv | GG_4_7_r | 29 | 61.0 | GAAGACATGTGGTGTCATTGCAGCCCTAG |
| 19pw | Lbu_seq_4_r | 26 | 59.0 | CTTCGTCCTGATTACCGGCATTAATC |
| 19px | Lsh_seq_4_r | 37 | 59.0 | CGGTAAATATTTAATATCGTTGTTATTCTCCTCACTG |
| 19py | Lwa_seq_4_r | 22 | 60.0 | CGTGTTCTTCTCCTCCTTGTCC |
| 19pz | TRP_f | 61 | 57.0 | AGCAAACAGGCTCAACATTAAGACTACTAGATGTCTGTTATTAATTTCACAGGTAGTTCTG |
| 19qa | TRP_r | 54 | 57.0 | TCCGTCGGAGGGCTGTCGCTAGTACTATTTCTTAGCATTTTTGACGAAATTTGC |
| 19qb | pSB_TRP_f | 48 | 57.0 | GCAAATTTCGTCAAAAATGCTAAGAAATAGTACTAGCGACAGCCCTCC |
| 19qc | pSB_TRP_r | 58 | 57.0 | AGAACTACCTGTGAAATTAATAACAGACATCTAGTAGTCTTAATGTTGAGCCTGTTTG |
| 19qd | f1ori_seq_f | 18 | 59.0 | CGCTTAATGCGCCGCTAC |
| 19qe | NP_Fus_f | 33 | 60.0 | GAACAAAAGCTTATTTCTGAAGAGGACTTGATG |
| 19qf | NP_Fus_r | 26 | 59.0 | GAGGAAGTTTCCATTACTTTGACCCC |
| 19qg | NP_Fus_Mat_f | 58 | 60.0 | CCAGCCGATGTATGGTGGTGGGTCAGAACAAAAGCTTATTTCTGAAGAGGACTTGATG |
| 19qh | NP_Fus_Mat_r | 51 | 61.0 | GTTTCAGTTGCAGCCAATGCCACATGAGGAAGTTTCCATTACTTTGACCCC |
| 19qi | Opy_NP_f | 48 | 61.0 | GCTGGGGGTCAAAGTAATGGAAACTTCCTCATGGATGGCTGCGTGGTG |
| 19qj | Opy_NP_r | 52 | 61.0 | CATCAAGTCCTCTTCAGAAATAAGCTTTTGTTCTGACCCACCACCTTTCGCG |
| 19qk | Flo_NP_f | 48 | 61.0 | GCTGGGGGTCAAAGTAATGGAAACTTCCTCATGTTTCCGACCGCGCTG |
| 19ql | Flo_NP_r | 53 | 61.0 | CATCAAGTCCTCTTCAGAAATAAGCTTTTGTTCTGACCCACCACCTTTGGTGC |
| 19qm | gRNA13_r | 29 | 62.0 | GTTTTTGGGGTAGTCTAAATCGTGACAGG |
| 19qn | gRNA46_f | 70 | 59.0 | TGTCACGATTTAGACTACCCCAAAAACGAAGGGGACTAAAACGTCACTTCTTCGACCACTTACGATTTAG |
| 19qo | mCh_Pro_f_2 | 75 | 57.0 | TTTAACTTTAAGAAGGAGATATACATATGCCGGGCGGACCGGGCGGACCGGGCGGAGGCATGGTGAGCAAGGGCG |
| 19qp | NP_Fus_Pro_f | 80 | 58.0 | GTAATGGAAACTTCCTCATGCCGGGCGGACCGGGCGGACCGGGCGGAGGCGAACAAAAGCTTATTTCTGAAGAGGACTTG |
| 19qq | NP_Fus_Pro_r | 31 | 60.0 | GGCATGAGGAAGTTTCCATTACTTTGACCCC |
| 19qr | BI_pRS_rev | 65 | 59.0 | TGATATCGAATTCCTGCAGCCCGGGGGATCCAGCCCTAGTAAGACATAAAAAACAAAAAAACTAG |
| 19qs | Mat_for_NP_f | 80 | nan | ATGGAAACTTCCTCATGTGGCATTGGCTGCAACTGAAACCGGGCCAGCCGATGTATGGTGGTGGGTCAGAACAAAAGCTT |
| 19qt | Mat_for_NP_r | 80 | nan | AAGCTTTTGTTCTGACCCACCACCATACATCGGCTGGCCCGGTTTCAGTTGCAGCCAATGCCACATGAGGAAGTTTCCAT |
| 19qu | BB_S52_r_neu | 28 | 28.0 | GAAGACCTTACTAACCTCCCGGCGTGTG |
| 19qv | Cm1_Bb_Ex_F | 63 | 59.0 | GCTTACATAAACAGTAATACAAGGGGTGTTATGATCAAAATAGCCACACGTAAATATTTAGGC |
| 19qw | Cm1_Bb_Ex_R | 46 | 63.0 | CCAGTGTTACAACCAATTAACCAATTCTGATTACGCCCCGCCCTGC |
| 19qx | Cm2_Bb_Ex_F | 60 | 58.0 | TTAAATTGCTAACGCAGTCAGGCACCGTGTATGGAGAAAAAAATCACTGGATATACCACC |
| 19qy | Cm2_Bb_Ex_R | 55 | 59.0 | TTGCCCGTTTTTTTGCCGGAGCTAGTATTATTAATTCGGCAAATTATCAACCCGC |
| 19qz | Hyg1_Bb_Ex_F | 53 | 60.0 | GCTTACATAAACAGTAATACAAGGGGTGTTATGAAAAAGCCTGAACTCACCGC |
| 19ra | Hyg1_Bb_Ex_R | 55 | 59.0 | CCAGTGTTACAACCAATTAACCAATTCTGATTAATTCGGCAAATTATCAACCCGC |
| 19rb | Hyg2_Bb_Ex_F | 63 | 59.0 | TTAAATTGCTAACGCAGTCAGGCACCGTGTATGATCAAAATAGCCACACGTAAATATTTAGGC |
| 19rc | Hyg2_Bb_Ex_R | 53 | 62.0 | TTGCCCGTTTTTTTGCCGGAGCTAGTATTACTATTCCTTTGCCCTCGGACGAG |
| 19rd | Kan_Ins_F | 57 | 55.0 | CACATACTAGAGAAAGAGGAGAAATACTAGTTAGAAAAACTCATCGAGCATCAAATG |
| 19re | Kan_Ins_R | 53 | 61.0 | TAGCGATCTACACTAGCACTATCAGCGTTAATGAGCCATATTCAACGGGAAAC |
| 19rf | Bb_Ex_1X3_R | 30 | 60.0 | TCAGAATTGGTTAATTGGTTGTAACACTGG |
| 19rg | Bb_Ex_1X3_F | 30 | 59.0 | AACACCCCTTGTATTACTGTTTATGTAAGC |
| 19rh | Tet_Ins_F | 57 | 61.0 | CACATACTAGAGAAAGAGGAGAAATACTAGATGAAATCTAACAATGCGCTCATCGTC |
| 19ri | Tet_Ins_R | 49 | 61.0 | TAGCGATCTACACTAGCACTATCAGCGTTATTAGGTCGAGGTGGCCCGG |
| 19rj | Bb_Ex_3X5_R | 18 | 63.0 | ACACGGTGCCTGACTGCG |
| 19rk | Bb_Ex_3X5_F | 25 | 58.0 | TAATACTAGCTCCGGCAAAAAAACG |
| 19rl | Gal1_short_r | 49 | 61.0 | GAATCTTTATTGTTCGGAGCAGTGCGGCGCCTAGTAGCCACCGCGGTGG |
| 19rm | Gal1_short_f | 45 | 61.0 | GAATTGGAGCTCCACCGCGGTGGCTACTAGGCGCCGCACTGCTCC |
| 19rn | Kan_Bb_F | 54 | 60.0 | CACATACTAGAGAAAGAGGAGAAATACTAGATGAGCCATATTCAACGGGAAACG |
| 19ro | Kan_Bb_R | 54 | 57.0 | CGATCTACACTAGCACTATCAGCGTTATTAGAAAAACTCATCGAGCATCAAATG |
| 19rp | Kan_NpuN_R | 43 | 61.0 | TTCATAGCTTAAGCATGCATCATCAGGAGTACGGATAAAATGC |
| 19rq | Kan_NpuC_F | 40 | 59.0 | CTTCATAGCTTCTAACTGTTGGTTACTCACCACTGCGATC |
| 19rr | NpuN_Kan_F | 46 | 61.0 | ACTCCTGATGATGCATGCTTAAGCTATGAAACGGAAATATTGACAG |
| 19rs | NpuC_Kan_R | 45 | 60.0 | AGTGGTGAGTAACCAACAGTTAGAAGCTATGAAGCCATTTTTGAG |
| 19rs | Kan_Bb_Ex_F | 54 | 61.0 | TTAAATTGCTAACGCAGTCAGGCACCGTGTATGAGCCATATTCAACGGGAAACG |
| 19rs | Kan_Bb_Ex_R | 57 | 58.0 | CCAGTGTTACAACCAATTAACCAATTCTGATTAGAAAAACTCATCGAGCATCAAATG |