Team:Bielefeld-CeBiTec/Experiments
Protocols
Protocols
This page
contains a list of various methods applied during our experimental time in the
laboratory. A list of enzymes, polymerases, and other kinds of enzymes is also provided below.
- 50x TAE
- Bradford Assay with ROTI® Nanoquant
- Cas13a Activity Assay
- Cell Lysis French Press
- Cell Lysis Ribolyzer
- Chemically Competent Cells
- CM Medium (Aspergillus)
- Colony PCR with GoTaq (Promega)
- Colony PCR with LongAmp (NEB)
- Colony PCR with Taq Polymerase
- ddPCR
- Endocytosis Test
- Fluorescence Microscopy
- Fungicide Simulation
- Gel and PCR Cleanup Kit (Promega)
- Gel and PCR Cleanup Kit (Qiagen)
- Gibson Assembly
- Golden Gate Assembly
- Heat Shock Transformation
- His-Tag Purification
- IMPACT Protein Purification
- Lab Application Assay
- LB Medium
- Light Stability Assay
- Macherey Nagel Gel and PCR Cleanup Kit
- MALDI-ToF
- Merck Protein Ultrafiltration
- Nanopore Sequencing
- PCR with Q5 Polymerase (NEB)
- PBS Buffer
- pH Sensitivity Assay
- Phage Plaque Assay
- Phage Purification
- Phage Purification: Protein
- Phage Purification: NEB Protocol
- Plasmid Purification Kit (Qiagen)
- Plasmid Purification Kit (Zymo)
- Restriction Ligation Cloning
- SD Medium
- SDS PAGE
- SOC Medium
- Yeast Transformation
- YT Medium
- YPD Medium
Enzymes
Restriction Enzymes
| Enzyme | Manufacturer |
|---|---|
| BamHI (High Fidelity) | NEB |
| BbsI | NEB |
| BbsI (High Fidelity) | NEB |
| DpnI | NEB |
| EcoRI (High Fidelity) | NEB |
| MfeI (High Fidelity) | NEB |
| MunI (FastDigest) | ThermoFischer |
| NcoI (High Fidelity) | NEB |
| NheI (High Fidelity) | NEB |
| NotI (High Fidelity) | NEB |
Polymerase
| Polymerase | Manufacturer |
|---|---|
| GoTaq DNA Polymerase | Promega |
| LongAmp Taq DNA Polymerase | NEB |
| OneTaq | NEB |
| Phusion® High-Fidelity DNA Polymerase | NEB |
| Q5 High-Fidelity DNA Polymerase | NEB |
| Taq DNA Polymerase | NEB |
Others
| Others | Manufacturer |
|---|---|
| Klenow Fragment | Thermo Scientific |
| Klenow Fragment | NEB |
| T4 Ligase | NEB |