Team:ASTWS-China/Safety

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Safety

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Before starting our project, we, ASTWS-China team, received a one-week long training concerning lab safety and equipment operating procedure at Zhejiang University . We learnt about basic safety rules and common microbiology’s techniques in the lab.

After the training, we could utilize the common equipment in lab such as centrifuge properly and proficiently. Moreover, we realized that safety was the most important and we should follow the rules strictly and ask our instructors when encounter problems to avoid dangers to lab technicians or damages in lab. We have included the do not release policy, and deletions as modification rules into our teaching curriculum. As a matter of fact, our project does not include animals or related substances, however, we utilize anti-microbial resistance genes. With the guidelines of iGEM headquarters, we follow our instructors’ rules and the regulation of the lab whenever we were treating with the GMO containing anti-microbial resistance genes. All of our lab work is carried out under the safety regulation of iGEM and provision of Zhejiang University, biology department.

For the human practice part of our experiment, there would always be a guidance counselor following the team to ensure our interview or taking samples from our public fits in our social norm and the University’s moral guideline.

The experimental safety rules are as follows:

1．When entering the lab, you must wear lab coats and exam gloves. If encountering toxic materials, you must wear safety goggles.

2．No personal belongings or food is allowed in the lab.

3．Always keep work stations clean and in good order. After the experiment, it is required for everyone to return their lab equipment and materials according to instruction.

4．Never touch any substance in a non-contamination area with gloves on.

5．All team members should be properly trained for all the procedures before entering the lab. Unauthorized experiments or procedures must not be attempted. Operation of special instruments should be done with the help of a qualified instructor.

6．Safety rules, and the regulations of special equipment are to be strictly followed.

7．Taps should be closed after use to prevent an overflow of water. If an instrument or piece of equipment fails during use, the situation is to be reported to a technician immediately. Gels like culture medium or agarose gel are to be kept away from the sink. Solids are to be cleaned out afterwards, especially from an Eppendorf tube.

8．In case of a fire, all power should be cut off and all flammable materials removed. The situation is to be reported immediately. With no further instruction available, call the police, 110, or 119, immediately.

9．Never operate electrical equipment with wet hands.

10．Flammable materials are to be kept away from open fires.

11．Never taste or intentionally sniff chemicals. Perform manipulations of materials that pose an inhalation hazard in a chemical fume hood to control exposure. Put on safety gears before any manipulation of the toxic substances. Never leave reactions unattended.

12．When handling corrosive materials, gloves and other chemically resistant protective clothing should be worn to protect against skin contact. In case of an accident, immediately clean the exposed areas of skin and seek medical advice.

13．Any experiments involving radioactive materials is to be carried out in special labs and supervised by qualified instructors.

14．In case of a biological spill, wipe up spill and clean the surface with an available, and suitable disinfectant and immediately wash exposed areas of skin with soap and water. Clean the experiment areas afterward, do not allow any out leak of any biological substances.

15．Wear protective eyewear and face shield while operating instruments that produce ultraviolet radiation. Safety glasses should be used when operating UV trans illuminators or gel imaging systems.

16．Waste materials are to be disposed of accordingly.