Team:ASTWS-China/Applied Design

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Applied design

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Due to the final consequences we got from our experiment, we’ve drew the primary pictures regarding the utilization of these three biodegradation systems in real scenarios. We propose that establishing electron circuits is capable of running these genetic parts effectively. These circuits are designed aiming to eliminate antibiotics contaminants in end pipes of sewage working factories. The major design is mainly divided into two separate sections shown below.

R₀ (Constant Resistance): The resistance that possesses a constant value of resistance (R) which is greater than 0 Ω, aiming to keep the circuit from short-outs, preventing potential burning of the model. This R₀ is necessarily installed in any individual circuit in this design.

R₁-Photoresistance: The resistance that possesses a variable R which is determined by the intensity of fluorescence. R will be higher if the fluorescent lights are stronger, while R would remain 0 when no fluorescence is accepted by this R1-Photoresistance.

V (Voltmeter): Monitoring the voltage (V) distributed to the R1-Photoresistance. An additional Part.2’s the promoter is installed on this voltmeter: When V > 0, the promoter will become capable of permitting Part.2’s normal works; when V = 0, the promoter will not work at all.

Part.1 and Part.2: the chambers including oxySp/mec and blaCMY-10, which is preserved by the nanoscaled strainers installed on both ends of chambers, not releasing out to the environment. The wastewater will NOT pass through these chambers if there is no electricity motivation. Both parts have the same resistance of 0 Ω. The Part 2 can work only if both Part.2’s promoter allows and sufficient electricity motivates its working.

Figure 1 Schematic diagram of the circuit design for antibiotics contaminants detection (A) and degradation (B).

Now let’s concentrate on practical utilization of these circuits, assuming these procedures are capitalized on the final stages of digestion tanks or sewage plants several times.

Assuming that detection requires a time of x while the degradation requires a time of y:

The primitive unfiltered wastewater would stay in Cistern 1, undergoing the detecting system and resting on this pool for x. if there are antibiotic contaminants detected, the water will be transferred to Cistern 2, where section 2 participate; if there is not, these liquid would be directly discharged or sent to the next stage of processing. The wastewater in Cistern 2 will not flow until the degrading completion, staying in Cistern 2 for y. Afterward, this wastewater is going to be re-inspected, undergoing section 1, staying in Cistern 2 for another x. This wastewater will stay in Cistern 2 once antibiotics pollutants are still inspected amid them, or they would be directly discharged or sent to the next stage of processing. If wastewater is going to be remained in Cistern 2, initiating another circulation, no more new unfiltered sewerage would income.

The graph below has shown this practical utilization accurately.