Difference between revisions of "Team:Austin UTexas/Notebook"

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   <button class="accordion">Week 8 (July 15 - 21)</button>
 
   <button class="accordion">Week 8 (July 15 - 21)</button>
 
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   <p>Wet Lab: Completed ~11 transformations, ran 4 burden assay plates <br> People: Noor, Jordan, Diya, Ginny, Angela, Shireen, Alex, Michelle, Raul<br><br>
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   <p>Wet Lab: Completed ~15 transformations, ran 4 burden assay plates <br> People: Noor, Jordan, Diya, Ginny, Angela, Shireen, Alex, Michelle, Raul<br><br>
 
Dry Lab: Gathered distribution of DNA parts by type and sub-part number <br>People: Noor, Jordan, Diya, Ginny, Angela, Shireen <br><br>
 
Dry Lab: Gathered distribution of DNA parts by type and sub-part number <br>People: Noor, Jordan, Diya, Ginny, Angela, Shireen <br><br>
 
<img src="https://static.igem.org/mediawiki/2019/d/d6/T--Austin_Utexas--PartDistribution.png" width="40%"> <br><br>
 
<img src="https://static.igem.org/mediawiki/2019/d/d6/T--Austin_Utexas--PartDistribution.png" width="40%"> <br><br>

Revision as of 01:50, 10 October 2019

Notebook

  • mention the names of people in dry lab and wet lab
  • add pictures

    • Wet lab: Collectively completed ~40 transformations, ran 3 burden assay plates (exp007, 008, 009)
    People: Noor, Jordan, Diya, Ginny, Angela, Shireen

    Dry Lab: Python script written to create a record of transformation DNA sequences for later comparison (reference file generation)

    People: Noor, Jordan, Diya, Ginny, Angela, Shireen

    Wet Lab: Completed ~62 transformations total, ran 7 burden assay plates (exp007-013)
    People: Noor, Jordan, Diya, Ginny, Angela, Shireen


    Dry Lab: Wrapping up reference file for all biobrick DNA sequences, wiki graphics are being made, starting interactive simulation of burden
    People: Noor, Jordan, Diya, Ginny, Angela, Shireen

    Wet Lab: Transformations, burden assays
    People: Noor, Jordan, Diya, Ginny, Angela, Shireen

    Dry Lab: Looked at data for burden analysis
    People: Noor, Jordan, Diya, Ginny, Angela, Shireen

    Wet Lab: Completed ~77 transformations total, ran 4 burden assay plates
    People: Noor, Jordan, Diya, Ginny, Angela, Shireen, Alex, Raul

    Dry Lab: Previous burden assay experiments were analyzed in RStudio
    People: Noor, Jordan, Diya, Ginny, Angela, Shireen

    Wet Lab: Transformations, burden assays
    People: Noor, Jordan, Diya, Ginny, Angela, Shireen, Alex, Michelle, Raul

    Dry Lab: Burden analysis
    People: Noor, Jordan, Diya, Ginny, Angela, Shireen

    Wet Lab: Decided to update transformation plan and have a goal of 1000 transformations total, ~100 from commonly used parts and ~900 from the 2018 kit plate.
    People: Noor, Jordan, Diya, Ginny, Angela, Shireen, Alex, Michelle, Raul

    Dry Lab: Planned University of Texas at Arlington, Texas Tech, and Rice University meet up and voice chat, tested out a few parts in breseq (sequence analysis program) with reference file, refined burden scripts for analyzing data
    People: Noor, Jordan, Diya, Ginny, Angela, Shireen

    Wet Lab: Completed ~80 transformations, ran 2 burden assay plates
    People: Noor, Jordan, Diya, Ginny, Angela, Shireen, Alex, Michelle, Raul

    Dry Lab: Created a script to programmatically run breseq
    People: Noor, Jordan, Diya, Ginny, Angela, Shireen

    bla. bla.

    Wet Lab: Completed ~15 transformations, ran 4 burden assay plates
    People: Noor, Jordan, Diya, Ginny, Angela, Shireen, Alex, Michelle, Raul

    Dry Lab: Gathered distribution of DNA parts by type and sub-part number
    People: Noor, Jordan, Diya, Ginny, Angela, Shireen



    Wet Lab: Made new batch of competent cells for transformations because of contamination of old cells, transformations, ran 2 burden assays
    People: Noor, Jordan, Diya, Ginny, Angela, Shireen, Alex, Michelle, Raul

    Dry Lab: Worked on sequence analysis, simulation script, and burden analysis
    People: Noor, Jordan, Diya, Ginny, Angela, Shireen

    Wet Lab: Completed ~30 transformations
    People: Noor, Jordan, Diya, Ginny, Angela, Shireen, Mina, Raul

    Dry Lab: Fixing burden analysis --> refined quantitative interpretation of burden, figured out how to calculate percentage of each type of burden
    People: Noor, Jordan, Diya, Ginny, Angela, Shireen, Mina

    Wet Lab: 8 burden assays done
    People: Noor, Jordan, Diya, Ginny, Angela, Shireen, Mina, Alex, Raul

    Dry Lab: Wiki, burden analysis
    People: Noor, Jordan, Diya, Ginny, Angela, Shireen, Mina

    Wet Lab: Burden assays
    People: Noor, Jordan, Diya, Ginny, Angela, Shireen, Mina, Alex, Raul

    Dry Lab: Made graphs, worked on statistical correction of data, wiki
    People: Noor, Jordan, Diya, Ginny, Angela, Shireen, Mina, Alex

    Wet Lab: Redid some burden assays
    People: Noor, Jordan, Diya, Ginny, Angela, Shireen, Mina, Alex, Raul

    Dry Lab: Collaboration write up, got data from Texas Tech University about calibration strains, wiki
    People: Noor, Jordan, Diya, Ginny, Angela, Shireen, Mina

    Wet Lab: Burden assay of interesting parts
    People: Noor, Jordan, Diya, Ginny, Angela, Shireen, Mina, Raul

    Dry Lab: Wiki
    People: Noor, Jordan, Diya, Ginny, Angela, Shireen, Alex, Mina

    Wet Lab: Burden assays
    People: Noor, Jordan, Diya, Ginny, Angela, Shireen, Mina, Raul

    Dry Lab: Wiki
    People: Noor, Jordan, Diya, Ginny, Angela, Shireen, Michelle, Alex, Raul, Mina

    Wet Lab: Redid some burden assays
    People: Noor, Jordan, Diya, Ginny, Angela, Shireen, Mina, Raul

    Dry Lab: Statistics, wiki
    People: Noor, Jordan, Diya, Ginny, Angela, Shireen, Michelle, Alex, Raul, Mina





    Notebook

    Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.


    What should this page have?

    • Chronological notes of what your team is doing.
    • Brief descriptions of daily important events.
    • Pictures of your progress.
    • Mention who participated in what task.

    Inspiration

    You can see what others teams have done to organize their notes:

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