Team:CSMU Taiwan/Demonstrate

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Overview

In addition to comply to all rules and policies approved by the iGEM Safety Committee (please see our Safety page), we self-evaluate the safety of our project with the Risk Mitigation Plan referring the selective agent and dual use research management regulations of TCDC.

Risk mitigation plan

This year, we design an aptamer-type rapid diagnostic kit called influsensor, trying to solve the disadvantages of screening on the influenza nowadays. (please see our Description page)

There are two main products in our project:

  1. The aptamer with high affinity and specificity designed for rapid diagnostic test
  2. Influensor (aptamer-type rapid diagnostic kit) that is extremely easy for doctors to use

Overall, our the aptamers selected out by the target proteins NPA and HA3 have been proved to possess the properties of high affinity and specificity through our experiments, indicateing that there is a potential to replacing the current antibody-type rapid influenza diagnostic strip. (please see our Entrepreneurship page)

Aptamers

This year, we acquire four successful experimental results related to produce aptamers with high affinity and specificity in our project:

Target protein with optimized sequence

The production of the target protein is the preprocess of SELEX. However, our original yield of NPA protein and NPB protein is too low to supply the need for SELEX. We optimized the sequence and compare the absorbance standard curve before and after the sequence optimization.

The fugures indicate that the protein yield of both NPA protein and NPB protein significantly increased after the sequence optimization.

SELEX

In order to acquire the aptamers of target proteins, we conducted the SELEX experiment. After 6 rounds of SELEX for every protein respectively, the aptamers of each target proteins were obtained. The PDF files below are the sequenced results of the aptamers:

NPA  PDF NPB  PDF HA1  PDF HA3  PDF

ELISA

Rapid diagnostic test strip

(Please see our Results page)

Influsensor

<嵌入 strip, casing, swab照片, 收合>

Influsensor is an aptamer-type rapid diagnostic kit with the characteristics of low cost, fast production and development, high stability, low false-negative rate, and animal free. (Please see our Design page)

Influsensor is made of aptamer-type rapid diagnostic strip, casing, and swab. We design every component of the kit with ingenuity. We also demonstrate the user manual and the video of user guide. All of our designs are aim for making doctors manipulate the kit easily. (Please see our Design page)

User manual

User guide

Because of all the advantages and features of the aptamer, we integrate all the benefits of atpamers and design a model containing all the aspects of a disease: Rapid screening, Prediction, Prevention, and Treatment. The model is called CSMU_Taiwan Influsystem.

CSMU_Taiwan Influsystem

Rapid screening- Aptamer-type rapid diagnostic strip (Influsensor)

Prediction- AI system

Prevention- Air purifier

Treatment- Anti-hemagglutinin drug

(Please see our Description page and Design page)