Team:CSMU Taiwan/Notebook

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Protein

2019  March

March 7th ~ 28th   NPA & NPB Transformation  ,  Small and Large Scale Production

2019  April

April 1st ~ 9th   NPA & NPB Small and Large Scale Production
April 23rd ~ 27th   NPA & NPB Production
April 25th ~ May 3rd   NPA & NPB Production

2019  May

May 1st ~ 12th   NPA & NPB Production
May 8th ~ 12th   NPA & NPB Production

2019  June

June 28th   NPA new & NPB new Transformation
June 29th ~ July 4th   NPA new & NPB new Small Scale Production

2019  July

July 1st ~ 7th   7/2_NPA & NPB Production
July 6th ~ 7th   NPA new & NPB new Small Scale Production
July 14th ~ 15th   NPA new & NPB new Small Scale Production test
July 15th ~ 17th   7/16_NPA & NPB Production
July 22nd ~ 28th   7/23_NPA & NPB  ,  7/24_NPA  ,  7/26_NPA Production
July 29th ~ August 4th   7/30_NPB  ,  7/31_NPA-1 & NPA-2  ,  8/1_NPB-1 & NPB-2

2019  August

August 5th ~ 11st   8/5_NPB-1 & NPB-2  ,  8/9_NPA-1 & NPA-2 Production
August 11th ~ 14th   THU protein & 8/12_NPB Production
August 13th ~ 15th   8/14_NPA & NPB Production
August 15th ~ 17th   TEV protease sequence in pSB1C3 transformation   &   plasmid miniprep of pET29a
August 15th ~ 20th   HA improved Transformation & HA improved Small Scale Production
August 15th ~ 23rd   8/16_NPA & NPB Production
August 16th ~ 17th   8/2_NPB  ,  8/9_NPA-1 & NPA-2 Protein Dialysis
August 17th ~ 18th   8/12_NPB  ,  8/8_NPB-1 & NPA-2 Protein Dialysis
August 17th ~ 21st   TEV PCR  ,  Purification  ,  Digestion
August 20th ~ 27th   8/21_NPA & NPB Production
August 21st ~ September 2nd   8/22_HA improved Production
August 26th ~ 27th   7/24_NPA Protein Purification
August 26th ~ 30th   8/29_NPA Production
August 26th ~ September 6th   TEV sequence plasmid miniprep  ,  PCR  ,  Purification and Digestion

2019  September

September 1st ~ 3rd   New & Old NP Production Comparison
September 5th ~ 7th   MIND collaboration
September 8th ~ 17th   Plasmid miniprep of pET29a plasmid  ,  Ligation  ,  Colony PCR
September 11th ~ 17th   9/12_NPB-1 & NPB-2 Production
September 16th ~ 23rd   Plasmid mini prep  ,  Digestion and Transformation of TEV-pET29a
September 18th ~ 20th   9/19_NPB Production
September 22nd ~ 25th   9/23_NPA & NPB Production
September 26th ~ 27th   TEV Small Scale Production

2019  October

October 2nd ~ 6th   10/3_TEV Production
October 3rd ~ 18th  HA improved & HA original Transformation  ,  Production Comparison
October 15th ~ 18th   10/16_TEV Production

Selex

2019  May
   Quantify protein concentration and Test SELEX condition using SELEX tube.

2019  June

SELEX   NPA   first time

2019  July

PCR condition test

2019  August

SELEX   NPA   second time
SELEX   NPB
SELEX   HA3

2019  September

SELEX   HA1