Team:CSMU Taiwan/Collaborations
iGEM 2019 Taiwan conference
On August 5th , most Taiwan IGEM team got together in Taichung ,thanks to NCKU IGEM team. We presented and showed poster to each other. We challenged each others’ projects and learned a lot from each others. We realized the pros and cons about our project, so we optimized our project after the conference. Last but not least, we became friends.
Presentation in iGEM 2019 Taiwan conference
CCU iGEM team
CCU iGEM team had problems while performing PCR. We shared our protocol with them and tell them the problems that they may suffer. They also wanted to separate their double strand DNA, but they didn’t have any tag in their sequence. We told them that they could add biotin to one strand of double strand DNA ,so that they could separate their double strand DNA. Besides, We wanted to manufacture our rapid diagnostic test, but we didn’t have relevant equipments and techniques. We asked CCU iGEM team for help. We shared our idea and showed them a detailed drawing of the device. They analyzed the design, and gave us advices based on manufacturing difficulties and structural integrity. They drew the picture in Solidworks, stimulated in Ultimaker Cura 4.0. They produced 11 sets of the device made of PLA using 3D printers and delivered to us. Then, we could put our strip inside to use and demonstrate our product!
Online meeting with CCU iGEM team
Meep up with CCU iGEM team
The rapid diagnostic test device made by CCU iGEM team
We were joyful and thankful while receiving the device.
Mingdao iGEM team
This year, team Mingdao aimed to produce carbonic anhydrase (CA) which enhanced CO2 dissolving rate, as well as CYP2E1 to break down chloroform and benzene. We helped Mingdao with purifying carbonic anhydrase from E.coli and protein quantitation. We enabled them to estimate the yield of their proteins in a single production, and predict the efficacy of their products. What’s more, we also invited Mingdao iGEM team to our lab and teached them how to perform over-expression, purification, dialysis and ELISA with our protocol. We are glad to have a chance to mentor a high school team. At mid-July, we had problems doing TA cloning. We asked team Mingdao for help,since they were the experts of TA cloning and gene cloning. They successfully inserted our aptamers into plasmid, and reminded us the tips. After that, we successfully performed TA cloning .
Communicating with teacher and members of team Mingdao at their lab.
We invited Mingdao iGEM team to our lab and teached them how to perform over-expression, purification, dialysis and ELISA
The result of TA cloning helped by Mingdao
The result of over-expression in SDS page that we helped Mingdao
The result of purification in SDS page that we helped Mingdao
The result of purification in Western blot that we helped Mingdao
Tunghai_TAPG
This year, Tunghai_TAPG designed an effective peptide JJ01 from MAP0403 which has been found with effective antimicrobial. They wanted to dissolve E.coli and purify their protein, but they didn’t know how to do. We used ultrasonic reactor to dissolve E.coli and helped them make sure their peptide’s molecular mass.Then, we told them the condition we used, such as adding PMSF preventing peptide from degradation and gave them our protocol, so that they could purify by themselves. Besides, they helped us modify the swab tool of our rapid diagnosis test kits,giving us some suggestions about the way we store our reagent, so that we could make our project more perfect.
Meet up with Tunghai_TAPG iGEM team
The result of over-expression in SDS page that we helped Tunghai_TAPG
original draft of the swab tube
modified version of the draft of the swab tube
NCHU iGEM team
We had several meet up with NCHU iGEM team, we shared ideas with each other. We suggested NCHU iGEM team to add tags on the sequences so that they could purify their proteins and estimated the efficiency of each step of their whole system. Besides, NCHU iGEM suggested that we could use mass spectrometer to perform Qualitative and quantitative analysis.
Online meeting with NCHU iGEM team
Meet up with NCHU iGEM team
iGEM Aptamers Hub
Collaborating Teams: iGEM Athens, iGEM Madrid, iGEM Pasteur-Paris, iGEM Rotterdam, iGEM Strasbourg, iGEM BOKU-Vienna, iGEM Cornell, iGEM CSMU Taiwan, iGEM DUT China A
While conducting our project, we wished many times for there to be a more formal platform for people working with aptamers to share ideas, advice, and feedback. For this reason, most of the teams working with aptamers this year created the iGEM Aptamers Hub. The iGEM Aptamers Hub aimed to enable the use of aptamers during the iGEM competition and encourage their use in laboratories worldwide. We created an ‘‘aptamer registry’’, created a network with aptamer researchers, and provided the community with useful resources to facilitate their research. See our website We offered our own way about selecting aptamers, analyzed its pros and cons, communicated with other teams, and shared ideas with the public. We held group discussions through Skype and WhatsApp in order to decide on important issues concerning the Hub’s organization and function. We shared our own methods about selecting aptamers with others, and received many useful advices from the online meeting. For example, we got the information about RNA-puzzles, so that we could stimulate the aptamers’ 3D structure.
