Team:CSMU Taiwan/Collaborations
iGEM 2019 Taiwan conference
On August 5th , most Taiwan IGEM team got together in Taichung ,thanks to NCKU IGEM team. We presented and showed poster to each other. We challenged each others’ project and learned a lot from others. We realized the pros and cons about our project, so we optimize our project after the conference. Last but not least, we became friends
Presentation in iGEM 2019 Taiwan conference
CCU iGEM team
We want to manufacture our fast screen fast screening test, but we don’t have relevant equipments and techniques. We ask CCU IGEM team for help. We shared our idea and showed them a detailed drawing of the device. They analyzed the design and gave us some advices based on manufacturing difficulties and structural integrity. After confirming all the details, They produced 11 sets of the device made of PLA using 3D printers and delivered to us, so that we can put our strip inside to use and demonstrate our product! Besides, we shared some ideas as well as suggestions about characterization to them. Since we perform SELEX, We have lots of experiments about doing PCR . We gave some advices about their sequences, such as adding biotin to one strand of double strand DNA ,so that they can separate their double strand DNA.
Online meeting with CCU iGEM team
Meep up with CCU iGEM team
The rapid detection device made by CCU iGEM team
We are joyful and thankful while receiving the device.
Mingdao iGEM team
This year, team Mingdao aims to produce carbonic anhydrase (CA) which enhances CO2 dissolving rate, as well as CYP2E1 to break down chloroform and benzene. We helped Mingdao with purifying carbonic anhydrase from E.coli and protein quantitation for them；therefore enabling them to estimate the yield of their proteins in a single production, and predict the efficacy of their products. What’s more, we also invite Mingdao iGEM team to our lab and teach them how to perform over-expression, purification, dialysis and ELISA by our protocol. At mid-July, We had problems when doing TA cloning.We asked team Mingdao for help,since they are the experts of TA cloning and gene cloning. They successfully inserted our aptamers into plasmid, and reminded us the tips while doing experiment. After that, we successfully performed TA cloning .
We went to team Mingdao's lab.
We invite Mingdao iGEM team to our lab and teach them how to perform over-expression, purification, dialysis and ELISA
The result of TA cloning helped by Mingdao
The result of over-expression in SDS page we help Mingdao
The result of purification in SDS page we help Mingdao
The result of purification in Western blot we help Mingdao
Tunghai_TAPG
It’s Tunghai_TAPG’s first time participating in iGEM .We have a great meet up with each other. We gave some advices for them, and they also gave us some awesome ideas. After the meep up, we have a tight connection between each other. We use ultrasonic reactor to dissolve E.coli and help them make sure their polypeptide’s molecular mass.Then, we told them some tips and gave them our protocol, so that they can purify by themselves.
Meet up with Tunghai_TAPG iGEM team
The result of over-expression in SDS page we help Tunghai_TAPG
NCHU iGEM team
We have several meet up with NCHU iGEM team, we share ideas with each other. We suggest NCHU iGEM team to add tag on the sequence so that they can purify their proteins and estimate the efficiency of each step of their whole system. Besides, NCHU iGEM suggests that we can use mass spectrometer to perform Qualitative and quantitative analysis.
Online meeting with NCHU iGEM team
Meet up with NCHU iGEM team
iGEM Aptamers Hub
Collaborating Teams: iGEM Athens, iGEM Madrid, iGEM Pasteur-Paris, iGEM Rotterdam, iGEM Strasbourg, iGEM BOKU-Vienna, iGEM Cornell, iGEM CSMU Taiwan, iGEM DUT China A
While doing our research for our project, we many times wished for there to be a more formal platform for anybody working with aptamers to be able to share ideas, advice, and feedback. For this reason, most of the teams working with aptamers this year created the iGEM Aptamers Hub. The iGEM Aptamers Hub aims to enable the use of aptamers during the iGEM competition and encourage their use in laboratories worldwide. We create an ‘’aptamer registry’’, create a network with aptamer researchers, and provide the community with useful resources to facilitate their research. We offer our own way about selecting aptamers and analyzing its pros and cons, communicating with other team, and share ideas with publics.
