Team:CSMU Taiwan/Improve

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  • Improve
• HP
  • HP Structure
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• Model
• Safety

Sequence Optimization

TEV Cutting Site

Further Applications

Improved Solubility

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Sequence selection and Codon Optimization

The previous part (BBa_K1955000) was documented to be acquired from NCBI with no further indication. The sequence of HA strain(A/WSN 1933/TS61(H1N1)) is based on NCBI(CY010788.1), being 7 bp difference to the precious part(Fig.1).

To express the target protein, we chose to use BL21 E.coli strain with a pET29a vector. In order to improve the expression efficiency of our chassis, we first codon optimized the sequence and checked for illegal cutting sites using ATGme for E.coli.