Part Improvement
During the development of our iGEM project, particularly when designing our Part Collection, we decided to offer Printeria users a wide range of reporter proteins that they could use in their experiments. However it called our attention that some reporter proteins, such as the Yellow Fluorescent Protein (YFP), did not present much information about their use and experimentation, compared to the well-characterized Green fluorescent protein (GFP) or (superfolder Green Fluorescent Protein (sfGFP).
For this reason, the Printeria team has carried out several experiments using YFP as a reporter protein in order to better characterize it. Throughout our project, we have obtained its excitation and emission spectra, as well as the molecules of equivalent fluorescence (MEFL) per cell factor that allows us to convert FOD (Fluorescence/OD ratio) into MEFL.
Once the experiment was carried out, from the results in Figure 1 we can observe that the bacteria growth of both constructions is very similar, and the fluorescence (protein amount) had a clear variation.
Figure 1. Comparison between the transcriptional unit with BBa_K2656021 (YFP) and the one with BBa_K2656020 (YFP LVA tag), and between the experimental and simulation data.
These data were optimized using our model, and the optimized parameters are shown in Table 1. With these parameters it is possible to determine that the protein degradation of the protein with the LVA degradation tag is around twice as much as without the tag. This result shows that the LVA degradation tag improvement can be used for genetic circuits that need a reporter protein with fast degradation.
Optimized parameters |
Values |
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Translation rate p |
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PoI degradation rate dp |
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Dilution rate μ |
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