Difference between revisions of "Team:SMMU-China/Parts"

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             characteristics. Second, we chose some synthetic transcription factors to activate expression of target
 
             characteristics. Second, we chose some synthetic transcription factors to activate expression of target
 
             genes. Moreover, there were soluble molecules to connect the two devices.</p>
 
             genes. Moreover, there were soluble molecules to connect the two devices.</p>
 +
        <div align="center">
 +
            <table class="smmu-table">
 +
                <thead>
 +
                <tr>
 +
                    <th>No.</th>
 +
                    <th>Biobrick Name</th>
 +
                    <th>Description</th>
 +
                </tr>
 +
                </thead>
 +
                <tbody>
 +
                <tr>
 +
                    <td data-label="No.">BBa_K3132000<br>
 +
                        BBa_K3132001<br>
 +
                        BBa_K3132002<br>
 +
                        BBa_K3132003
 +
                    </td>
 +
                    <td data-label="Biobrick Name">Gal4-VP64<br>
 +
                        ZF43-8-VP64<br>
 +
                        ZF21-16-VP64<br>
 +
                        PIP-VP64
 +
                    </td>
 +
                    <td data-label="Description">Synthetic transcription factors fused to intracellular domain of synNotch.</td>
 +
                </tr>
 +
                <tr>
 +
                    <td data-label="No.">BBa_K3132004</td>
 +
                    <td data-label="Biobrick Name">MinimalCMV</td>
 +
                    <td data-label="Description">Fused to promoters to enhance priming activity.</td>
 +
                </tr>
 +
                <tr>
 +
                    <td data-label="No.">BBa_K3132005<br>
 +
                        BBa_K3132006
 +
                    </td>
 +
                    <td data-label="Biobrick Name">anti-HER2 scFV<br>
 +
                        NL domains through cytoplasmic
 +
                    </td>
 +
                    <td data-label="Description">The extracellular and transmembrane domains of synNotch.</td>
 +
                </tr>
 +
                <tr>
 +
                    <td data-label="No.">BBa_K3132020</td>
 +
                    <td data-label="Biobrick Name">SynNotch-anti-HER2 </td>
 +
                    <td data-label="Description">Characterization of synNotch</td>
 +
                </tr>
 +
                <tr>
 +
                    <td data-label="No.">BBa_K3132007<br>
 +
                        BBa_K3132008<br>
 +
                        BBa_K3132009
 +
                    </td>
 +
                    <td data-label="Biobrick Name">anti-GC4N scFV<br>
 +
                        anti-IL15 scFV<br>
 +
                        anti-Flag scFV
 +
                    </td>
 +
                    <td data-label="Description">the extracellular domain of GEMS</td>
 +
                </tr>
 +
                <tr>
 +
                    <td data-label="No.">BBa_K3132016</td>
 +
                    <td data-label="Biobrick Name">Generalized extracellular molecule sensor platform (GEMS)</td>
 +
                    <td data-label="Description">Scaffold and characterization of GEMS</td>
 +
                </tr>
 +
                <tr>
 +
                    <td data-label="No.">BBa_K3132012<br>
 +
                        BBa_K3132013
 +
                    </td>
 +
                    <td data-label="Biobrick Name">PIP-Elk1 transcription factor<br>
 +
                        PIP-Elk1 promoter
 +
                    </td>
 +
                    <td data-label="Description">Transcription factor (SynTF) - synthetic promoter (SynPro) pair for MAPK pathway activated by GEMS</td>
 +
                </tr>
 +
                <tr>
 +
                    <td data-label="No.">BBa_K3132018<br>
 +
                        BBa_K3132019
 +
                    </td>
 +
                    <td data-label="Biobrick Name">8×suntag<br>
 +
                        8×Flag
 +
                    </td>
 +
                    <td data-label="Description">Tag proteins used as soluble signal molecules</td>
 +
                </tr>
 +
                <tr>
 +
                    <td data-label="No.">BBa_K3132021<br>
 +
                        BBa_K3132022
 +
                    </td>
 +
                    <td data-label="Biobrick Name">Cetuximab scFv-CAR<br>
 +
                        trastuzumab4D5-5 scFv-CAR
 +
                    </td>
 +
                    <td data-label="Description">Two structure domains of CAR</td>
 +
                </tr>
 +
                </tbody>
 +
            </table>
 +
        </div>
 +
 
     </div>
 
     </div>
  
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         <p>To enhance the activity of promoters, we fused a MinimalCMV to each promoters, and constructed four types of
 
         <p>To enhance the activity of promoters, we fused a MinimalCMV to each promoters, and constructed four types of
 
             component parts.</p>
 
             component parts.</p>
 +
        <div align="center">
 +
            <table class="smmu-table">
 +
                <thead>
 +
                <tr>
 +
                    <th>No.</th>
 +
                    <th>Biobrick Name</th>
 +
                    <th>Description</th>
 +
                </tr>
 +
                </thead>
 +
                <tbody>
 +
                <tr>
 +
                    <td data-label="No.">BBa_K3132100<br>
 +
                        BBa_K3132101
 +
                    </td>
 +
                    <td data-label="Biobrick Name">UAS_MinimalCMV Promotor<br>
 +
                        PIP_MinimalCMV Promotor
 +
                    </td>
 +
                    <th data-label="Description">Activate expression of CARs and soluble </th>
 +
                </tr>
 +
                <tr>
 +
                    <td data-label="No.">BBa_K3132102<br>
 +
                        BBa_K3132103
 +
                    </td>
 +
                    <td data-label="Biobrick Name">ZF21-16_MinimalCMV Promotor<br>
 +
                        ZF43-8_MinimalCMV Promotor
 +
                    </td>
 +
                    <th data-label="Description">Molecules in the core device</th>
 +
                </tr>
 +
                </tbody>
 +
            </table>
 +
        </div>
 
     </div>
 
     </div>
  
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             of them and got a bifunctional transcription factor that is able to both inhibit and activate the expression
 
             of them and got a bifunctional transcription factor that is able to both inhibit and activate the expression
 
             of target genes.</p>
 
             of target genes.</p>
 +
        <div align="center">
 +
            <table class="smmu-table">
 +
                <thead>
 +
                <tr>
 +
                    <th>No.</th>
 +
                    <th>Biobrick Name</th>
 +
                    <th>Description</th>
 +
                </tr>
 +
                </thead>
 +
                <tbody>
 +
                <tr>
 +
                    <td data-label="No.">BBa_K3132105</td>
 +
                    <td data-label="Biobrick Name">PIP-KRAB-Gal4-VP64</td>
 +
                    <th data-label="Description">Bifunctional transcription factor</th>
 +
                </tr>
 +
                </tbody>
 +
            </table>
 +
        </div>
 
     </div>
 
     </div>
  
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         <p>We collected four types of synthetic receptors and four pairs of synTF-synPro. More information on
 
         <p>We collected four types of synthetic receptors and four pairs of synTF-synPro. More information on
 
             <a href="https://2019.igem.org/Team:SMMU-China/Part_Collection"
 
             <a href="https://2019.igem.org/Team:SMMU-China/Part_Collection"
−
               style="text-decoration: underline; color: #3b52b6; font-weight: bold">Parts collection.</a></p>
+
               style="text-decoration: underline; color: #3b52b6; font-weight: bold">Parts collection.</a></p><br><br>
 
     </div>
 
     </div>
  

Revision as of 13:33, 21 October 2019

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Parts Overview
This is a descriptive paragraph.

In 2019, SMMU-China has submitted 29 parts. We divide our parts into 3 groups, basic parts, component parts and an improvement part. We also established two parts collections respectively collected synthetic receptors and synthetic transcription factor (SynTF) - synthetic promoter (SynPro) pairs we have constructed.

Basic Parts

We have constructed a cellular cascade device consists of many basic parts. First, we set several receptors. In the core device, we used synNotch and CAR as our synthetic receptors, while GEMS and MESA was used in the peripherals. All of the receptors consist of several structural domains, with each of them has their own characteristics. Second, we chose some synthetic transcription factors to activate expression of target genes. Moreover, there were soluble molecules to connect the two devices.

No. Biobrick Name Description
BBa_K3132000
BBa_K3132001
BBa_K3132002
BBa_K3132003 		Gal4-VP64
ZF43-8-VP64
ZF21-16-VP64
PIP-VP64 		Synthetic transcription factors fused to intracellular domain of synNotch.
BBa_K3132004 MinimalCMV Fused to promoters to enhance priming activity.
BBa_K3132005
BBa_K3132006 		anti-HER2 scFV
NL domains through cytoplasmic 		The extracellular and transmembrane domains of synNotch.
BBa_K3132020 SynNotch-anti-HER2 Characterization of synNotch
BBa_K3132007
BBa_K3132008
BBa_K3132009 		anti-GC4N scFV
anti-IL15 scFV
anti-Flag scFV 		the extracellular domain of GEMS
BBa_K3132016 Generalized extracellular molecule sensor platform (GEMS) Scaffold and characterization of GEMS
BBa_K3132012
BBa_K3132013 		PIP-Elk1 transcription factor
PIP-Elk1 promoter 		Transcription factor (SynTF) - synthetic promoter (SynPro) pair for MAPK pathway activated by GEMS
BBa_K3132018
BBa_K3132019 		8×suntag
8×Flag 		Tag proteins used as soluble signal molecules
BBa_K3132021
BBa_K3132022 		Cetuximab scFv-CAR
trastuzumab4D5-5 scFv-CAR 		Two structure domains of CAR

Component parts

To enhance the activity of promoters, we fused a MinimalCMV to each promoters, and constructed four types of component parts.

No. Biobrick Name Description
BBa_K3132100
BBa_K3132101 		UAS_MinimalCMV Promotor
PIP_MinimalCMV Promotor 		Activate expression of CARs and soluble
BBa_K3132102
BBa_K3132103 		ZF21-16_MinimalCMV Promotor
ZF43-8_MinimalCMV Promotor 		Molecules in the core device

Improvement parts

Based on the transcription factor PIP-KRAB (BBa_K2446045) which has inhibitory function that have been submitted by Fudan 2017 and a new TF Gal4-VP64 (BBa_K3132000) which has activation function, we fused both of them and got a bifunctional transcription factor that is able to both inhibit and activate the expression of target genes.

No. Biobrick Name Description
BBa_K3132105 PIP-KRAB-Gal4-VP64 Bifunctional transcription factor

Parts collection

We collected four types of synthetic receptors and four pairs of synTF-synPro. More information on Parts collection.



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E-Mail: igem-smmu@smmu.edu.cn
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