Difference between revisions of "Team:SMMU-China/Parts"
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| − | <link rel="stylesheet" href="https://2019.igem.org/wiki/index.php?title=Template:SMMU-China/CSS_main&action=raw&ctype=text/css" /> | + | <link rel="stylesheet" |
| + | href="https://2019.igem.org/wiki/index.php?title=Template:SMMU-China/CSS_main&action=raw&ctype=text/css"/> | ||
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<style type="text/css"> | <style type="text/css"> | ||
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</style> | </style> | ||
| − | + | <div class="big_framework"> | |
| − | + | <div class="index-nav"> | |
| − | + | <div class="index-nav-frame clearfix"> | |
| − | + | <div class="nav-line"> | |
| − | + | <div class="index-nav-frame-logo-mobile"> | |
| − | + | <img src="https://static.igem.org/mediawiki/2019/f/f5/T--SMMU-China--wukong_logo.png" alt="Wukong"> | |
| − | + | </div> | |
| − | + | </div> | |
| − | + | <div class="nav-small" tabindex="-1"> | |
| − | + | <div class="nav-small-focus" tabindex="-1"> | |
| − | + | ||
| − | + | </div> | |
| − | + | <img src="https://static.igem.org/mediawiki/2019/7/70/T--SMMU-China--header_icon.png"/> | |
| − | + | </div> | |
| − | + | <div class="index-nav-frame-logo"> | |
| − | + | <img src="https://static.igem.org/mediawiki/2019/f/f5/T--SMMU-China--wukong_logo.png" alt="Wukong"> | |
| − | + | </div> | |
| − | + | <div class="index-nav-frame-line first-one" tabindex="-1"> | |
| − | + | <a href="https://2019.igem.org/Team:SMMU-China">Home</a> | |
| − | + | <div class="index-nav-frame-line-center need-to-be-hidden"> | |
| − | + | <div class="index-nav-frame-line-li"> | |
| − | + | <a href="https://2019.igem.org/Team:SMMU-China">Home</a> | |
| − | + | </div> | |
| − | + | </div> | |
| − | + | <div class="index-nav-frame-line-focus" tabindex="-1"></div> | |
| − | + | </div> | |
| − | + | <div class="index-nav-frame-line" tabindex="-1"> | |
| − | + | PROJECT | |
| − | + | <div class="index-nav-frame-line-center"> | |
| − | + | <div class="index-nav-frame-line-li"> | |
| − | + | <a href="https://2019.igem.org/Team:SMMU-China/Description">Description</a> | |
| − | + | </div> | |
| − | + | <div class="index-nav-frame-line-li"> | |
| − | + | <a href="https://2019.igem.org/Team:SMMU-China/Design">Design</a> | |
| − | + | </div> | |
| − | + | <div class="index-nav-frame-line-li"> | |
| − | + | <a href="https://2019.igem.org/Team:SMMU-China/Model">Model</a> | |
| − | + | </div> | |
| − | + | <div class="index-nav-frame-line-li"> | |
| − | + | <a href="https://2019.igem.org/Team:SMMU-China/Software">Software</a> | |
| − | + | </div> | |
| − | + | <div class="index-nav-frame-line-li"> | |
| − | + | <a href="https://2019.igem.org/Team:SMMU-China/Notebook">Notebook</a> | |
| − | + | </div> | |
| − | + | <div class="index-nav-frame-line-li"> | |
| − | + | <a href="https://2019.igem.org/Team:SMMU-China/Demonstrate">Demonstrate</a> | |
| − | + | </div> | |
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| − | + | </div> | |
| − | + | <div class="index-nav-frame-line-focus" tabindex="-1"></div> | |
| − | + | </div> | |
| − | + | <div class="index-nav-frame-line active" tabindex="-1"> | |
| − | + | PARTS | |
| − | + | <div class="index-nav-frame-line-center"> | |
| − | + | <div class="index-nav-frame-line-li"> | |
| − | + | <a href="https://2019.igem.org/Team:SMMU-China/Parts">Parts Overview</a> | |
| − | + | </div> | |
| − | + | <div class="index-nav-frame-line-li"> | |
| − | + | <a href="https://2019.igem.org/Team:SMMU-China/Basic_Part">Basic Parts</a> | |
| − | + | </div> | |
| − | + | <div class="index-nav-frame-line-li"> | |
| − | + | <a href="https://2019.igem.org/Team:SMMU-China/Composite_Part">Composite Parts</a> | |
| − | + | </div> | |
| − | + | <div class="index-nav-frame-line-li"> | |
| − | + | <a href="https://2019.igem.org/Team:SMMU-China/Part_Collection">Part Collection</a> | |
| − | + | </div> | |
| − | + | </div> | |
| − | + | <div class="index-nav-frame-line-focus" tabindex="-1"></div> | |
| − | + | </div> | |
| − | + | <div class="index-nav-frame-line" tabindex="-1"> | |
| − | + | <a href="https://2019.igem.org/Team:SMMU-China/Safety">Safety</a> | |
| − | + | <div class="index-nav-frame-line-center need-to-be-hidden"> | |
| − | + | <div class="index-nav-frame-line-li"> | |
| − | + | <a href="https://2019.igem.org/Team:SMMU-China/Safety">Safety</a> | |
| − | + | </div> | |
| − | + | </div> | |
| − | + | <div class="index-nav-frame-line-focus" tabindex="-1"></div> | |
| − | + | </div> | |
| − | + | <div class="index-nav-frame-line HP-button" tabindex="-1"> | |
| − | + | HUMAN PRACTICES | |
| − | + | <div class="index-nav-frame-line-center"> | |
| − | + | <div class="index-nav-frame-line-li"> | |
| − | + | <a href="https://2019.igem.org/Team:SMMU-China/Human_Practices">Human Practices</a> | |
| − | + | </div> | |
| − | + | <div class="index-nav-frame-line-li"> | |
| − | + | <a href="https://2019.igem.org/Team:SMMU-China/Public_Engagement">Education & Engagement</a> | |
| − | + | </div> | |
| − | + | </div> | |
| − | + | <div class="index-nav-frame-line-focus" tabindex="-1"></div> | |
| − | + | </div> | |
| − | + | <div class="index-nav-frame-line" tabindex="-1"> | |
| − | + | Team | |
| − | + | <div class="index-nav-frame-line-center"> | |
| − | + | <div class="index-nav-frame-line-li"> | |
| − | + | <a href="https://2019.igem.org/Team:SMMU-China/Team">Team</a> | |
| − | + | </div> | |
| − | + | <div class="index-nav-frame-line-li"> | |
| − | + | <a href="https://2019.igem.org/Team:SMMU-China/Attributions">Attributions</a> | |
| − | + | </div> | |
| − | + | </div> | |
| − | + | <div class="index-nav-frame-line-focus" tabindex="-1"></div> | |
| − | + | </div> | |
| − | + | </div> | |
| − | + | </div> | |
| − | + | ||
| − | + | <div class="blank_framework"></div> | |
| + | |||
| + | <div class="page_banner"> | ||
| + | <img src="https://static.igem.org/mediawiki/2019/8/82/T--SMMU-China--page_banner_default.jpg" | ||
| + | alt="page_banner_default"> | ||
| + | </div> | ||
| + | |||
| + | <div class="page_big_title_box"> | ||
| + | <div class="page_big_title">Parts Overview</div> | ||
| + | <div class="page_big_subtitle">This is a descriptive paragraph.</div> | ||
| + | </div> | ||
| − | + | <div class="medium_framework"> | |
| − | + | <p>In 2019, SMMU-China has submitted 29 parts. We divide our parts into 3 groups, basic parts, component parts | |
| − | + | and an improvement part. We also established two parts collections respectively collected synthetic | |
| + | receptors and synthetic transcription factor (SynTF) - synthetic promoter (SynPro) pairs we have | ||
| + | constructed.</p> | ||
| + | </div> | ||
| − | + | <div class="medium_framework"> | |
| − | + | <h1>Basic Parts</h1> | |
| − | + | <p>We have constructed a cellular cascade device consists of many basic parts. First, we set several receptors. | |
| − | + | In the core device, we used synNotch and CAR as our synthetic receptors, while GEMS and MESA was used in the | |
| + | peripherals. All of the receptors consist of several structural domains, with each of them has their own | ||
| + | characteristics. Second, we chose some synthetic transcription factors to activate expression of target | ||
| + | genes. Moreover, there were soluble molecules to connect the two devices.</p> | ||
| + | </div> | ||
| − | + | <div class="medium_framework"> | |
| − | + | <h1>Component parts</h1> | |
| − | + | <p>To enhance the activity of promoters, we fused a MinimalCMV to each promoters, and constructed four types of | |
| − | + | component parts.</p> | |
| + | </div> | ||
| − | + | <div class="medium_framework"> | |
| − | + | <h1>Improvement parts</h1> | |
| − | + | <p>Based on the transcription factor PIP-KRAB (BBa_K2446045) which has inhibitory function that have been | |
| − | + | submitted by Fudan 2017 and a new TF Gal4-VP64 (BBa_K3132000) which has activation function, we fused both | |
| + | of them and got a bifunctional transcription factor that is able to both inhibit and activate the expression | ||
| + | of target genes.</p> | ||
| + | </div> | ||
| − | + | <div class="medium_framework"> | |
| − | + | <h1>Parts collection</h1> | |
| − | + | <p>We collected four types of synthetic receptors and four pairs of synTF-synPro. More information on | |
| − | + | <a href="https://2019.igem.org/Team:SMMU-China/Part_Collection" | |
| + | style="text-decoration: underline; color: #3b52b6; font-weight: bold">Parts collection.</a></p> | ||
| + | </div> | ||
| − | + | <div class="footer"> | |
| − | + | <div class="footer_left"> | |
| − | + | <div class="footer_title">Contact Us</div> | |
| − | + | <div class="footer_text"> | |
| − | + | E-Mail: igem-smmu@smmu.edu.cn<br> | |
| − | + | Phone: +86 021-81870925 | |
| − | + | </div> | |
| − | + | </div> | |
| − | + | <div class="footer_right"> | |
| − | + | <div class="footer_title"></div> | |
| − | + | <div class="footer_text"> | |
| − | + | © 2019. SMMU. All rights reserved. | |
| − | + | </div> | |
| − | + | </div> | |
| − | + | </div> | |
| − | + | </div> | |
</html> | </html> | ||
Revision as of 13:11, 21 October 2019
Parts Overview
This is a descriptive paragraph.
In 2019, SMMU-China has submitted 29 parts. We divide our parts into 3 groups, basic parts, component parts and an improvement part. We also established two parts collections respectively collected synthetic receptors and synthetic transcription factor (SynTF) - synthetic promoter (SynPro) pairs we have constructed.
Basic Parts
We have constructed a cellular cascade device consists of many basic parts. First, we set several receptors. In the core device, we used synNotch and CAR as our synthetic receptors, while GEMS and MESA was used in the peripherals. All of the receptors consist of several structural domains, with each of them has their own characteristics. Second, we chose some synthetic transcription factors to activate expression of target genes. Moreover, there were soluble molecules to connect the two devices.
Component parts
To enhance the activity of promoters, we fused a MinimalCMV to each promoters, and constructed four types of component parts.
Improvement parts
Based on the transcription factor PIP-KRAB (BBa_K2446045) which has inhibitory function that have been submitted by Fudan 2017 and a new TF Gal4-VP64 (BBa_K3132000) which has activation function, we fused both of them and got a bifunctional transcription factor that is able to both inhibit and activate the expression of target genes.
Parts collection
We collected four types of synthetic receptors and four pairs of synTF-synPro. More information on Parts collection.