Difference between revisions of "Team:SMMU-China/Basic Part"
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interest and give it a new mission in our nanobots <a href="https://2019.igem.org/Team:SMMU-China" | interest and give it a new mission in our nanobots <a href="https://2019.igem.org/Team:SMMU-China" | ||
style="text-decoration: underline">WuKong</a>.</p> | style="text-decoration: underline">WuKong</a>.</p> | ||
| + | <img style="width: 40%;" src="https://static.igem.org/mediawiki/2019/5/53/T--SMMU-China--parts_basicparts_1.png" | ||
| + | alt="smmu_demonstrate_1" style="margin: 10px 0px;"> | ||
| + | <center>Figure 1. the structure and mechanism of MAPK-GEMS receptor. a.Three domains that mainly constructed | ||
| + | GEMS. b.Mechanism of MAPK-GEMS. | ||
| + | </center> | ||
</div> | </div> | ||
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<p>GEMS is a dimer of two subunits, each was constructed by three domains, an extracellular domain as an | <p>GEMS is a dimer of two subunits, each was constructed by three domains, an extracellular domain as an | ||
anti-suntag scFv, a scaffold transmembrane domain derived from EpoR (erythropoietin receptor) and a | anti-suntag scFv, a scaffold transmembrane domain derived from EpoR (erythropoietin receptor) and a | ||
| − | FGFR1(fibroblast growth factor receptor 1) intracellular domain that induces MAPK pathway <a style="text-decoration: underline">(Figure 1a)</a>. When | + | FGFR1(fibroblast growth factor receptor 1) intracellular domain that induces MAPK pathway <a |
| + | style="text-decoration: underline">(Figure 1a)</a>. When | ||
the MAPK-GEMS recognizes the ligand, each receptor subunit will rotate around its own axis for about 100°, | the MAPK-GEMS recognizes the ligand, each receptor subunit will rotate around its own axis for about 100°, | ||
leads to an activation of transcription factor PIP-Elk which then modulate expression of reporter | leads to an activation of transcription factor PIP-Elk which then modulate expression of reporter | ||
Revision as of 10:40, 21 October 2019
Our favorite basic part - GEMS
This part is a highly generalizable synthetic receptor that has a wild sensing of input signals and effective activation of gene expression. As developed in 2015, this receptor still contains much possibility for transformation in synthetic biology. Our team determined to use this receptor, make some transformation as interest and give it a new mission in our nanobots WuKong.
Structure and mechanism of GEMS
GEMS is a dimer of two subunits, each was constructed by three domains, an extracellular domain as an anti-suntag scFv, a scaffold transmembrane domain derived from EpoR (erythropoietin receptor) and a FGFR1(fibroblast growth factor receptor 1) intracellular domain that induces MAPK pathway (Figure 1a). When the MAPK-GEMS recognizes the ligand, each receptor subunit will rotate around its own axis for about 100°, leads to an activation of transcription factor PIP-Elk which then modulate expression of reporter gene (Figure 1b).
DEMO PART 3
Your team has been approved and you are ready to start the iGEM season! Your team has been approved and you are ready to start the iGEM season! Your team has been approved and you are ready to start the iGEM season! Your team has been approved and you are ready to start the iGEM season! Your team has been approved and you are ready to start the iGEM season! Your team has been approved and you are ready to start the iGEM season! Your team has been approved and you are ready to start the iGEM season!