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<h3 class="h3Attr">CCU iGEM team</h3> | <h3 class="h3Attr">CCU iGEM team</h3> | ||
− | <p> We wanted to manufacture our | + | <p> We wanted to manufacture our rapid diagnostic test, but we didn’t have relevant equipments and techniques. We asked CCU IGEM team for help. We shared our idea and showed them a detailed drawing of the device. They analyzed the design and gave us some advices based on manufacturing difficulties and structural integrity. After confirming all the details, They produced 11 sets of the device made of PLA using 3D printers and delivered to us, so that we can put our strip inside to use and demonstrate our product! |
Besides, we shared some ideas as well as suggestions about characterization to them. Since we performed SELEX, we had lots of experiments about doing PCR . We gave some advices about their sequences, such as adding biotin to one strand of double strand DNA ,so that they could separate their double strand DNA. | Besides, we shared some ideas as well as suggestions about characterization to them. Since we performed SELEX, we had lots of experiments about doing PCR . We gave some advices about their sequences, such as adding biotin to one strand of double strand DNA ,so that they could separate their double strand DNA. | ||
Revision as of 06:27, 20 October 2019
![](https://static.igem.org/mediawiki/2019/9/94/T--CSMU_Taiwan--collaboration_.png)
iGEM 2019 Taiwan conference
On August 5th , most Taiwan IGEM team got together in Taichung ,thanks to NCKU IGEM team. We presented and showed poster to each other. We challenged each others’ project and learned a lot from each others. We realized the pros and cons about our project, so we optimize our project after the conference. Last but not least, we became friends.
![“figure 1](https://static.igem.org/mediawiki/2019/2/2a/T--CSMU_Taiwan--taiwanconference.jpeg)
CCU iGEM team
We wanted to manufacture our rapid diagnostic test, but we didn’t have relevant equipments and techniques. We asked CCU IGEM team for help. We shared our idea and showed them a detailed drawing of the device. They analyzed the design and gave us some advices based on manufacturing difficulties and structural integrity. After confirming all the details, They produced 11 sets of the device made of PLA using 3D printers and delivered to us, so that we can put our strip inside to use and demonstrate our product! Besides, we shared some ideas as well as suggestions about characterization to them. Since we performed SELEX, we had lots of experiments about doing PCR . We gave some advices about their sequences, such as adding biotin to one strand of double strand DNA ,so that they could separate their double strand DNA.
![“figure 1](https://static.igem.org/mediawiki/2019/3/3e/T--CSMU_Taiwan--ccu1.jpeg)
![](https://static.igem.org/mediawiki/2019/6/67/T--CSMU_Taiwan--ccu4.jpeg)
![](https://static.igem.org/mediawiki/2019/e/e4/T--CSMU_Taiwan--ccu2.jpeg)
![](https://static.igem.org/mediawiki/2019/0/0c/T--CSMU_Taiwan--ccu3.jpeg)
Mingdao iGEM team
This year, team Mingdao aimed to produce carbonic anhydrase (CA) which enhanced CO2 dissolving rate, as well as CYP2E1 to break down chloroform and benzene. We helped Mingdao with purifying carbonic anhydrase from E.coli and protein quantitation for them;therefore enabling them to estimate the yield of their proteins in a single production, and predict the efficacy of their products. What’s more, we also invited Mingdao iGEM team to our lab and teached them how to perform over-expression, purification, dialysis and ELISA by our protocol. At mid-July, we had problems doing TA cloning.We asked team Mingdao for help,since they are the experts of TA cloning and gene cloning. They successfully inserted our aptamers into plasmid, and reminded us the tips while doing experiment. After that, we successfully performed TA cloning .
![“figure 1](https://static.igem.org/mediawiki/2019/7/7a/T--CSMU_Taiwan--mind1.jpeg)
![“figure 1](https://static.igem.org/mediawiki/2019/2/24/T--CSMU_Taiwan--mind2.jpeg)
![“figure 1](https://static.igem.org/mediawiki/2019/3/3e/T--CSMU_Taiwan--mind6.jpeg)
![“figure 1](https://static.igem.org/mediawiki/2019/9/90/T--CSMU_Taiwan--mind3.jpeg)
![“figure 1](https://static.igem.org/mediawiki/2019/8/83/T--CSMU_Taiwan--mind4.jpeg)
![“figure 1](https://static.igem.org/mediawiki/2019/1/16/T--CSMU_Taiwan--mind5.jpeg)
Tunghai_TAPG
It’s Tunghai_TAPG’s first time participating in iGEM .We had a great meet up with each other. We gave some advices for them, and they also gave us some awesome ideas. After the meep up, we had a tight connection between each other. We used ultrasonic reactor to dissolve E.coli and helped them make sure their polypeptide’s molecular mass.Then, we told them some tips and gave them our protocol, so that they could purify by themselves.
![](https://static.igem.org/mediawiki/2019/7/79/T--CSMU_Taiwan--tung3.jpeg)
![“figure 1](https://static.igem.org/mediawiki/2019/3/30/T--CSMU_Taiwan--tung1.jpeg)
NCHU iGEM team
We had several meet up with NCHU iGEM team, we shared ideas with each other. We suggested NCHU iGEM team to add tag on the sequence so that they could purify their proteins and estimated the efficiency of each step of their whole system. Besides, NCHU iGEM suggested that we could use mass spectrometer to perform Qualitative and quantitative analysis.
![“figure 1](https://static.igem.org/mediawiki/2019/f/f2/T--CSMU_Taiwan--nchu.jpeg)
![“figure 1](https://static.igem.org/mediawiki/2019/8/89/T--CSMU_Taiwan--nchu1.jpeg)
iGEM Aptamers Hub
Collaborating Teams: iGEM Athens, iGEM Madrid, iGEM Pasteur-Paris, iGEM Rotterdam, iGEM Strasbourg, iGEM BOKU-Vienna, iGEM Cornell, iGEM CSMU Taiwan, iGEM DUT China A
While doing our research for our project, we wished many times for there to be a more formal platform for anybody working with aptamers to be able to share ideas, advice, and feedback. For this reason, most of the teams working with aptamers this year created the iGEM Aptamers Hub. The iGEM Aptamers Hub aimed to enable the use of aptamers during the iGEM competition and encourage their use in laboratories worldwide. We created an ‘‘aptamer registry’’, created a network with aptamer researchers, and provided the community with useful resources to facilitate their research. We offered our own way about selecting aptamers, analyzed its pros and cons, communicated with other team, and shared ideas with publics.We held group discussions through Skype and WhatsApp in order to decide on important issues concerning the Hub’s organization and function. We share our own methods about selecting aptamers to others, and get a lot from the online meeting. For example, we get the information about RNA-puzzles, so that we can stimulate the aptamers’ 3D structure.
![](https://static.igem.org/mediawiki/2019/8/8a/T--CSMU_Taiwan--aptamerhub.jpeg)