Difference between revisions of "Team:CSMU Taiwan/Improve"

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                   <h3>header</h3>
 
                   <h3>header</h3>
 
<h3>Sequence selection and Codon Optimization</h4>
 
<h3>Sequence selection and Codon Optimization</h4>
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<h4>The previous part (BBa_K1955000) was documented to be acquired from NCBI with no further indication. The sequence of HA strain(A/WSN 1933/TS61(H1N1)) is based on <a href=” https://www.ncbi.nlm.nih.gov/nucleotide/CY010788.1?report=genbank&log$=nuclalign&blast_rank=2&RID=F439K4B5015">NCBI(CY010788.1)</a>, being 7 bp difference to the precious part(Fig.1). </h4>
+
<p>The previous part (BBa_K1955000) was documented to be acquired from NCBI with no further indication. The sequence of HA strain(A/WSN 1933/TS61(H1N1)) is based on <a href=” https://www.ncbi.nlm.nih.gov/nucleotide/CY010788.1?report=genbank&log$=nuclalign&blast_rank=2&RID=F439K4B5015" target="blank">NCBI(CY010788.1)</a>, being 7 bp difference to the precious part(Fig.1). </p>
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<h4>To express the target protein, we chose to use BL21 E.coli strain with a pET29a vector. In order to improve the expression efficiency of our chassis, we first codon optimized the sequence and checked for illegal cutting sites using ATGme for E.coli. </h4>
+
<p>To express the target protein, we chose to use BL21 E.coli strain with a pET29a vector. In order to improve the expression efficiency of our chassis, we first codon optimized the sequence and checked for illegal cutting sites using ATGme for E.coli. </p>
  
  

Revision as of 14:29, 16 October 2019

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Sequence Optimization

TEV Cutting Site

Further Applications

Improved Solubility

header

Sequence selection and Codon Optimization

The previous part (BBa_K1955000) was documented to be acquired from NCBI with no further indication. The sequence of HA strain(A/WSN 1933/TS61(H1N1)) is based on NCBI(CY010788.1), being 7 bp difference to the precious part(Fig.1).

To express the target protein, we chose to use BL21 E.coli strain with a pET29a vector. In order to improve the expression efficiency of our chassis, we first codon optimized the sequence and checked for illegal cutting sites using ATGme for E.coli.