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+ | <li><a href="https://2019.igem.org/Team:XMU-China/Human_Practices"><span>Overview</span></a></li> | ||
+ | <li><a href="https://2019.igem.org/Team:XMU-China/Human_Practices#Silver"><span>Silver</span></a></li> | ||
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+ | <li><a href="#"><span>PROJECT</span></a> | ||
+ | <ul> | ||
+ | <li><a href="https://2019.igem.org/Team:XMU-China/Description"><span>Description</span></a></li> | ||
+ | <li><a href="https://2019.igem.org/Team:XMU-China/Design"><span>Design</span></a></li> | ||
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</div> | </div> | ||
+ | <div class="title"> | ||
+ | <img class="top_image" src="https://static.igem.org/mediawiki/2019/3/32/T--XMU-China--parts.png"> | ||
+ | </div> | ||
+ | <nav class="Quick-navigation"> | ||
+ | <div class="Quick-navigation_word"> | ||
+ | <img src="https://static.igem.org/mediawiki/2019/9/9e/T--XMU-China--rignt0.png"> | ||
+ | <a href="#Basic_Parts" class="Quick-navigation-item"> | ||
+ | <img id="turn_img" src="https://static.igem.org/mediawiki/2019/d/db/T--XMU-China--right1.png"> | ||
+ | <a href="#Basic_Parts"id="Quick_A">Basic Parts</a></a> | ||
+ | <a href="#Composite_Parts" class="Quick-navigation-item" > | ||
+ | <img id="turn_img" src="https://static.igem.org/mediawiki/2019/c/cd/T--XMU-China--right2.png"> | ||
+ | <a href="#Composite_Parts" id="Quick_B">Composite Parts</a></a> | ||
+ | <a href="#Part_Collection" class="Quick-navigation-item"> | ||
+ | <img id="turn_img" src="https://static.igem.org/mediawiki/2019/1/1c/T--XMU-China--right3.png"> | ||
+ | <a href="#Part_Collection" id="Quick_C">Part Collection</a></a> | ||
+ | </div> | ||
+ | </nav> | ||
+ | |||
+ | <div class="main"> | ||
+ | <section class="js-scroll-step"> | ||
+ | <div class="headline"><br></div> | ||
+ | <p class="indent">This year, among the 41 parts designed, ten are basic parts and the other are composite ones. All of the parts are designed by ourselves, and most of them have been tested to insure their reliability. You can <span><a class="click_here" href="https://2019.igem.org/Team:XMU-China/Design"> click here </a></span> to learn more about our project to see their specific function, or click on the part numbers when browse website (<span><a class="click_here" href="http://parts.igem.org/Main_Page"> parts.igem.org </a></span>).</p> | ||
+ | </section> | ||
+ | <section id="Basic_Parts" class="js-scroll-step"> | ||
+ | <div class="headline"> | ||
+ | <a class="share" href="https://2019.igem.org/Team:XMU-China/Basic_Part"> Basic Parts</a> | ||
+ | </div> | ||
+ | <p class="indent">Among the ten basic parts designed this year by ourselves, <span><a class="click_here" href="http://parts.igem.org/Part:BBa_K2922040 ">BBa_K2922040 </a></span> is our favorite one, which comes from human genomes. The gene enables engineering bacteria to form a vesicle which will have great applications in the enrichment of substrate (such as metal ions) through extracellular membrane and the enhancement of cell stress resistance. Besides, many other basic parts have also been designed to construct our project. | ||
+ | </p> | ||
+ | <br> | ||
+ | <div class="table_container"> | ||
+ | <table class="table"> | ||
+ | <tr> | ||
+ | <th>Favorite</th> | ||
+ | <th>Register Name</th> | ||
+ | <th>Code used in Lab</th> | ||
+ | <th>Description</th> | ||
+ | <th>Types</th> | ||
+ | <th>Lenth</th> | ||
+ | <th>Designer</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>R</td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922040">BBa_K2922040</a></td> | ||
+ | <td>CAV1</td> | ||
+ | <td>Caveolin-1 coding region</td> | ||
+ | <td>Coding</td> | ||
+ | <td>537</td> | ||
+ | <td>Gezhi Xiao</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922000">BBa_K2922000</a></td> | ||
+ | <td>y-bgl1A</td> | ||
+ | <td>The protein beta-glucosidase<br> with protein YebF fused to its<br> N-terminus by GS Linker.</td> | ||
+ | <td>Coding</td> | ||
+ | <td>1764</td> | ||
+ | <td>Jiyang Zheng</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922001">BBa_K2922001</a></td> | ||
+ | <td>y-cenA</td> | ||
+ | <td>The protein endoglucanase A<br> | ||
+ | with protein YebF fused to its <br> | ||
+ | N-terminus by GS Linker.</td> | ||
+ | <td>Coding</td> | ||
+ | <td>1731</td> | ||
+ | <td>Junhong Chen</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922002">BBa_K2922002</a></td> | ||
+ | <td>y-cex</td> | ||
+ | <td>The protein exoglucanase<br> | ||
+ | with protein YebF fused to its<br> | ||
+ | N-terminus by GS Linker. </td> | ||
+ | <td>Coding</td> | ||
+ | <td>1839</td> | ||
+ | <td>Ruofan Yang</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922024">BBa_K2922024</a></td> | ||
+ | <td>Colicin-E1</td> | ||
+ | <td>Colicin-E1 coding region</td> | ||
+ | <td>Coding</td> | ||
+ | <td>1569</td> | ||
+ | <td>Yi Lin</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922025">BBa_K2922025</a></td> | ||
+ | <td>Eimm</td> | ||
+ | <td>Colicin-E1 immunity protein coding region</td> | ||
+ | <td>Coding</td> | ||
+ | <td>342</td> | ||
+ | <td>Yi Lin</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922026">BBa_K2922026</a></td> | ||
+ | <td>Ekil</td> | ||
+ | <td>Lysis protein for colicin-E1 coding region</td> | ||
+ | <td>Coding</td> | ||
+ | <td>138</td> | ||
+ | <td>Yi Lin</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922027">BBa_K2922027</a></td> | ||
+ | <td>Colicin-N</td> | ||
+ | <td>Colicin-N coding region</td> | ||
+ | <td>Coding</td> | ||
+ | <td>1168</td> | ||
+ | <td>Huilin Guo</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922028">BBa_K2922028</a></td> | ||
+ | <td>Nimm</td> | ||
+ | <td>Colicin-N immunity protein coding region</td> | ||
+ | <td>Regulatory</td> | ||
+ | <td>396</td> | ||
+ | <td>Huilin Guo</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922029">BBa_K2922029</a></td> | ||
+ | <td>Nkil</td> | ||
+ | <td>Lysis protein for colicin-N<br> | ||
+ | coding region</td> | ||
+ | <td>Coding</td> | ||
+ | <td>159</td> | ||
+ | <td>Huilin Guo</td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | </div> | ||
+ | </section> | ||
+ | <section id="Composite_Parts" class="js-scroll-step"> | ||
+ | <div class="headline"> | ||
+ | <a class="share" href="https://2019.igem.org/Team:XMU-China/Composite_Part"> Composite Parts</a> | ||
+ | </div> | ||
+ | <p class="indent">A series of composite parts have been designed this year, and most of them are useful for our project. And our favorite composite part is <span><a class="click_here" href="http://parts.igem.org/Part:BBa_K2922009 ">BBa_K2922009 </a></span>, which contains the sequence of the protein Kil. Kil protein, which accumulates in the periplasmic space of the bacteria, can increase the periplasmic space and improved the membrane permeability of bacteria. Therefore, the protein inside the bacteria can secrete out of bacteria with the help from Kil protein. The use of proper intensity of the promoter can not only achieve the purpose of secretion but also prevent side effects of cell lysis and death. After a series of experiments, J23109 is determined as the most proper promoter.</p> | ||
+ | <br> | ||
+ | <div class="table_container"> | ||
+ | <table class="table"> | ||
+ | <tr><span> | ||
+ | <th>Favorite</th> | ||
+ | <th>Register Name</th> | ||
+ | <th>Code used in Lab</th> | ||
+ | <th>Description</th> | ||
+ | <th>Types</th> | ||
+ | <th>Lenth</th> | ||
+ | <th>Designer</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>R</td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922009">BBa_K2922009</a></td> | ||
+ | <td>109-kil</td> | ||
+ | <td>The expression of Kil protein<br> | ||
+ | by J23109 promoter</td> | ||
+ | <td>Composite</td> | ||
+ | <td>248</td> | ||
+ | <td>Fude Chen</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922006">BBa_K2922006</a></td> | ||
+ | <td>T7-y-bgl1A</td> | ||
+ | <td>The high level secretion <br>of | ||
+ | <a class="click_here" href="http://parts.igem.org/Part:BBa_K2922000">BBa_K2922000</a> <br>by | ||
+ | T7 promoter</td> | ||
+ | <td>Composite</td> | ||
+ | <td>1882</td> | ||
+ | <td>Tianyu Feng</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922007">BBa_K2922007</a></td> | ||
+ | <td>T7-y-cenA</td> | ||
+ | <td>The high level expression <br>of | ||
+ | <a class="click_here" href="http://parts.igem.org/Part:BBa_K2922001">BBa_K2922001</a> <br>by | ||
+ | T7 promoter</td> | ||
+ | <td>Composite</td> | ||
+ | <td>1849</td> | ||
+ | <td>Tianyu Feng</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922008">BBa_K2922008</a></td> | ||
+ | <td>T7-y-cex</td> | ||
+ | <td>The high level expression<br> of | ||
+ | <a class="click_here" href="http://parts.igem.org/Part:BBa_K2922002">BBa_K2922002</a> <br>by | ||
+ | T7 promoter</td> | ||
+ | <td>Composite</td> | ||
+ | <td>1957</td> | ||
+ | <td>Tianyu Feng</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922010">BBa_K2922010</a></td> | ||
+ | <td>112-kil</td> | ||
+ | <td>The expression of Kil protein<br> | ||
+ | by J23112 promoter</td> | ||
+ | <td>Composite</td> | ||
+ | <td>248</td> | ||
+ | <td>Fude Chen</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922011">BBa_K2922011</a></td> | ||
+ | <td>114-kil</td> | ||
+ | <td>The expression of Kil protein<br> | ||
+ | by J23114 promoter</td> | ||
+ | <td>Composite</td> | ||
+ | <td>248</td> | ||
+ | <td>Fude Chen</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922012">BBa_K2922012</a></td> | ||
+ | <td>109-kil-T7-bgl1A</td> | ||
+ | <td>The secretion of <br>beta-glucosidase<br> | ||
+ | by <a href="http://parts.igem.org/Part:BBa_K2922009">BBa_K2922009</a>.</td> | ||
+ | <td>Composite</td> | ||
+ | <td>1760</td> | ||
+ | <td>Jisheng Xie</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922013">BBa_K2922013</a></td> | ||
+ | <td>112-kil-T7-bgl1A</td> | ||
+ | <td>The secretion of <br>beta-glucosidase<br> | ||
+ | by <a href="http://parts.igem.org/Part:BBa_K2922010">BBa_K2922010</a>.</td> | ||
+ | <td>Composite</td> | ||
+ | <td>1760</td> | ||
+ | <td>Jisheng Xie</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922014">BBa_K2922014</a></td> | ||
+ | <td>114-kil-T7-bgl1A</td> | ||
+ | <td>The secretion of <br>beta-glucosidase<br> | ||
+ | by <a href="http://parts.igem.org/Part:BBa_K2922011">BBa_K2922011</a>.</td> | ||
+ | <td>Composite</td> | ||
+ | <td>1760</td> | ||
+ | <td>Jisheng Xie</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922015">BBa_K2922015</a></td> | ||
+ | <td>109-kil-T7-cenA</td> | ||
+ | <td>The secretion of endoglucanase<br> | ||
+ | by <a href="http://parts.igem.org/Part:BBa_K2922009">BBa_K2922009</a>.</td> | ||
+ | <td>Composite</td> | ||
+ | <td>1727</td> | ||
+ | <td>Zinuo Huang</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922016">BBa_K2922016</a></td> | ||
+ | <td>112-kil-T7-cenA</td> | ||
+ | <td>The secretion of endoglucanase<br> | ||
+ | by <a href="http://parts.igem.org/Part:BBa_K2922010">BBa_K2922010</a>.</td> | ||
+ | <td>Composite</td> | ||
+ | <td>1727</td> | ||
+ | <td>Zinuo Huang</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922017">BBa_K2922017</a></td> | ||
+ | <td>114-kil-T7-cenA</td> | ||
+ | <td>The secretion of endoglucanase<br> | ||
+ | by <a href="http://parts.igem.org/Part:BBa_K2922011">BBa_K2922011</a>.</td> | ||
+ | <td>Composite</td> | ||
+ | <td>1727</td> | ||
+ | <td>Zinuo Huang</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922018">BBa_K2922018</a></td> | ||
+ | <td>109-kil-T7-cex</td> | ||
+ | <td>The secretion of exoglucanase<br> | ||
+ | by <a href="http://parts.igem.org/Part:BBa_K2922009">BBa_K2922009</a>.</td> | ||
+ | <td>Composite</td> | ||
+ | <td>1835</td> | ||
+ | <td>Shuling Xiao</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922019">BBa_K2922019</a></td> | ||
+ | <td>112-kil-T7-cex</td> | ||
+ | <td>The secretion of exoglucanase<br> | ||
+ | by <a href="http://parts.igem.org/Part:BBa_K2922010">BBa_K2922010</a>.</td> | ||
+ | <td>Composite</td> | ||
+ | <td>1835</td> | ||
+ | <td>Shuling Xiao</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922020">BBa_K2922020</a></td> | ||
+ | <td>114-kil-T7-cex</td> | ||
+ | <td>The secretion of exoglucanase<br> | ||
+ | by <a href="http://parts.igem.org/Part:BBa_K2922011">BBa_K2922011</a>.</td> | ||
+ | <td>Composite</td> | ||
+ | <td>1835</td> | ||
+ | <td>Shuling Xiao</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922021">BBa_K2922021</a></td> | ||
+ | <td>109-kil-T7-cenA-T7-bgl1A</td> | ||
+ | <td>A composite part linked<br> by <a href="http://parts.igem.org/Part:BBa_K2922015">BBa_K2922015</a> and <br>the high level expression <br>of beta-glucosidase <br>by T7 promoter </td> | ||
+ | <td>Composite</td> | ||
+ | <td>3159</td> | ||
+ | <td>Xv Jiang</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922022">BBa_K2922022</a></td> | ||
+ | <td>109-kil-T7-cex-T7-bgl1A</td> | ||
+ | <td>A composite part linked<br> by <a href="http://parts.igem.org/Part:BBa_K2922018">BBa_K2922018</a> and<br> the high level expression<br> of beta-glucosidase <br>by T7 promoter </td> | ||
+ | <td>Composite</td> | ||
+ | <td>3267</td> | ||
+ | <td>Xv Jiang</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922023">BBa_K2922023</a></td> | ||
+ | <td>109-kil-T7-cex-T7-bgl1A-mRFP</td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2623022">BBa_K2623022</a> with <br>mRFP reporter</td> | ||
+ | <td>Composite</td> | ||
+ | <td>4100</td> | ||
+ | <td>Xv Jiang</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922030">BBa_K2922030</a></td> | ||
+ | <td>TEimm</td> | ||
+ | <td>A composite part linked <br>by <a href="http://parts.igem.org/Part:BBa_K525998">BBa_K525998</a> <br>and <a href="http://parts.igem.org/Part:BBa_K2922025">BBa_K2922025</a></td> | ||
+ | <td>Composite</td> | ||
+ | <td>488</td> | ||
+ | <td>Xingyu Chen</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922031">BBa_K2922031</a></td> | ||
+ | <td>TEkil</td> | ||
+ | <td>A composite part linked <br>by <a href="http://parts.igem.org/Part:BBa_K525998">BBa_K525998</a> <br>and <a href="http://parts.igem.org/Part:BBa_K2922026">BBa_K2922026</a></td> | ||
+ | <td>Composite</td> | ||
+ | <td>284</td> | ||
+ | <td>Xingyu Chen</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922032">BBa_K2922032</a></td> | ||
+ | <td>TNimm</td> | ||
+ | <td>A composite part linked <br>by <a href="http://parts.igem.org/Part:BBa_K525998">BBa_K525998</a> <br>and <a href="http://parts.igem.org/Part:BBa_K2922028">BBa_K2922028</a></td> | ||
+ | <td>Composite</td> | ||
+ | <td>542</td> | ||
+ | <td>Xingyu Chen</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922033">BBa_K2922033</a></td> | ||
+ | <td>TNkil</td> | ||
+ | <td>A composite part linked <br>by <a href="http://parts.igem.org/Part:BBa_K525998">BBa_K525998</a> <br>and <a href="http://parts.igem.org/Part:BBa_K2922029">BBa_K2922029</a></td> | ||
+ | <td>Composite</td> | ||
+ | <td>305</td> | ||
+ | <td>Yunyue Shi</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922034">BBa_K2922034</a></td> | ||
+ | <td>TN</td> | ||
+ | <td>The colicin-N operon under<br> T7 promoter control</td> | ||
+ | <td>Composite</td> | ||
+ | <td>1923</td> | ||
+ | <td>Yunyue Shi</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922035">BBa_K2922035</a></td> | ||
+ | <td>PN</td> | ||
+ | <td>The colicin-N operon under<br> Arabinose promoter control</td> | ||
+ | <td>Composite</td> | ||
+ | <td>2021</td> | ||
+ | <td>Yunyue Shi</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922036">BBa_K2922036</a></td> | ||
+ | <td>TE</td> | ||
+ | <td>The colicin-E1 operon under T7 promoter control</td> | ||
+ | <td>Composite</td> | ||
+ | <td>2247</td> | ||
+ | <td>Man Yang</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922037">BBa_K2922036</a></td> | ||
+ | <td>PE</td> | ||
+ | <td>The colicin-E1 operon under pBAD Arabinose promoter control</td> | ||
+ | <td>Composite</td> | ||
+ | <td>2345</td> | ||
+ | <td>Man Yang</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922038">BBa_K2922036</a></td> | ||
+ | <td>TND</td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2623034">BBa_K2623034</a> with gfasPurple chromoprotein reporter</td> | ||
+ | <td>Composite</td> | ||
+ | <td>2749</td> | ||
+ | <td>Man Yang</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922039">BBa_K2922039</a></td> | ||
+ | <td>PEF</td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2623037">BBa_K2623037</a> with amajLime chromoprotein reporter</td> | ||
+ | <td>Composite</td> | ||
+ | <td>3195</td> | ||
+ | <td>Gezhi Xiao</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922042">BBa_K2922042</a></td> | ||
+ | <td>T7-CAV1</td> | ||
+ | <td>The high level expression<br> of <a href="http://parts.igem.org/Part:BBa_K2922040">BBa_K2922040</a> <br>by T7 promoter</td> | ||
+ | <td>Composite</td> | ||
+ | <td>720</td> | ||
+ | <td>Yuan Li</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922043">BBa_K2922043</a></td> | ||
+ | <td>PbBD</td> | ||
+ | <td>Expression system of<br> DsbA-PbBD coding periplasm<br> display of lead binding peptide<br> with T7 promoter </td> | ||
+ | <td>Composite</td> | ||
+ | <td>987</td> | ||
+ | <td>Yuan Li</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922044">BBa_K2922044</a></td> | ||
+ | <td>MBP-CAV1</td> | ||
+ | <td>Co-expression system of<br> periplasm display of lead <br>binding peptide and caveolin-1<br> for enhancing lead <br>absorption efficiency</td> | ||
+ | <td>Composite</td> | ||
+ | <td>1930</td> | ||
+ | <td>Yuan Li</td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | </div> | ||
+ | </section> | ||
+ | <section id="Part_Collection" class="js-scroll-step"> | ||
+ | <div class="headline"> | ||
+ | <a class="share" href="https://2019.igem.org/Team:XMU-China/Part_Collection"> Part Collection</a> | ||
+ | </div> | ||
+ | <p class="indent">This year, we have formulated a Part Collection related to secretion of intracellular proteins, which includes two major parts: one is secretion achieved with the help of YebF in the form of fusion proteins, the other is the expression of Kil protein by promoters of different intensities, thus changing cell permeability to different degrees and finally realizing secretion. For our Part Collection, the efficiency of three different intracellular proteins we utilized were well tested, recorded in detail and completely uploaded to the registry, which would be instructive in the field of design and characterization for many teams. | ||
+ | </p> | ||
+ | <br> | ||
+ | <div class="table_container"> | ||
+ | <table class="table"> | ||
+ | <tr><span> | ||
+ | <th>Register Name</th> | ||
+ | <th>Code used in Lab</th> | ||
+ | <th>Description</th> | ||
+ | <th>Types</th> | ||
+ | <th>Lenth</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922000">BBa_K2922000</a></td> | ||
+ | <td>y-bgl1A</td> | ||
+ | <td>The protein beta-glucosidase<br> with protein YebF fused to its<br> N-terminus by GS Linker.</td> | ||
+ | <td>Coding</td> | ||
+ | <td>1764</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922001">BBa_K2922001</a></td> | ||
+ | <td>y-cenA</td> | ||
+ | <td>The protein endoglucanase A<br> | ||
+ | with protein YebF fused to its <br> | ||
+ | N-terminus by GS Linker.</td> | ||
+ | <td>Coding</td> | ||
+ | <td>1731</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922002">BBa_K2922002</a></td> | ||
+ | <td>y-cex</td> | ||
+ | <td>The protein exoglucanase<br> | ||
+ | with protein YebF fused to its<br> | ||
+ | N-terminus by GS Linker. </td> | ||
+ | <td>Coding</td> | ||
+ | <td>1839</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922009">BBa_K2922009</a></td> | ||
+ | <td>109-kil</td> | ||
+ | <td>The expression of Kil protein<br> | ||
+ | by J23109 promoter</td> | ||
+ | <td>Composite</td> | ||
+ | <td>248</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922010">BBa_K2922010</a></td> | ||
+ | <td>112-kil</td> | ||
+ | <td>The expression of Kil protein<br> | ||
+ | by J23112 promoter</td> | ||
+ | <td>Composite</td> | ||
+ | <td>248</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922011">BBa_K2922011</a></td> | ||
+ | <td>114-kil</td> | ||
+ | <td>The expression of Kil protein<br> | ||
+ | by J23114 promoter</td> | ||
+ | <td>Composite</td> | ||
+ | <td>248</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922012">BBa_K2922012</a></td> | ||
+ | <td>109-kil-T7-bgl1A</td> | ||
+ | <td>The secretion of <br>beta-glucosidase<br> | ||
+ | by <a href="http://parts.igem.org/Part:BBa_K2922009">BBa_K2922009</a>.</td> | ||
+ | <td>Composite</td> | ||
+ | <td>1760</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922013">BBa_K2922013</a></td> | ||
+ | <td>112-kil-T7-bgl1A</td> | ||
+ | <td>The secretion of <br>beta-glucosidase<br> | ||
+ | by <a href="http://parts.igem.org/Part:BBa_K2922010">BBa_K2922010</a>.</td> | ||
+ | <td>Composite</td> | ||
+ | <td>1760</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922014">BBa_K2922014</a></td> | ||
+ | <td>114-kil-T7-bgl1A</td> | ||
+ | <td>The secretion of <br>beta-glucosidase<br> | ||
+ | by <a href="http://parts.igem.org/Part:BBa_K2922011">BBa_K2922011</a>.</td> | ||
+ | <td>Composite</td> | ||
+ | <td>1760</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922015">BBa_K2922015</a></td> | ||
+ | <td>109-kil-T7-cenA</td> | ||
+ | <td>The secretion of endoglucanase<br> | ||
+ | by <a href="http://parts.igem.org/Part:BBa_K2922009">BBa_K2922009</a>.</td> | ||
+ | <td>Composite</td> | ||
+ | <td>1727</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922016">BBa_K2922016</a></td> | ||
+ | <td>112-kil-T7-cenA</td> | ||
+ | <td>The secretion of endoglucanase<br> | ||
+ | by <a href="http://parts.igem.org/Part:BBa_K2922010">BBa_K2922010</a>.</td> | ||
+ | <td>Composite</td> | ||
+ | <td>1727</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922017">BBa_K2922017</a></td> | ||
+ | <td>114-kil-T7-cenA</td> | ||
+ | <td>The secretion of endoglucanase<br> | ||
+ | by <a href="http://parts.igem.org/Part:BBa_K2922011">BBa_K2922011</a>.</td> | ||
+ | <td>Composite</td> | ||
+ | <td>1727</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922018">BBa_K2922018</a></td> | ||
+ | <td>109-kil-T7-cex</td> | ||
+ | <td>The secretion of exoglucanase<br> | ||
+ | by <a href="http://parts.igem.org/Part:BBa_K2922009">BBa_K2922009</a>.</td> | ||
+ | <td>Composite</td> | ||
+ | <td>1835</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922019">BBa_K2922019</a></td> | ||
+ | <td>112-kil-T7-cex</td> | ||
+ | <td>The secretion of exoglucanase<br> | ||
+ | by <a href="http://parts.igem.org/Part:BBa_K2922010">BBa_K2922010</a>.</td> | ||
+ | <td>Composite</td> | ||
+ | <td>1835</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2922020">BBa_K2922020</a></td> | ||
+ | <td>114-kil-T7-cex</td> | ||
+ | <td>The secretion of exoglucanase<br> | ||
+ | by <a href="http://parts.igem.org/Part:BBa_K2922011">BBa_K2922011</a>.</td> | ||
+ | <td>Composite</td> | ||
+ | <td>1835</td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | </div> | ||
+ | </section> | ||
+ | </div> | ||
+ | <!-- <script type="text/javascript" src="js/right.js"></script> --> | ||
+ | <script src="https://2019.igem.org/Team:XMU-China/js/right?action=raw&ctype=text/javascript"></script> | ||
+ | |||
+ | <div class="footer"> | ||
+ | <div class="footer_bottom"> | ||
+ | <p>Xiamen University, Fujian, China</p> | ||
+ | <p>No.422, Siming South Road, Fujian, P.R.China 361005</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | </body> | ||
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Latest revision as of 03:43, 22 October 2019
![](https://static.igem.org/mediawiki/2019/3/32/T--XMU-China--parts.png)
This year, among the 41 parts designed, ten are basic parts and the other are composite ones. All of the parts are designed by ourselves, and most of them have been tested to insure their reliability. You can click here to learn more about our project to see their specific function, or click on the part numbers when browse website ( parts.igem.org ).
Among the ten basic parts designed this year by ourselves, BBa_K2922040 is our favorite one, which comes from human genomes. The gene enables engineering bacteria to form a vesicle which will have great applications in the enrichment of substrate (such as metal ions) through extracellular membrane and the enhancement of cell stress resistance. Besides, many other basic parts have also been designed to construct our project.
Favorite | Register Name | Code used in Lab | Description | Types | Lenth | Designer |
---|---|---|---|---|---|---|
R | BBa_K2922040 | CAV1 | Caveolin-1 coding region | Coding | 537 | Gezhi Xiao |
BBa_K2922000 | y-bgl1A | The protein beta-glucosidase with protein YebF fused to its N-terminus by GS Linker. |
Coding | 1764 | Jiyang Zheng | |
BBa_K2922001 | y-cenA | The protein endoglucanase A with protein YebF fused to its N-terminus by GS Linker. |
Coding | 1731 | Junhong Chen | |
BBa_K2922002 | y-cex | The protein exoglucanase with protein YebF fused to its N-terminus by GS Linker. |
Coding | 1839 | Ruofan Yang | |
BBa_K2922024 | Colicin-E1 | Colicin-E1 coding region | Coding | 1569 | Yi Lin | |
BBa_K2922025 | Eimm | Colicin-E1 immunity protein coding region | Coding | 342 | Yi Lin | |
BBa_K2922026 | Ekil | Lysis protein for colicin-E1 coding region | Coding | 138 | Yi Lin | |
BBa_K2922027 | Colicin-N | Colicin-N coding region | Coding | 1168 | Huilin Guo | |
BBa_K2922028 | Nimm | Colicin-N immunity protein coding region | Regulatory | 396 | Huilin Guo | |
BBa_K2922029 | Nkil | Lysis protein for colicin-N coding region |
Coding | 159 | Huilin Guo |
A series of composite parts have been designed this year, and most of them are useful for our project. And our favorite composite part is BBa_K2922009 , which contains the sequence of the protein Kil. Kil protein, which accumulates in the periplasmic space of the bacteria, can increase the periplasmic space and improved the membrane permeability of bacteria. Therefore, the protein inside the bacteria can secrete out of bacteria with the help from Kil protein. The use of proper intensity of the promoter can not only achieve the purpose of secretion but also prevent side effects of cell lysis and death. After a series of experiments, J23109 is determined as the most proper promoter.
Favorite | Register Name | Code used in Lab | Description | Types | Lenth | Designer |
---|---|---|---|---|---|---|
R | BBa_K2922009 | 109-kil | The expression of Kil protein by J23109 promoter |
Composite | 248 | Fude Chen |
BBa_K2922006 | T7-y-bgl1A | The high level secretion of BBa_K2922000 by T7 promoter |
Composite | 1882 | Tianyu Feng | |
BBa_K2922007 | T7-y-cenA | The high level expression of BBa_K2922001 by T7 promoter |
Composite | 1849 | Tianyu Feng | |
BBa_K2922008 | T7-y-cex | The high level expression of BBa_K2922002 by T7 promoter |
Composite | 1957 | Tianyu Feng | |
BBa_K2922010 | 112-kil | The expression of Kil protein by J23112 promoter |
Composite | 248 | Fude Chen | |
BBa_K2922011 | 114-kil | The expression of Kil protein by J23114 promoter |
Composite | 248 | Fude Chen | |
BBa_K2922012 | 109-kil-T7-bgl1A | The secretion of beta-glucosidase by BBa_K2922009. |
Composite | 1760 | Jisheng Xie | |
BBa_K2922013 | 112-kil-T7-bgl1A | The secretion of beta-glucosidase by BBa_K2922010. |
Composite | 1760 | Jisheng Xie | |
BBa_K2922014 | 114-kil-T7-bgl1A | The secretion of beta-glucosidase by BBa_K2922011. |
Composite | 1760 | Jisheng Xie | |
BBa_K2922015 | 109-kil-T7-cenA | The secretion of endoglucanase by BBa_K2922009. |
Composite | 1727 | Zinuo Huang | |
BBa_K2922016 | 112-kil-T7-cenA | The secretion of endoglucanase by BBa_K2922010. |
Composite | 1727 | Zinuo Huang | |
BBa_K2922017 | 114-kil-T7-cenA | The secretion of endoglucanase by BBa_K2922011. |
Composite | 1727 | Zinuo Huang | |
BBa_K2922018 | 109-kil-T7-cex | The secretion of exoglucanase by BBa_K2922009. |
Composite | 1835 | Shuling Xiao | |
BBa_K2922019 | 112-kil-T7-cex | The secretion of exoglucanase by BBa_K2922010. |
Composite | 1835 | Shuling Xiao | |
BBa_K2922020 | 114-kil-T7-cex | The secretion of exoglucanase by BBa_K2922011. |
Composite | 1835 | Shuling Xiao | |
BBa_K2922021 | 109-kil-T7-cenA-T7-bgl1A | A composite part linked by BBa_K2922015 and the high level expression of beta-glucosidase by T7 promoter |
Composite | 3159 | Xv Jiang | |
BBa_K2922022 | 109-kil-T7-cex-T7-bgl1A | A composite part linked by BBa_K2922018 and the high level expression of beta-glucosidase by T7 promoter |
Composite | 3267 | Xv Jiang | |
BBa_K2922023 | 109-kil-T7-cex-T7-bgl1A-mRFP | BBa_K2623022 with mRFP reporter |
Composite | 4100 | Xv Jiang | |
BBa_K2922030 | TEimm | A composite part linked by BBa_K525998 and BBa_K2922025 |
Composite | 488 | Xingyu Chen | |
BBa_K2922031 | TEkil | A composite part linked by BBa_K525998 and BBa_K2922026 |
Composite | 284 | Xingyu Chen | |
BBa_K2922032 | TNimm | A composite part linked by BBa_K525998 and BBa_K2922028 |
Composite | 542 | Xingyu Chen | |
BBa_K2922033 | TNkil | A composite part linked by BBa_K525998 and BBa_K2922029 |
Composite | 305 | Yunyue Shi | |
BBa_K2922034 | TN | The colicin-N operon under T7 promoter control |
Composite | 1923 | Yunyue Shi | |
BBa_K2922035 | PN | The colicin-N operon under Arabinose promoter control |
Composite | 2021 | Yunyue Shi | |
BBa_K2922036 | TE | The colicin-E1 operon under T7 promoter control | Composite | 2247 | Man Yang | |
BBa_K2922036 | PE | The colicin-E1 operon under pBAD Arabinose promoter control | Composite | 2345 | Man Yang | |
BBa_K2922036 | TND | BBa_K2623034 with gfasPurple chromoprotein reporter | Composite | 2749 | Man Yang | |
BBa_K2922039 | PEF | BBa_K2623037 with amajLime chromoprotein reporter | Composite | 3195 | Gezhi Xiao | |
BBa_K2922042 | T7-CAV1 | The high level expression of BBa_K2922040 by T7 promoter |
Composite | 720 | Yuan Li | |
BBa_K2922043 | PbBD | Expression system of DsbA-PbBD coding periplasm display of lead binding peptide with T7 promoter |
Composite | 987 | Yuan Li | |
BBa_K2922044 | MBP-CAV1 | Co-expression system of periplasm display of lead binding peptide and caveolin-1 for enhancing lead absorption efficiency |
Composite | 1930 | Yuan Li |
This year, we have formulated a Part Collection related to secretion of intracellular proteins, which includes two major parts: one is secretion achieved with the help of YebF in the form of fusion proteins, the other is the expression of Kil protein by promoters of different intensities, thus changing cell permeability to different degrees and finally realizing secretion. For our Part Collection, the efficiency of three different intracellular proteins we utilized were well tested, recorded in detail and completely uploaded to the registry, which would be instructive in the field of design and characterization for many teams.
Register Name | Code used in Lab | Description | Types | Lenth |
---|---|---|---|---|
BBa_K2922000 | y-bgl1A | The protein beta-glucosidase with protein YebF fused to its N-terminus by GS Linker. |
Coding | 1764 |
BBa_K2922001 | y-cenA | The protein endoglucanase A with protein YebF fused to its N-terminus by GS Linker. |
Coding | 1731 |
BBa_K2922002 | y-cex | The protein exoglucanase with protein YebF fused to its N-terminus by GS Linker. |
Coding | 1839 |
BBa_K2922009 | 109-kil | The expression of Kil protein by J23109 promoter |
Composite | 248 |
BBa_K2922010 | 112-kil | The expression of Kil protein by J23112 promoter |
Composite | 248 |
BBa_K2922011 | 114-kil | The expression of Kil protein by J23114 promoter |
Composite | 248 |
BBa_K2922012 | 109-kil-T7-bgl1A | The secretion of beta-glucosidase by BBa_K2922009. |
Composite | 1760 |
BBa_K2922013 | 112-kil-T7-bgl1A | The secretion of beta-glucosidase by BBa_K2922010. |
Composite | 1760 |
BBa_K2922014 | 114-kil-T7-bgl1A | The secretion of beta-glucosidase by BBa_K2922011. |
Composite | 1760 |
BBa_K2922015 | 109-kil-T7-cenA | The secretion of endoglucanase by BBa_K2922009. |
Composite | 1727 |
BBa_K2922016 | 112-kil-T7-cenA | The secretion of endoglucanase by BBa_K2922010. |
Composite | 1727 |
BBa_K2922017 | 114-kil-T7-cenA | The secretion of endoglucanase by BBa_K2922011. |
Composite | 1727 |
BBa_K2922018 | 109-kil-T7-cex | The secretion of exoglucanase by BBa_K2922009. |
Composite | 1835 |
BBa_K2922019 | 112-kil-T7-cex | The secretion of exoglucanase by BBa_K2922010. |
Composite | 1835 |
BBa_K2922020 | 114-kil-T7-cex | The secretion of exoglucanase by BBa_K2922011. |
Composite | 1835 |