Team:XMU-China/Parts


This year, among the 41 parts designed, ten are basic parts and the other are composite ones. All of the parts are designed by ourselves, and most of them have been tested to insure their reliability. You can click here to learn more about our project to see their specific function, or click on the part numbers when browse website ( parts.igem.org ).

Among the ten basic parts designed this year by ourselves, BBa_K2922040 is our favorite one, which comes from human genomes. The gene enables engineering bacteria to form a vesicle which will have great applications in the enrichment of substrate (such as metal ions) through extracellular membrane and the enhancement of cell stress resistance. Besides, many other basic parts have also been designed to construct our project.


Favorite Register Name Code used in Lab Description Types Lenth Designer
R BBa_K2922040 CAV1 Caveolin-1 coding region Coding 537 Gezhi Xiao
BBa_K2922000 y-bgl1A The protein beta-glucosidase
 with protein YebF fused to its
 N-terminus by GS Linker.		 Coding 1764 Jiyang Zheng
BBa_K2922001 y-cenA The protein endoglucanase A
with protein YebF fused to its
N-terminus by GS Linker.		 Coding 1731 Junhong Chen
BBa_K2922002 y-cex The protein exoglucanase
with protein YebF fused to its
N-terminus by GS Linker. 		Coding 1839 Ruofan Yang
BBa_K2922024 Colicin-E1 Colicin-E1 coding region Coding 1569 Yi Lin
BBa_K2922025 Eimm Colicin-E1 immunity protein coding region Coding 342 Yi Lin
BBa_K2922026 Ekil Lysis protein for colicin-E1 coding region Coding 138 Yi Lin
BBa_K2922027 Colicin-N Colicin-N coding region Coding 1168 Huilin Guo
BBa_K2922028 Nimm Colicin-N immunity protein coding region Regulatory 396 Huilin Guo
BBa_K2922029 Nkil Lysis protein for colicin-N
coding region		 Coding 159 Huilin Guo

A series of composite parts have been designed this year, and most of them are useful for our project. And our favorite composite part is BBa_K2922009 , which contains the sequence of the protein Kil. Kil protein, which accumulates in the periplasmic space of the bacteria, can increase the periplasmic space and improved the membrane permeability of bacteria. Therefore, the protein inside the bacteria can secrete out of bacteria with the help from Kil protein. The use of proper intensity of the promoter can not only achieve the purpose of secretion but also prevent side effects of cell lysis and death. After a series of experiments, J23109 is determined as the most proper promoter.


Favorite Register Name Code used in Lab Description Types Lenth Designer
R BBa_K2922009 109-kil The expression of Kil protein
by J23109 promoter		 Composite 248 Fude Chen
BBa_K2922006 T7-y-bgl1A The high level secretion 
of  BBa_K2922000
by T7 promoter		 Composite 1882 Tianyu Feng
BBa_K2922007 T7-y-cenA The high level expression 
of  BBa_K2922001
by T7 promoter		 Composite 1849 Tianyu Feng
BBa_K2922008 T7-y-cex The high level expression
 of  BBa_K2922002
by T7 promoter		 Composite 1957 Tianyu Feng
BBa_K2922010 112-kil The expression of Kil protein
by J23112 promoter		 Composite 248 Fude Chen
BBa_K2922011 114-kil The expression of Kil protein
by J23114 promoter		 Composite 248 Fude Chen
BBa_K2922012 109-kil-T7-bgl1A The secretion of 
beta-glucosidase
 by BBa_K2922009.		 Composite 1760 Jisheng Xie
BBa_K2922013 112-kil-T7-bgl1A The secretion of 
beta-glucosidase
 by BBa_K2922010.		 Composite 1760 Jisheng Xie
BBa_K2922014 114-kil-T7-bgl1A The secretion of 
beta-glucosidase
 by BBa_K2922011.		 Composite 1760 Jisheng Xie
BBa_K2922015 109-kil-T7-cenA The secretion of endoglucanase
 by BBa_K2922009.		 Composite 1727 Zinuo Huang
BBa_K2922016 112-kil-T7-cenA The secretion of endoglucanase
 by BBa_K2922010.		 Composite 1727 Zinuo Huang
BBa_K2922017 114-kil-T7-cenA The secretion of endoglucanase
 by BBa_K2922011.		 Composite 1727 Zinuo Huang
BBa_K2922018 109-kil-T7-cex The secretion of exoglucanase
 by BBa_K2922009.		 Composite 1835 Shuling Xiao
BBa_K2922019 112-kil-T7-cex The secretion of exoglucanase
 by BBa_K2922010.		 Composite 1835 Shuling Xiao
BBa_K2922020 114-kil-T7-cex The secretion of exoglucanase
 by BBa_K2922011.		 Composite 1835 Shuling Xiao
BBa_K2922021 109-kil-T7-cenA-T7-bgl1A A composite part linked
by BBa_K2922015 and
the high level expression 
of beta-glucosidase
by T7 promoter 		Composite 3159 Xv Jiang
BBa_K2922022 109-kil-T7-cex-T7-bgl1A A composite part linked
by BBa_K2922018 and
the high level expression
 of beta-glucosidase
by T7 promoter 		Composite 3267 Xv Jiang
BBa_K2922023 109-kil-T7-cex-T7-bgl1A-mRFP BBa_K2623022 with
mRFP reporter		 Composite 4100 Xv Jiang
BBa_K2922030 TEimm A composite part linked
by BBa_K525998
and BBa_K2922025		 Composite 488 Xingyu Chen
BBa_K2922031 TEkil A composite part linked
by BBa_K525998
and BBa_K2922026		 Composite 284 Xingyu Chen
BBa_K2922032 TNimm A composite part linked
by BBa_K525998
and BBa_K2922028		 Composite 542 Xingyu Chen
BBa_K2922033 TNkil A composite part linked
by BBa_K525998
and BBa_K2922029		 Composite 305 Yunyue Shi
BBa_K2922034 TN The colicin-N operon under
T7 promoter control		 Composite 1923 Yunyue Shi
BBa_K2922035 PN The colicin-N operon under
Arabinose promoter control		 Composite 2021 Yunyue Shi
BBa_K2922036 TE The colicin-E1 operon under T7 promoter control Composite 2247 Man Yang
BBa_K2922036 PE The colicin-E1 operon under pBAD Arabinose promoter control Composite 2345 Man Yang
BBa_K2922036 TND BBa_K2623034 with gfasPurple chromoprotein reporter Composite 2749 Man Yang
BBa_K2922039 PEF BBa_K2623037 with amajLime chromoprotein reporter Composite 3195 Gezhi Xiao
BBa_K2922042 T7-CAV1 The high level expression
 of BBa_K2922040
by T7 promoter		 Composite 720 Yuan Li
BBa_K2922043 PbBD Expression system of
DsbA-PbBD coding periplasm
display of lead binding peptide
with T7 promoter 		Composite 987 Yuan Li
BBa_K2922044 MBP-CAV1 Co-expression system of
periplasm display of lead
binding peptide and caveolin-1
for enhancing lead
absorption efficiency		 Composite 1930 Yuan Li

This year, we have formulated a Part Collection related to secretion of intracellular proteins, which includes two major parts: one is secretion achieved with the help of YebF in the form of fusion proteins, the other is the expression of Kil protein by promoters of different intensities, thus changing cell permeability to different degrees and finally realizing secretion. For our Part Collection, the efficiency of three different intracellular proteins we utilized were well tested, recorded in detail and completely uploaded to the registry, which would be instructive in the field of design and characterization for many teams.


Register Name Code used in Lab Description Types Lenth
BBa_K2922000 y-bgl1A The protein beta-glucosidase
 with protein YebF fused to its
 N-terminus by GS Linker.		 Coding 1764
BBa_K2922001 y-cenA The protein endoglucanase A
with protein YebF fused to its
N-terminus by GS Linker.		 Coding 1731
BBa_K2922002 y-cex The protein exoglucanase
with protein YebF fused to its
N-terminus by GS Linker. 		Coding 1839
BBa_K2922009 109-kil The expression of Kil protein
by J23109 promoter		 Composite 248
BBa_K2922010 112-kil The expression of Kil protein
by J23112 promoter		 Composite 248
BBa_K2922011 114-kil The expression of Kil protein
by J23114 promoter		 Composite 248
BBa_K2922012 109-kil-T7-bgl1A The secretion of 
beta-glucosidase
 by BBa_K2922009.		 Composite 1760
BBa_K2922013 112-kil-T7-bgl1A The secretion of 
beta-glucosidase
 by BBa_K2922010.		 Composite 1760
BBa_K2922014 114-kil-T7-bgl1A The secretion of 
beta-glucosidase
 by BBa_K2922011.		 Composite 1760
BBa_K2922015 109-kil-T7-cenA The secretion of endoglucanase
 by BBa_K2922009.		 Composite 1727
BBa_K2922016 112-kil-T7-cenA The secretion of endoglucanase
 by BBa_K2922010.		 Composite 1727
BBa_K2922017 114-kil-T7-cenA The secretion of endoglucanase
 by BBa_K2922011.		 Composite 1727
BBa_K2922018 109-kil-T7-cex The secretion of exoglucanase
 by BBa_K2922009.		 Composite 1835
BBa_K2922019 112-kil-T7-cex The secretion of exoglucanase
 by BBa_K2922010.		 Composite 1835
BBa_K2922020 114-kil-T7-cex The secretion of exoglucanase
 by BBa_K2922011.		 Composite 1835

Xiamen University, Fujian, China

No.422, Siming South Road, Fujian, P.R.China 361005