Team:XMU-China/Part Collection
This year, we have formulated a Part Collection related to secretion of intracellular proteins, which includes two major parts: one is secretion achieved with the help of YebF in the form of fusion proteins, the other is the expression of Kil protein by promoters of different intensities, thus changing cell permeability to different degrees and finally realizing secretion. For our Part Collection, the efficiency of three different intracellular proteins we utilized were well tested, recorded in detail and completely uploaded to the registry, which would be instructive in the field of design and characterization for many teams.
| Register Name | Code used in Lab | Description | Types | Lenth |
|---|---|---|---|---|
| BBa_K2922000 | y-bgl1A | The protein beta-glucosidase with protein YebF fused to its N-terminus by GS Linker. |
Coding | 1764 |
| BBa_K2922001 | y-cenA | The protein endoglucanase A with protein YebF fused to its N-terminus by GS Linker. |
Coding | 1731 |
| BBa_K2922002 | y-cex | The protein exoglucanase with protein YebF fused to its N-terminus by GS Linker. |
Coding | 1839 |
| BBa_K2922009 | 109-kil | The expression of Kil protein by J23109 promoter |
Composite | 248 |
| BBa_K2922010 | 112-kil | The expression of Kil protein by J23112 promoter |
Composite | 248 |
| BBa_K2922011 | 114-kil | The expression of Kil protein by J23114 promoter |
Composite | 248 |
| BBa_K2922012 | 109-kil-T7-bgl1A | The secretion of beta-glucosidase by BBa_K2922009. |
Composite | 1760 |
| BBa_K2922013 | 112-kil-T7-bgl1A | The secretion of beta-glucosidase by BBa_K2922010. |
Composite | 1760 |
| BBa_K2922014 | 114-kil-T7-bgl1A | The secretion of beta-glucosidase by BBa_K2922011. |
Composite | 1760 |
| BBa_K2922015 | 109-kil-T7-cenA | The secretion of endoglucanase by BBa_K2922009. |
Composite | 1727 |
| BBa_K2922016 | 112-kil-T7-cenA | The secretion of endoglucanase by BBa_K2922010. |
Composite | 1727 |
| BBa_K2922017 | 114-kil-T7-cenA | The secretion of endoglucanase by BBa_K2922011. |
Composite | 1727 |
| BBa_K2922018 | 109-kil-T7-cex | The secretion of exoglucanase by BBa_K2922009. |
Composite | 1835 |
| BBa_K2922019 | 112-kil-T7-cex | The secretion of exoglucanase by BBa_K2922010. |
Composite | 1835 |
| BBa_K2922020 | 114-kil-T7-cex | The secretion of exoglucanase by BBa_K2922011. |
Composite | 1835 |