Team:XMU-China/Composite Part
A series of composite parts have been designed this year, and most of them are useful for our project. And our favorite composite part is BBa_K2922009 , which contains the sequence of the protein Kil. Kil protein, which accumulates in the periplasmic space of the bacteria, can increase the periplasmic space and improved the membrane permeability of bacteria. Therefore, the protein inside the bacteria can secrete out of bacteria with the help from Kil protein. The use of proper intensity of the promoter can not only achieve the purpose of secretion but also prevent side effects of cell lysis and death. After a series of experiments, J23109 is determined as the most proper promoter.
| Favorite | Register Name | Code used in Lab | Description | Types | Lenth | Designer |
|---|---|---|---|---|---|---|
| R | BBa_K2922009 | 109-kil | The expression of Kil protein by J23109 promoter |
Composite | 248 | Fude Chen |
| BBa_K2922006 | T7-y-bgl1A | The high level secretion of BBa_K2922000 by T7 promoter |
Composite | 1882 | Tianyu Feng | |
| BBa_K2922007 | T7-y-cenA | The high level expression of BBa_K2922001 by T7 promoter |
Composite | 1849 | Tianyu Feng | |
| BBa_K2922008 | T7-y-cex | The high level expression of BBa_K2922002 by T7 promoter |
Composite | 1957 | Tianyu Feng | |
| BBa_K2922010 | 112-kil | The expression of Kil protein by J23112 promoter |
Composite | 248 | Fude Chen | |
| BBa_K2922011 | 114-kil | The expression of Kil protein by J23114 promoter |
Composite | 248 | Fude Chen | |
| BBa_K2922012 | 109-kil-T7-bgl1A | The secretion of beta-glucosidase by BBa_K2922009. |
Composite | 1760 | Jisheng Xie | |
| BBa_K2922013 | 112-kil-T7-bgl1A | The secretion of beta-glucosidase by BBa_K2922010. |
Composite | 1760 | Jisheng Xie | |
| BBa_K2922014 | 114-kil-T7-bgl1A | The secretion of beta-glucosidase by BBa_K2922011. |
Composite | 1760 | Jisheng Xie | |
| BBa_K2922015 | 109-kil-T7-cenA | The secretion of endoglucanase by BBa_K2922009. |
Composite | 1727 | Zinuo Huang | |
| BBa_K2922016 | 112-kil-T7-cenA | The secretion of endoglucanase by BBa_K2922010. |
Composite | 1727 | Zinuo Huang | |
| BBa_K2922017 | 114-kil-T7-cenA | The secretion of endoglucanase by BBa_K2922011. |
Composite | 1727 | Zinuo Huang | |
| BBa_K2922018 | 109-kil-T7-cex | The secretion of exoglucanase by BBa_K2922009. |
Composite | 1835 | Shuling Xiao | |
| BBa_K2922019 | 112-kil-T7-cex | The secretion of exoglucanase by BBa_K2922010. |
Composite | 1835 | Shuling Xiao | |
| BBa_K2922020 | 114-kil-T7-cex | The secretion of exoglucanase by BBa_K2922011. |
Composite | 1835 | Shuling Xiao | |
| BBa_K2922021 | 109-kil-T7-cenA-T7-bgl1A | A composite part linked by BBa_K2922015 and the high level expression of beta-glucosidase by T7 promoter |
Composite | 3159 | Xv Jiang | |
| BBa_K2922022 | 109-kil-T7-cex-T7-bgl1A | A composite part linked by BBa_K2922018 and the high level expression of beta-glucosidase by T7 promoter |
Composite | 3267 | Xv Jiang | |
| BBa_K2922023 | 109-kil-T7-cex-T7-bgl1A-mRFP | BBa_K2623022 with mRFP reporter |
Composite | 4100 | Xv Jiang | |
| BBa_K2922030 | TEimm | A composite part linked by BBa_K525998 and BBa_K2922025 |
Composite | 488 | Xingyu Chen | |
| BBa_K2922031 | TEkil | A composite part linked by BBa_K525998 and BBa_K2922026 |
Composite | 284 | Xingyu Chen | |
| BBa_K2922032 | TNimm | A composite part linked by BBa_K525998 and BBa_K2922028 |
Composite | 542 | Xingyu Chen | |
| BBa_K2922033 | TNkil | A composite part linked by BBa_K525998 and BBa_K2922029 |
Composite | 305 | Yunyue Shi | |
| BBa_K2922034 | TN | The colicin-N operon under T7 promoter control |
Composite | 1923 | Yunyue Shi | |
| BBa_K2922035 | PN | The colicin-N operon under Arabinose promoter control |
Composite | 2021 | Yunyue Shi | |
| BBa_K2922036 | TE | The colicin-E1 operon under T7 promoter control | Composite | 2247 | Man Yang | |
| BBa_K2922036 | PE | The colicin-E1 operon under pBAD Arabinose promoter control | Composite | 2345 | Man Yang | |
| BBa_K2922036 | TND | BBa_K2623034 with gfasPurple chromoprotein reporter | Composite | 2749 | Man Yang | |
| BBa_K2922039 | PEF | BBa_K2623037 with amajLime chromoprotein reporter | Composite | 3195 | Gezhi Xiao | |
| BBa_K2922042 | T7-CAV1 | The high level expression of BBa_K2922040 by T7 promoter |
Composite | 720 | Yuan Li | |
| BBa_K2922043 | PbBD | Expression system of DsbA-PbBD coding periplasm display of lead binding peptide with T7 promoter |
Composite | 987 | Yuan Li | |
| BBa_K2922044 | MBP-CAV1 | Co-expression system of periplasm display of lead binding peptide and caveolin-1 for enhancing lead absorption efficiency |
Composite | 1930 | Yuan Li |