Difference between revisions of "Team:CSMU Taiwan/Collaborations"
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<img src="https://static.igem.org/mediawiki/2019/3/3e/T--CSMU_Taiwan--ccu1.jpeg" alt="“figure 1" in case image doesn’t appear style="width:20% height: auto;"> | <img src="https://static.igem.org/mediawiki/2019/3/3e/T--CSMU_Taiwan--ccu1.jpeg" alt="“figure 1" in case image doesn’t appear style="width:20% height: auto;"> | ||
<u>Online meeting with CCU iGEM team</u> | <u>Online meeting with CCU iGEM team</u> | ||
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<img src="https://static.igem.org/mediawiki/2019/6/67/T--CSMU_Taiwan--ccu4.jpeg" in case image doesn’t appear style="width:20% height: auto;"> | <img src="https://static.igem.org/mediawiki/2019/6/67/T--CSMU_Taiwan--ccu4.jpeg" in case image doesn’t appear style="width:20% height: auto;"> | ||
<u>Meep up with CCU iGEM team</u> | <u>Meep up with CCU iGEM team</u> | ||
Revision as of 10:32, 20 October 2019
iGEM 2019 Taiwan conference
On August 5th , most Taiwan IGEM team got together in Taichung ,thanks to NCKU IGEM team. We presented and showed poster to each other. We challenged each others’ project and learned a lot from each others. We realized the pros and cons about our project, so we optimize our project after the conference. Last but not least, we became friends.
Presentation in iGEM 2019 Taiwan conference
CCU iGEM team
We wanted to manufacture our rapid diagnostic test, but we didn’t have relevant equipments and techniques. We asked CCU IGEM team for help. We shared our idea and showed them a detailed drawing of the device. They analyzed the design and gave us some advices based on manufacturing difficulties and structural integrity. After confirming all the details, they produced 11 sets of the device made of PLA using 3D printers and delivered to us, so that we could put our strip inside to use and demonstrate our product! Besides, we shared some ideas as well as suggestions about characterization to them. Since we performed SELEX, we had conducted PCR for many times. We gave some advices about their sequences, such as adding biotin to one strand of double strand DNA ,so that they could separate their double strand DNA.
Online meeting with CCU iGEM team
Meep up with CCU iGEM team
The rapid detection device made by CCU iGEM team
We are joyful and thankful while receiving the device.
Mingdao iGEM team
This year, team Mingdao aimed to produce carbonic anhydrase (CA) which enhanced CO2 dissolving rate, as well as CYP2E1 to break down chloroform and benzene. We helped Mingdao with purifying carbonic anhydrase from E.coli and protein quantitation. We enabled them to estimate the yield of their proteins in a single production, and predict the efficacy of their products. What’s more, we also invited Mingdao iGEM team to our lab and teached them how to perform over-expression, purification, dialysis and ELISA with our protocol. At mid-July, we had problems doing TA cloning.We asked team Mingdao for help,since they are the experts of TA cloning and gene cloning. They successfully inserted our aptamers into plasmid, and reminded us the tips. After that, we successfully performed TA cloning .
We went to team Mingdao's lab.
We invited Mingdao iGEM team to our lab and teached them how to perform over-expression, purification, dialysis and ELISA
The result of TA cloning helped by Mingdao
The result of over-expression in SDS page that we helped Mingdao
The result of purification in SDS page that we helped Mingdao
The result of purification in Western blot that we helped Mingdao
Tunghai_TAPG
It’s Tunghai_TAPG’s first time participating in iGEM .We had a great meet up with each other. We gave some advices for them, and they also gave us some awesome ideas. After the meep up, we had a tight connection between each other. We used ultrasonic reactor to dissolve E.coli and helped them make sure their polypeptide’s molecular mass.Then, we told them some tips and gave them our protocol, so that they could purify by themselves.
Meet up with Tunghai_TAPG iGEM team
The result of over-expression in SDS page that we helped Tunghai_TAPG
The draft of the swab tube
NCHU iGEM team
We had several meet up with NCHU iGEM team, we shared ideas with each other. We suggested NCHU iGEM team to add tag on the sequence so that they could purify their proteins and estimated the efficiency of each step of their whole system. Besides, NCHU iGEM suggested that we could use mass spectrometer to perform Qualitative and quantitative analysis.
Online meeting with NCHU iGEM team
Meet up with NCHU iGEM team
iGEM Aptamers Hub
Collaborating Teams: iGEM Athens, iGEM Madrid, iGEM Pasteur-Paris, iGEM Rotterdam, iGEM Strasbourg, iGEM BOKU-Vienna, iGEM Cornell, iGEM CSMU Taiwan, iGEM DUT China A
While conducting our project, we wished many times for there to be a more formal platform for people working with aptamers to share ideas, advice, and feedback. For this reason, most of the teams working with aptamers this year created the iGEM Aptamers Hub. The iGEM Aptamers Hub aimed to enable the use of aptamers during the iGEM competition and encourage their use in laboratories worldwide. We created an ‘‘aptamer registry’’, created a network with aptamer researchers, and provided the community with useful resources to facilitate their research. We offered our own way about selecting aptamers, analyzed its pros and cons, communicated with other teams, and shared ideas with the public. We held group discussions through Skype and WhatsApp in order to decide on important issues concerning the Hub’s organization and function. We shared our own methods about selecting aptamers with others, and received many useful advices from the online meeting. For example, we got the information about RNA-puzzles, so that we could stimulate the aptamers’ 3D structure.
