Difference between revisions of "Team:SMMU-China/Software"
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<div class="medium_framework"> | <div class="medium_framework"> | ||
| − | <h1> | + | <h1>FIRST PART: The design ideas</h1> |
| − | <p> | + | <p>After we have designed the core component. When the receptor on core cells surfaces bind to the antigen of cancer cells surfaces. It can initiate a downstream signaling pathway, which could activate DNA, we transfected using plasmid or phage, transcription into mRNA. Then the mRNA through the nuclear pore into the cytoplasm translated into the signal molecules we needed (generally some interleukins, such as IL-15). Signal molecules finally secreting into the extracellular fluid into the blood. If we want to detect the released signal molecules, the only way is to puncture skin and take the peripheral blood for detection. Although this method can quickly determine the existence and concentration of the signal molecules, also has its shortage such as, must to collecting the peripheral blood, can not continuous monitoring the concentration and need to go to the professional medical institution to operate, etc.</p> |
| + | <p>In our extension part we have designed another engineering cell which could bind to the signal molecules mentioned above, and then express the fluorescent protein floating in cytoplasm. It could bind with the different type of signal molecules and the corresponding cell initiated in order to distinguish the dominant tumor cell population. So that we could detect the optical signal to determine the concentration of molecules.</p> | ||
| + | <p>Our method allows us to monitor the signal molecules expressed by our engineered cells in real time without collecting peripheral blood so that we can monitor cancer development simply and dynamically.</p> | ||
</div> | </div> | ||
<div class="medium_framework"> | <div class="medium_framework"> | ||
| − | <h1> | + | <h1>SECOND PART: Principle of the software and modeling</h1> |
| − | <p> | + | <p><b>The principle flow chart is as follows:</b></p> |
| + | <img src="https://static.igem.org/mediawiki/2019/c/cf/T--SMMU-China--software_1.png" alt="smmu_software_1" style="margin: 10px 0px;"> | ||
| + | |||
</div> | </div> | ||
Revision as of 08:41, 18 October 2019
FIRST PART: The design ideas
After we have designed the core component. When the receptor on core cells surfaces bind to the antigen of cancer cells surfaces. It can initiate a downstream signaling pathway, which could activate DNA, we transfected using plasmid or phage, transcription into mRNA. Then the mRNA through the nuclear pore into the cytoplasm translated into the signal molecules we needed (generally some interleukins, such as IL-15). Signal molecules finally secreting into the extracellular fluid into the blood. If we want to detect the released signal molecules, the only way is to puncture skin and take the peripheral blood for detection. Although this method can quickly determine the existence and concentration of the signal molecules, also has its shortage such as, must to collecting the peripheral blood, can not continuous monitoring the concentration and need to go to the professional medical institution to operate, etc.
In our extension part we have designed another engineering cell which could bind to the signal molecules mentioned above, and then express the fluorescent protein floating in cytoplasm. It could bind with the different type of signal molecules and the corresponding cell initiated in order to distinguish the dominant tumor cell population. So that we could detect the optical signal to determine the concentration of molecules.
Our method allows us to monitor the signal molecules expressed by our engineered cells in real time without collecting peripheral blood so that we can monitor cancer development simply and dynamically.
SECOND PART: Principle of the software and modeling
The principle flow chart is as follows:
DEMO PART 3
Your team has been approved and you are ready to start the iGEM season! Your team has been approved and you are ready to start the iGEM season! Your team has been approved and you are ready to start the iGEM season! Your team has been approved and you are ready to start the iGEM season! Your team has been approved and you are ready to start the iGEM season! Your team has been approved and you are ready to start the iGEM season! Your team has been approved and you are ready to start the iGEM season!