Line 331: | Line 331: | ||
<h3>header</h3> | <h3>header</h3> | ||
<h3>Sequence selection and Codon Optimization</h4> | <h3>Sequence selection and Codon Optimization</h4> | ||
− | <p>The previous part (BBa_K1955000) was documented to be acquired from NCBI with no further indication. The sequence of HA strain(A/WSN 1933/TS61(H1N1)) is based on <a href= | + | <p>The previous part (BBa_K1955000) was documented to be acquired from NCBI with no further indication. The sequence of HA strain(A/WSN 1933/TS61(H1N1)) is based on <a href="https://www.ncbi.nlm.nih.gov/nucleotide/CY010788.1?report=genbank&log$=nuclalign&blast_rank=2&RID=F439K4B5015%22%20target=%22blank%22">NCBI(CY010788.1)</a>, being 7 bp difference to the precious part(Fig.1). </p> |
<p>To express the target protein, we chose to use BL21 E.coli strain with a pET29a vector. In order to improve the expression efficiency of our chassis, we first codon optimized the sequence and checked for illegal cutting sites using ATGme for E.coli. </p> | <p>To express the target protein, we chose to use BL21 E.coli strain with a pET29a vector. In order to improve the expression efficiency of our chassis, we first codon optimized the sequence and checked for illegal cutting sites using ATGme for E.coli. </p> | ||
Revision as of 15:49, 16 October 2019
![](https://static.igem.org/mediawiki/2019/thumb/c/ca/T--CSMU_Taiwan--gene_red.png/613px-T--CSMU_Taiwan--gene_red.png)
![Avatar](https://static.igem.org/mediawiki/2019/8/88/T--CSMU_Taiwan--student3-1.png)
Sequence Optimization
TEV Cutting Site
Further Applications
Improved Solubility
header
Sequence selection and Codon Optimization
The previous part (BBa_K1955000) was documented to be acquired from NCBI with no further indication. The sequence of HA strain(A/WSN 1933/TS61(H1N1)) is based on NCBI(CY010788.1), being 7 bp difference to the precious part(Fig.1).
To express the target protein, we chose to use BL21 E.coli strain with a pET29a vector. In order to improve the expression efficiency of our chassis, we first codon optimized the sequence and checked for illegal cutting sites using ATGme for E.coli.