Team:iBowu-China
Potatoes are the fourth largest crops in the world. Soft rot is one of the main plant diseases for the decrease of potato production. Pectobacterium carotovorum (Erwinia carotovorum) is a main plant pathogen causing soft rot of potatoes as well as many other crops.
In our new method, Cell-Free system in bio-control had become the potential key to the next-generation agriculture and it represents a promising alternative to fight against plant pathogens, which is an even more eco-friendly option compared to the bio-pesticides.
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In our new method, Cell-Free system in bio-control had become the potential key to the next-generation agriculture and it represents a promising alternative to fight against plant pathogens, which is an even more eco-friendly option compared to the bio-pesticides.
For more information, please click on the icon
We designed two kinds of gene circuits in order to detect and prevent the disease. One’s function is to detect AHL (quorum sensing signal molecular) of P. carotovorum; the other is to express the hydrolase AiiA and the antibacterial peptides which could degrade AHL and generally kill bacteria separately.For more information, please click on the icon
We designed a simple mathematical model based on the differential equations that describe the biochemical processes of the cell-free system.The model can be used to predict the minimum detection concentration of AHL and optimize the DNA content.For more information, please click on the icon
With the project divided into three stages (pre-project research, project modification, and interview with investors), the human practice team targeted specific events in each stage. During different stages, the team focused on various potato related issues that affects both the society and farming industry.
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Next generation bio-pesticide
By combining genetic engineering methods with cell-free synthesis, we are able to create a general solution for a variety of crop bacterial diseases. Diagnosing is the most important but also a very difficult step during the process of disease control, it requires a lot of expertise and equipments. With the help of synthetic biology, we’re able to use bacterial quorum sensing to detect bacterial population and diagnosis disease in a simpler way, and lastly, Cell-free protein synthesis free us from the biosafety problem.