Team:WHU-China/Attributions




Attribution




GENERAL SUPPORT




Wuhan University provides the opportunity for us to attend iGEM. And the comprehensive of Wuhan university makes our team a interdisciplinary one. Our team members come from College of life Sciences, Hongyi Honor College, School of Mathematics and Statisitics and School of Computer Sciences.





PROJECT SUPPORT AND ADVISER




PI





We appreciate our PI a lot for they gave us a lot of supporting and suggestion on our projects. When we had some new ideas they always encouraged us to have a try and helped us to achieve them. At the same time, they left enough space for ourselves to complete our own project which let us learn a lot from this experience.




Experts of museums






To complete our project, we paid visits to a lot of museums and interviewed several experts in silk relics restoration. From them we got a lot of improvements suggesting and a certain amount of approval. You can see specific attribution in Integrated HP.



Theory support




  • Tomoya Imai et al. Functional Reconstitution of Cellulose Synthase in Escherichia coli. Biomac. -- sending us the plasmids of BC production, helping as achieve the BC production in E.coli.

  • Mark R. Bleackley et al.Nicotiana alata?Defensin Chimeras Reveal Differences in the Mechanism of Fungal and Tumor Cell Killing and an Enhanced Antifungal Variant.Antimicrob Agents Chemother. 2016 Oct; 60(10): 6302–6312. -- Providing us the information of the AMP NaD2.

  • Pere Picart et al.Identification of defensin-encoding genes of?Picea glauca: characterization of PgD5, a conserved spruce defensin with strong antifungal activity.BMC Plant Biol. 2012; 12: 180. -- providing us the information of the AMP PgD5.

  • Yanyan Wu et al.Preparation and Antioxidant Activities In Vitro of a Designed Antioxidant Peptide from Pinctada fucata by Recombinant Escherichia coli.J. Microbiol. Biotechnol. (2018), 28(1), 1–11 -- providing us the information of the DSAOP.

  • Nicholas T. Ong et al.Engineering an E. coli Near-Infrared Light Sensor. Acs synthetic Biology. -- providing us the idea of near infrared light promoter.

  • Sunqing Wu et al.细菌纤维素加固古代糟朽丝织品及光老化特征研究. -- providing us the inspiration of reinforcement material and knowledge about silk relics’ conservation.

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Other Advisers




  • Prof. Lijia Li -- helping us to solve problems on gene engineering design.

  • Prof. Hua Tong -- helping us to contact with expert Wu, and providing us information about BC reinforcement.

  • Prof. Xiaofeng Tang -- helping us to solve the problem of Chloramphenicol failure and giving our project advice.

  • Prof. Xiangdong Gao -- giving our project advice.

  • Prof. Qingzhen Liu -- giving our molecular mechanism knowledge.

  • Prof. Zhijian Cao -- giving suggestion on AMP production and purification.

  • Associate Prof. Fang Peng -- providing us strains and equipment for high fracturing bacteria.

  • Associate Prof. Yuping Huang -- giving our information about inoculation of G.xylinus.

  • Senior iGEMer Tianyi Chang, Renjie Zhou, Yue Qing, Yuyan Liu -- giving us help among all kinds of aspects of our project.

  • Dorothy Zhang -- help us to understand the rules of iGEM.




LAB SUPPORT





Schools




  • College of Life Science, Wuhan University -- Provide us with a science training lab to conduct all the molecular biology experiment.

  • School of Physics and Technology, Wuhan University -- Provide us universal?tester to achieve our silk performance testing.




Teachers and predecessors




  • Teacher Yan Long, Researcher Yuanming Yang, Teacher Jing Che, Teacher Xiaoyan Li -- providing us adequate experimental conditions and helping us to solve all kinds of problems during our experiments.

  • Postgraduate student Qianyun Liu, Yingjian Li, Shiyu Teng, Yongping Zhang -- teaching us some experimental skills and helping us to solve experimental problems.




ORGANIZATION SUPPORT




  • GenScript -- Help us do DNA synthesis.


  • Sangon Biotech, AuGCT company -- Help us do DNA synthesis, primer design and DNA sequencing.

  • CCTCC -- providing us molds strain and G.xylinus for experiments.


HP SUPPORT





These museums provided us the opportunities of visiting and interviewing, which gave a lot of inspirations to our project.





iGEMer SUPPORT





We appreciated these iGEM teams a lot for some of them providing a lot of improvement advice for our project and some of them gave us experimental help. We made good friends with these teams in iGEM competition! You can learn specific help in collaboration.





Other




The most important appreciation was for our teammates, everyone in our team made efforts in this project !