Team:USP-Brazil/Parts
Parts
For the _genSwicht project, we have assembled 3 promoters (pLac, pBAD and pT3) to a ratiometric characterization vector (BBa_K2771020), in order to compare the induction by different sources (IPTG, L-Arabinose and Blue light). For the proper function of the blue light promoter, we have built a new plasmid carrying every component related to light sensing at 450 nm (Part:BBa_K3095003).
Our work also involved the construction of two genetic circuit, approach A and approach B. The approach B of our project resulted in a new composite part (BBa_K3095011), this circuit was made to test the repression loop using the light responsive promoter, as it is described in Design. Finally, we also characterized the new part mTagBFP2, which encodes for a blue fluorescent protein.
USP-Brazil
