Team:UPNAvarra Spain/Notebook

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Notebook

July

Week 1 : 1st ⇾ 7th

In the beginning, we received the theorical and practical training required to develop our project and to get on well in the laboratory. We also made the most of that week to become familiar with the lab equipment. We also prepared culture media and started working with different plasmids.

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Week 2 : 8th ⇾ 14th

Due to the celebration of the San Fermin festival in Pamplona, our lab was closed for holidays. Therefore, we spent this week getting strength to continue with our project.

Week 3 : 15th ⇾ 21th

We came back to our daily routine. This time we focused our efforts on digestions, ligations and colony PCR. After several attempts we finally obtained a significant and different progress for each construction.

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Week 4 : 22th ⇾ 28th

Once to this point, each construction was at a different stage. By the end of the week the nitrate constructions were nearly finished. However, the remaining constructions were at different points in the cloning process.

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August

Week 5 : 29th ⇾ 4th

It was our jinx week, when nothing seemed to work. But we finally found our mistakes and we were able to deal with the problem. At the end we had some positive results and the work carried on.

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Week 6 : 5th ⇾ 11th

During this week we follow cloning our constructions and we started working on the induction of bacterial cultures.

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Week 7 : 12th ⇾ 18th

We finished some of our constructions and we sent them to sequencing. With those results we could confirm that some fragments corresponding to RBS were missing in the composite construction, so we design a strategy to solve this problem and finish the constructions on time.
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Week 8 : 19th ⇾ 26th

This week we received some parts kindly provided by Twist Bioscience. We want to thanked Twist for this contribution to our project. We used these fragments to obtain final composite parts which will make up our biosensor collection. At the same time, we performed some inductions to start characterizing our biosensors and tested their ability to detect nitrate, cooper, mercury or cadmium by the expression of different chromoproteins.

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Week 9 : 27th ⇾ 31th

We spent the last lab week repeating inductions to adjust the assayed concentrations and induction times, and also taking pictures that would be used in the following modeling process. In addition, we have dedicated one day to pick up water samples from river basins and agro-industrial activities to validate our theorical model.

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