Team:UA Huntsville/Experiments
Research
Protocols
Workshop 1 Handout Basic protocols
Basic ProtocolsPouring Plate and Culturing Protocols:
Pouring Plate and Culturing ProtocolsTransformation Protocols
Transformation ProtocolsAlkaline Lysis Mini Prep Protocols:
Alkaline Lysis Mini Prep ProtocolsPCR Protocol PCR protocols were conducted as listed on NEB website:
PCR ProtocolLitigation Protocols Litigation followed protocols as listed by New England Biolabs. Instead of masses listed, 0.02pmol vector and 0.06 pmol insert were used to fine-tune to each vector and insert. Protocol may be found at the following link:
Litigation ProtocolsExperiments
Experiment description can be found in the following pdf:
Experiments