Description: Ribosome binding site (BBa_B0034) joined with codon-optimized and improved Dsup (BBa_K2195000). A simple RBS-Dsup fragment that was inserted into BBa_K3097001. Used to gain radiation resistance as well as red fluorescence

Part BBA_K3097001

Registry: http://parts.igem.org/wiki/index.php?title=Part:BBa_K3097000

Description: Plasmid with promoter, RBS, gene of interest, coupler, fluorescent protein, and a terminator. Promoter allows transcription to start, RBS gives ribosome place to bind. Dsup was taken from tardigrades to confer increased radiation resistance to bacteria of interest. A coupler was added to avoid steric hindrance between different parts of the plasmid. RFP was added in order to test whether plasmid was inserted correctly and for differentiation purposes. A double terminator was added to stop transcription

Team:UA Huntsville/Contribution

Contributions

Part BBa_K3097000

Part BBA_K3097001