| Date |
Project fortnightly |
| 02/17-03/02 |
- Wet Lab: Designed the vector(TAPG)
- Dry Lab: The members were organized and everyone expressed their views on the project.
- Human practice: Browsing the previous iGEM wiki, and brainstorming about our team project
|
| 03/03-03/16 |
- Wet Lab: Designed the primers(eGFP、SUMO、6x His tag、intein)
- Dry Lab: Because our product is based on antimicrobial peptide as the main component of hospital’s supply, we need to detail the relationship between the various pointers of the environment in the hospital and the growth of bacteria, such as the temperature and humidity in the influence on bacterial growth and the effect of peptide.
- Human practice: Brainstorming possible outreach activities, including a science camp, high school lecture and volunteer etc.
|
| 03/17-03/30 |
- Wet Lab: Primers have arrived .PCR Amplification and then check PCR product on agarose gel.
- Dry Lab: For the project we were searching for paper to find out how to design model.
- Human practice: Asking expert for the advise of our project.
|
| 03/31-04/13 |
- Wet Lab: Ligase and overlap extension PCR.
- Dry Lab: We thought that the growth status of bacteria had the effect on our final product, so we planned to set up the growth model of E.coli first.
- Human practice: Meetup with different iGEM team and asking their experience about our current problem.
|
| 04/14-04/27 |
- Wet Lab: Heat Shock Transformation of Chemically Component E.coli( DH5α). Used PCR to colony, afterward Run the gel to do initial analysis, we could know from Agarose gel electrophoresis the results of expansion are mostly fit the size
- Dry Lab: We looked for the literature on the bacterial growth model and found that Gompertz model was recognized and recommended by many researchers.
- Human practice: Conduct a Questionnaire ranging from under18 and above 65 about public cognition.
|
| 04/28-05/11 |
- Wet Lab: DNA Sequencing (vector designed is Successful).
- Dry Lab: We asked the experimental group for help to do the growth curve of E.coli in general environment, and then we continued to study the Gompertz model.
- Human practice: Holding a camp for high school student, teaching them basic synthesis biology.
|
| 05/12-05/25 |
- Wet Lab: Designed primers for expression of Antimicrobial peptide.
- Dry Lab: We consolidated and analyzed the data from the experimental group, after that we set up our growth model based on the Gompertz model.
- Human practice: Visit different alumni like department of chemical engineering.
|
| 05/26-06/08 |
- Wet Lab: Designed primers for expression of Antimicrobial peptide.
- Dry Lab: We made a preliminary model of the growth model, but we still searched papers in the hope that the model could be perfected.
- Human practice: Target on tour project and ask help for more profession problem.
|
| 06/09-06/22 |
- Wet Lab: Primers have arrived .PCR Amplification and then check PCR product on agarose gel. Ligase. Ligase product was subjected to DNA extraction using a Gene-Spin TM 1-4-3 DNA Purification Kit, and plasmids were used PCR. Then Run the gel to do initial analysis.
- Dry Lab: We compared the improved model with the data from the experimental group.
- Human practice: Hold an activity to kindergarden student and make them know how fun the science is.
|
| 06/23-07/06 |
- Wet Lab: Heat Shock Transformation of Chemically Component C43 E. coli. Isolation of plasmid in E.coli(C43).
- Dry Lab: We wanted or maximize product effect at the lowest cost. So we designed some experiments in order to obtain the best convention and the suitable effective time of peptide.
- Human practice: Meet up with 2019 NCTU iGEM on video.
|
| 07/07-07/20 |
- Wet Lab: Used PCR to colony, afterward Run the gel to do initial analysis, we could know from Agarose gel electrophoresis the results of expansion are mostly fit the size.
- Dry Lab: We still search for information for suitable model references.
- Human practice: Speech to student of other major.
|
| 07/21-08/03 |
- Wet Lab: Protein Overexpression Protocol. And cell disruption.
- Dry Lab: We asked the teachers who came from the department of bioscience and chemistry for the design and some related questions on the experiments.
- Human practice: Meet up with 2019 mingdao iGEM onsite.
|
| 08/04-08/17 |
- Wet Lab: Protein Overexpression Protocol. And cell disruption..
- Dry Lab: We had an experimental discussion with the experimental group.
- Human practice: Take part in 2019 Taiwan conference.
|
| 08/18-08/31 |
- Wet Lab: protein purification
- Dry Lab: We assisted the experimental group in the experiment.
- Human practice: Collaborate with iGEM mingdao.
|
| 09/01-09/14 |
- Wet Lab: Growth curve measurement (Antimicrobial peptide)
- Dry Lab: We analyzed the data and tried to work out the equation.
- Human practice: Edit iGEM wiki page
|
| 09/15-09/28 |
- Wet Lab: MIC test.
- Dry Lab: We applied the computer software matlab try to make the figure of model.
- Human practice: Edit iGEM wiki page
|
| 09/29-10/12 |
- Wet Lab: protein purification
- Dry Lab: We asked the instructor whether the model right or not.
- Human practice: Edit iGEM wiki page.
|
| 10/12-10/26 |
- Wet Lab: Edit iGEM wiki page.
- Dry Lab: We discussed our final models and submitted the data.
- Human practice: Edit iGEM wiki page.
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